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Diabetologia

, Volume 42, Issue 4, pp 465–471 | Cite as

The effect of human proinsulin C-peptide on erythrocyte deformability in patients with Type I diabetes mellitus

  • T. Kunt
  • S. Schneider
  • A. Pfützner
  • K. Goitum
  • M. Engelbach
  • B. Schauf
  • J. Beyer
  • T. Forst
Rapid communication

Abstract

Aims/hypothesis. In recent years, evidence has arisen that proinsulin C-peptide exerts biological effects especially on microcirculation, e. g. C-peptide has been shown to increase skin microcirculation in patients with Type I (insulin-dependent) diabetes mellitus and to activate endothelial nitric oxide synthase. This study aimed to investigate the influence of proinsulin C-peptide on erythrocyte deformability which was assessed by means of laser diffractoscopy. Methods. Blood samples from healthy control subjects (n = 10) and Type I diabetic patients (n = 15) completely deficient of C-peptide were analysed at shear stresses ranging from 0.3 to 30 Pa. Results. Erythrocyte deformability was lower in the group of Type I diabetic patients than in the control subjects. Preincubation of the diabetic blood samples with various concentrations of human proinsulin C-peptide for 8 h restored the deformability of erythrocytes, almost reaching the values of control samples. In contrast, proinsulin C-peptide did not modify the erythrocyte deformability of control subjects. Conclusion/interpretation. We conclude that proinsulin C-peptide is able to ameliorate the impaired deformability of erythrocytes in Type I diabetic patients and we hypothesise that this effect is mediated by restoration of Na+-K+-ATPase activity, which is known to be attenuated in diabetic patients. [Diabetologia (1999) 42: 465–471]

Keywords Proinsulin C-peptide erythrocyte deformability microcirculation laser diffractoscopy shear stress. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • T. Kunt
    • 1
  • S. Schneider
    • 1
  • A. Pfützner
    • 1
  • K. Goitum
    • 1
  • M. Engelbach
    • 1
  • B. Schauf
    • 2
  • J. Beyer
    • 1
  • T. Forst
    • 1
  1. 1.Department of Endocrinology, University of Mainz, GermanyDE
  2. 2.Department of Obstetrics and Gynaecology, University of Giessen, GermanyDE

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