Advertisement

Diabetologia

, Volume 41, Issue 11, pp 1392–1396 | Cite as

Reduction of oxidative stress by oral N-acetyl-L-cysteine treatment decreases plasma soluble vascular cell adhesion molecule-1 concentrations in non-obese, non-dyslipidaemic, normotensive, patients with non-insulin-dependent diabetes

  • G. De Mattia
  • M. C. Bravi
  • O. Laurenti
  • M. Cassone-Faldetta
  • A. Proietti
  • O. De Luca
  • A. Armiento
  • C. Ferri
Short communication

Summary

To assess in vivo effects of antioxidants on vascular cell adhesion molecule (VCAM)-1 expression, circulating soluble VCAM-1 and intraerythrocytic reduced glutathione (GSH) and GSH disulphide (GSSG) concentrations were evaluated in non-insulin-dependent diabetic patients without complications (9 men, 6 women, 48 ± 6 years old) before and after 1 month of either oral N-acetyl-L-cysteine (1.200 mg/day) or placebo treatments, given in randomized, cross-over, double-blind fashion. Ten healthy subjects (7 men, 3 women, 52 ± 4 years old) served as control subjects. Baseline plasma VCAM-1 concentrations were higher (p = 0.007) in non-insulin-dependent diabetic patients (707.9 ± 52.5 ng/ml) than in control subjects (627.3 ± 84.6 ng/ml). Intraerythrocytic GSSG content was higher (non-insulin dependent diabetic patients: 0.618 ± 0.185 μmol/g Hb; control subjects: 0.352 ± 0.04 μmol/g Hb, p = 0.0002), whereas intraerythrocytic GSH concentrations were lower (p = 0.001) in non-insulin dependent diabetic patients (6.0 ± 0.7 μmol/g Hb) than in control subjects (7.1 ± 0.5 μmol/g Hb). The mean GSH:GSSG ratio was also lower (p = 0.0001) in the first (10.9 ± 4.5) than in the second group (20.2 ± 1.4). Circulating VCAM-1 and intraerythrocytic GSH concentrations were negatively correlated in non-insulin diabetic patients (r = 0.605, p = 0.01). Treatment with N-acetyl-L-cysteine decreased plasma VCAM-1 (p = 0.01) and intraerythrocytic GSSG (p = 0.006) but increased GSH concentrations (p = 0.04) and the GSH:GSSG ratio (p = 0.004) in non-insulin dependent diabetic patients. Our data indicate that the vascular endothelium is activated in non-insulin dependent diabetes. Antioxidant treatment counterbalanced such endothelial activation. Thus, antioxidant agents might protect against oxidant-related upregulation of endothelial adhesion molecules and slow down the progression of vascular damage in non-insulin dependent diabetes. [Diabetologia (1998) 41: 1392–1396]

Keywords Adhesion molecules endothelium vascular cell adhesion molecule-1 oxidants. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • G. De Mattia
    • 1
  • M. C. Bravi
    • 2
  • O. Laurenti
    • 1
  • M. Cassone-Faldetta
    • 2
  • A. Proietti
    • 1
  • O. De Luca
    • 1
  • A. Armiento
    • 1
  • C. Ferri
    • 1
  1. 1.Università“La Sapienza”, Fondazione Andrea Cesalpino, Roma, ItalyIT
  2. 2.Università di L'Aquila, Dipartimento di Medicina Interna, L'Aquila, ItalyIT

Personalised recommendations