Diabetologia

, Volume 41, Issue 6, pp 681–683

Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

  • H. E. Naserke
  • N. Dozio
  • A.-G. Ziegler
  • E. Bonifacio
Originals

DOI: 10.1007/s001250050968

Cite this article as:
Naserke, H., Dozio, N., Ziegler, AG. et al. Diabetologia (1998) 41: 681. doi:10.1007/s001250050968

Summary

Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 μl of serum was compared with that in a conventional radiobinding assay which uses 600 μl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95 %) of 239 samples tested were concordant, and IAA levels correlated well (r2 = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69 % and 98 % for the micro radiobinding assay and 72 % and 98 % for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children. [Diabetologia (1998) 41: 681–683]

Keywords Insulin autoantibodies radiobinding assay prediction insulin-dependent diabetes mellitus. 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. E. Naserke
    • 1
  • N. Dozio
    • 2
  • A.-G. Ziegler
    • 3
  • E. Bonifacio
    • 2
  1. 1.Institute of Diabetes Research, Munich, GermanyDE
  2. 2.Istituto Scientifico San Raffaele, Milan, ItalyIT
  3. 3.Institute of Diabetes Research and Academic Hospital Schwabing, Munich, GermanyDE

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