Diabetologia

, Volume 41, Issue 3, pp 292–299 | Cite as

The early phase of glucose-stimulated insulin secretion requires nitric oxide

  • G. A. Spinas
  • R. Laffranchi
  • I. Francoys
  • I. David
  • C. Richter
  • M. Reinecke
Originals

Summary

Nitric oxide (nitrogen monoxide, NO) acts as a signal transducer in a variety of cells. In the present study rat pancreatic islets were perifused with physiologically relevant glucose concentrations in the presence or absence of various NO-modulating agents. Perifusion in the presence of 0.1–1 mmol/l of the NO synthase inhibitor, NG-monomethyl-L-arginine or of 10 μmol/l of the NO-scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO), resulted in an inhibition of the early phase of glucose-stimulated insulin secretion by 60–65 % and 46 %, respectively. Light- and electron-microscopic studies revealed that pancreatic islets constitutively express NO-synthase in alpha and delta cells, where it is confined to the secretory granules. Therefore, these data indicate that NO may be important in the signal transduction pathway of the early phase of glucose-stimulated insulin secretion. [Diabetologia (1998) 41: 292–299]

Keywords Nitric oxide nitrogen monoxide insulin secretion glucose nitric oxide synthase alpha cell beta cell delta cell. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • G. A. Spinas
    • 1
  • R. Laffranchi
    • 1
  • I. Francoys
    • 1
  • I. David
    • 2
  • C. Richter
    • 3
  • M. Reinecke
    • 2
  1. 1.Division of Endocrinology and Diabetes, Department of Internal Medicine, University Hospital, Zürich, SwitzerlandCH
  2. 2.Division of Neuroendocrinology, Institute of Anatomy, University of Zürich-Irchel, Zürich, SwitzerlandCH
  3. 3.Laboratory of Biochemistry I, Swiss Federal Institute of Technology (ETH), Zürich, SwitzerlandCH

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