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Diabetologia

, Volume 40, Issue 6, pp 671–675 | Cite as

Genetic studies of neuropeptide Y and neuropeptide Y receptors Y1 and Y5 regions in morbid obesity

  • C. Roche
  • P. Boutin
  • C. Dina
  • G. Gyapay
  • A. Basdevant
  • J. Hager
  • B. Guy-Grand
  • K. Clément
  • P. Froguel

Summary

Synthesis and release of neuropeptide Y (NPY) are both regulated by leptin binding to its hypothalamic receptor mediating some of the effects of leptin on food intake. Moreover, NPY administration is a powerful stimulant of feeding behaviour. Thus, we investigated the potential implication of NPY, NPY-Y1 and -Y5 subtype receptors [rNPY-Y1/-Y5] in the development of human obesity. Two complementary genetic approaches were used: 1) linkage analyses between obesity and polymorphic markers located nearby NPY and rNPY-Y1/-Y5 genes (respectively on chromosomes 7p15.1 and 4q[31.3–32]) in 93 French Caucasian morbidly obese families; 2) single strand conformation polymorphism (SSCP) scanning of the coding region of the NPY and rNPY-Y1 genes performed in 50 unrelated obese patients ascertained on the basis of a body mass index of 27 kg/m2 or more and a family history of obesity. No evidence of linkage between morbid obesity or obesity-related quantitative traits and NPY and rNPY-Y1/Y5 regions was found in this population. Moreover, SSCP scanning revealed no mutation in the coding region of NPY and rNPY-Y1 genes among obese subjects. These results suggest that NPY and NPY-Y1/Y5 receptors are unlikely to be implicated in the development of human morbid obesity, at least in the French Caucasian population. [Diabetologia (1997) 40: 671–675]

Keywords Obesity genetic body mass index neuropeptide Y neuropeptide Y receptors leptin. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • C. Roche
    • 1
  • P. Boutin
    • 1
  • C. Dina
    • 1
  • G. Gyapay
    • 2
  • A. Basdevant
    • 3
  • J. Hager
    • 1
  • B. Guy-Grand
    • 3
  • K. Clément
    • 1
  • P. Froguel
    • 1
  1. 1.CNRS EP10, Institut Pasteur and CHU de Lille, Lille, FranceFR
  2. 2.Généthon and URA 1922, Every, FranceFR
  3. 3.Department of Nutrition – Hospital Hotel-Dieu, Paris, FranceFR

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