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Diabetologia

pp 1–10 | Cite as

Substrate metabolism, hormone and cytokine levels and adipose tissue signalling in individuals with type 1 diabetes after insulin withdrawal and subsequent insulin therapy to model the initiating steps of ketoacidosis

  • Thomas S. Voss
  • Mikkel H. Vendelbo
  • Ulla Kampmann
  • Steen B. Pedersen
  • Thomas S. Nielsen
  • Mogens Johannsen
  • Mads V. Svart
  • Niels Jessen
  • Niels Møller
Article

Abstract

Aims/hypothesis

Lack of insulin and infection/inflammation are the two most common causes of diabetic ketoacidosis (DKA). We used insulin withdrawal followed by insulin administration as a clinical model to define effects on substrate metabolism and to test whether increased levels of counter-regulatory hormones and cytokines and altered adipose tissue signalling participate in the early phases of DKA.

Methods

Nine individuals with type 1 diabetes, without complications, were randomly studied twice, in a crossover design, for 5 h followed by 2.5 h high-dose insulin clamp: (1) insulin-controlled euglycaemia (control) and (2) after 14 h of insulin withdrawal in a university hospital setting.

Results

Insulin withdrawal increased levels of glucose (6.1 ± 0.5 vs 18.6 ± 0.5 mmol/l), NEFA, 3-OHB (127 ± 18 vs 1837 ± 298 μmol/l), glucagon, cortisol and growth hormone and decreased HCO3 and pH, without affecting catecholamine or cytokine levels. Whole-body energy expenditure, endogenous glucose production (1.55 ± 0.13 vs 2.70 ± 0.31 mg kg−1 min−1), glucose turnover, non-oxidative glucose disposal, lipid oxidation, palmitate flux (73 [range 39–104] vs 239 [151–474] μmol/min), protein oxidation and phenylalanine flux all increased, whereas glucose oxidation decreased. In adipose tissue, Ser473 phosphorylation of Akt and mRNA levels of G0S2 decreased, whereas CGI-58 (also known as ABHD5) mRNA increased. Protein levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase phosphorylations were unaltered. Insulin therapy decreased plasma glucose concentrations dramatically after insulin withdrawal, without any detectable effect on net forearm glucose uptake.

Conclusions/interpretation

Release of counter-regulatory hormones and overall increased catabolism, including lipolysis, are prominent features of preacidotic ketosis induced by insulin withdrawal, and dampening of Akt insulin signalling and transcriptional modulation of ATGL activity are involved. The lack of any increase in net forearm glucose uptake during insulin therapy after insulin withdrawal indicates muscle insulin resistance.

Trial registration

ClinicalTrials.gov NCT02077348

Funding

This study was supported by Aarhus University and the KETO Study Group/Danish Agency for Science Technology and Innovation.

Keywords

Adipose tissue Cytokines Hormones Insulin Ketoacidosis Lipolysis 

Abbreviations

3-OHB

3-hydroxybutyrate (β-hydroxybutyrate)

ATGL

Adipose triglyceride lipase

CGI-58

Comparative gene identification-58

DKA

Diabetic ketoacidosis

EGP

Endogenous glucose production

FBF

Forearm blood flow

HSL

Hormone-sensitive lipase

LPS

Lipopolysaccharide

NOGD

Non-oxidative glucose disposal

OGD

Oxidative glucose disposal

PKA

Protein kinase A

Notes

Acknowledgements

The authors thank the following people at Aarhus University Hospital, Denmark for their excellent technical assistance: A. Mengel, K. Nyborg Rasmussen, E. Søgaard Hornemann and K. Mathiassen, Medical Research Laboratory; H. Zibrandtsen, Research Laboratory for Biochemical Pathology; L. Pedersen, Department of Endocrinology and Internal Medicine.

Contribution statement

TSV recruited the participants, conducted the trial and performed the statistical analyses. TSV, MHV, UK, NJ and NM contributed to conception and design of the study. NM, NJ, MVS, MJ, SBP, TSN and TSV collected and interpreted the data. TSV and NM drafted the manuscript and all authors revised it critically and approved the final version to be published. NM is the guarantor of this work and, as such, takes responsibility for the integrity of the data and the accuracy of the data analyses.

Funding

This study was supported by Aarhus University and the KETO Study Group/Danish Agency for Science Technology and Innovation (grant no. 0603-00479 [to NM]).

Duality of interest

The authors declare that there is no duality of interest associated with this manuscript.

Supplementary material

125_2018_4785_MOESM1_ESM.pdf (119 kb)
ESM Methods (PDF 119 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Thomas S. Voss
    • 1
    • 2
  • Mikkel H. Vendelbo
    • 3
  • Ulla Kampmann
    • 2
  • Steen B. Pedersen
    • 2
  • Thomas S. Nielsen
    • 4
  • Mogens Johannsen
    • 5
  • Mads V. Svart
    • 1
    • 2
  • Niels Jessen
    • 6
    • 7
  • Niels Møller
    • 1
    • 2
  1. 1.Medical Research LaboratoryAarhus UniversityAarhus CDenmark
  2. 2.Department of Endocrinology and Internal MedicineAarhus University HospitalAarhusDenmark
  3. 3.Department of Nuclear MedicineAarhus University HospitalAarhusDenmark
  4. 4.The Novo Nordisk Foundation Center for Basic Metabolic Research, Section on Integrative Physiology, Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark
  5. 5.Section for Forensic Chemistry, Department of Forensic MedicineAarhus UniversityAarhusDenmark
  6. 6.Research Laboratory for Biochemical Pathology and Department of BiomedicineAarhus UniversityAarhusDenmark
  7. 7.Department of Clinical PharmacologyAarhus University HospitalAarhusDenmark

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