Basal fatty acid oxidation increases after recurrent low glucose in human primary astrocytes
Hypoglycaemia is a major barrier to good glucose control in type 1 diabetes. Frequent hypoglycaemic episodes impair awareness of subsequent hypoglycaemic bouts. Neural changes underpinning awareness of hypoglycaemia are poorly defined and molecular mechanisms by which glial cells contribute to hypoglycaemia sensing and glucose counterregulation require further investigation. The aim of the current study was to examine whether, and by what mechanism, human primary astrocyte (HPA) function was altered by acute and recurrent low glucose (RLG).
To test whether glia, specifically astrocytes, could detect changes in glucose, we utilised HPA and U373 astrocytoma cells and exposed them to RLG in vitro. This allowed measurement, with high specificity and sensitivity, of RLG-associated changes in cellular metabolism. We examined changes in protein phosphorylation/expression using western blotting. Metabolic function was assessed using a Seahorse extracellular flux analyser. Immunofluorescent imaging was used to examine cell morphology and enzymatic assays were used to measure lactate release, glycogen content, intracellular ATP and nucleotide ratios.
AMP-activated protein kinase (AMPK) was activated over a pathophysiologically relevant glucose concentration range. RLG produced an increased dependency on fatty acid oxidation for basal mitochondrial metabolism and exhibited hallmarks of mitochondrial stress, including increased proton leak and reduced coupling efficiency. Relative to glucose availability, lactate release increased during low glucose but this was not modified by RLG. Basal glucose uptake was not modified by RLG and glycogen levels were similar in control and RLG-treated cells. Mitochondrial adaptations to RLG were partially recovered by maintaining euglycaemic levels of glucose following RLG exposure.
Taken together, these data indicate that HPA mitochondria are altered following RLG, with a metabolic switch towards increased fatty acid oxidation, suggesting glial adaptations to RLG involve altered mitochondrial metabolism that could contribute to defective glucose counterregulation to hypoglycaemia in diabetes.
KeywordsAdenosine triphosphate AMP-activated protein kinase Astrocyte Diabetes Fatty acid oxidation Glia Hypoglycaemia Lactate Low glucose Mitochondrial metabolism
AMP-activated protein kinase
Carnitine palmitoyltransferase 1
Extracellular acidification rate
Fatty acid oxidation
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
Glial fibrillary acidic protein
Human primary astrocyte
Mitochondrial pyruvate carrier
Oxygen consumption rate
Recurrent low glucose
Voltage-dependent anion channel
Ventromedial nucleus of the hypothalamus
Hypoglycaemia remains a major concern for people with type 1 diabetes and advanced insulin-treated type 2 diabetes. For individuals with diabetes, hypoglycaemia is a major barrier to enjoying the benefits of exercise [1, 2] and is increasingly thought to increase the risk of cardiovascular events in both type 1 and type 2 diabetes [3, 4]. Individuals who experience frequent hypoglycaemia or have long-duration diabetes produce diminished counterregulatory responses (CRRs) to hypoglycaemia [5, 6], often accompanied by reduced symptom awareness, termed ‘impaired hypoglycaemia awareness’.
Defective glucose CRRs to hypoglycaemia are, at least in part, mediated by changes in the function of critically important glucose-sensing neurons in the ventromedial nucleus of the hypothalamus (VMH), although glucose-sensing neurons in the hindbrain have also been implicated . In addition to neurons, the brain contains at least as many cells that lack electrically excitable membranes. Of these, the largest group is the glial cells, the largest subpopulation of which are astrocytes. As well as exhibiting a range of cell-to-cell signalling pathways, astrocytes are increasingly recognised as important players in central nervous system-based diseases, such as obesity , Alzheimer’s disease , Parkinson’s disease  and motor neuron disease .
Astrocytes play an important role in regulating glucose uptake into cerebral microvessels . In addition to this role in glucose delivery, more recent studies have demonstrated that stimuli such as 2-deoxyglucose, that block intracellular glucose metabolism, increase intracellular Ca2+ in hindbrain astrocytes , a well-accepted marker of increased astrocyte activity, suggesting a more direct role in sensing changes in glucose levels. In the hypothalamus, astrocyte activation by fasting is indicated by increased glial fibrillary acidic protein (GFAP) expression and process complexity [14, 15], both markers of gliosis.
Astrocytes contain small amounts of glycogen that can be broken down to glucose and metabolised to lactate, which is released to feed active neurons. For example, astrocytic glycogen stores can maintain axonal activity during energetic stress in white matter [16, 17] and also contribute to memory formation [18, 19]. Brain glycogen concentrations are increased on recovery from exercise , suggesting that astrocytic glycogen stores are both physiologically and pathophysiologically regulated. Astrocytes are in intimate apposition to the cerebral vasculature, meaning that a change in glycaemia may be detected by astrocytes before neurons. Moreover, astrocytes are generally accepted as metabolically flexible neural cells that can rapidly upregulate glycolysis to support neuronal function, partly through the higher expression of 6-phosphofructo-2-kinase type 3 (PFKFB3), which can stimulate glycolysis . Despite this, little is known about the intrinsic response of astrocytes to acute and recurrent low glucose (RLG) that occurs in type 1 diabetes.
Given that astrocytes are metabolically flexible cells, we hypothesised that human astrocytes would: (1) sense low glucose by increasing phosphorylation of cellular energy sensor AMP-activated protein kinase (AMPK); and (2) exhibit altered glycolytic and mitochondrial metabolism following RLG. To test this, we utilised both human primary astrocytes (HPA) and U373 astrocytoma cells and measured directly components of glycolytic and mitochondrial metabolism, lactate release, glycogen levels, intracellular nucleotide levels and expression of key metabolic enzymes.
Astrocyte isolation and cell culture
HPA were isolated from a 17 year old male donor from subventricular deep white matter tissue immediately post-mortem following consent from next of-kin and with ethical approval from the North and East Devon Research Ethics Committee. Astrocytes were isolated from tissue blocks as previously described , subcultured and frozen stocks prepared. HPA cells were maintained in humidified incubators with 95% O2/5% CO2 in HPA stock media. U373 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). See electronic supplementary material (ESM) Methods for further details of stock media components.
For experiments, the glucose concentration was reduced to 2.5 mmol/l glucose for 2 h, supplemented with 1% serum and 22.5 mmol/l mannitol for HPA cells. Glucose concentrations were then dropped further to 0.1 mmol/l glucose as a hypoglycaemic-like stimulus or plated at 2.5 mmol/l glucose, supplemented with 1% (vol./vol.) serum and 24.9 mmol/l or 22.5 mmol/l mannitol, for HPA and U373 cells, respectively. For RLG studies over 4 days, control cells were exposed to zero bouts (2.5 mmol/l glucose instead) and RLG cells exposed to 4 bouts of 3 h of 0.1 mmol/l glucose and recovered in media containing high (HPA, 5.5 mmol/l; U373, 7.5 mmol/l) glucose and FBS overnight (see ESM Fig. 1 for culture model and ESM Methods for further details). The reasons for this were twofold: (1) higher glucose levels overnight (approximately 19 h) prevented glucose depletion from the media, which would occur if the cells were maintained at 2.5 mmol/l; and (2) this pattern more accurately represented a model of glucose variation seen in type 1 diabetes.
All cell lines used were verified as mycoplasma-free using a commercial test kit (MycoAlert, Lonza, Slough, UK). Cells were randomly assigned to experimental groups by an unblinded investigator (unless otherwise stated).
Cellular protein was isolated as previously described , with minor modifications. Briefly, for acute studies 4.5 × 105 cells or, for RLG studies, 2.5 × 105 cells were seeded on to 60 mm petri dishes and harvested in 65 μl lysis buffer. Protein concentrations were assessed by Bradford assay. A 10 μg sample of protein was loaded per lane on 7–10% (wt/vol.) polyacrylamide gels. Proteins were transferred to nitrocellulose membranes, blocked with BSA (5% wt/vol.) or powdered milk (5% wt/vol.) and probed with antibodies against target proteins. See ESM Methods for antibody details.
Analysis of cellular metabolism
HPA and U373 cells were seeded at 2 × 104 cells per well in Seahorse XFe96 assay plates (no. 102416-100, Agilent, Craven Arms, UK) on the day before the study. Medium was exchanged for low-buffered media, the pH adjusted to 7.40 at 37.0°C and cells placed in an atmospheric CO2 incubator, to remove CO2 buffering capacity, 1 h prior to starting the assay. Mitochondrial stress tests (no. 103015-100, Agilent) and mitochondrial fuel flexibility tests (no. 103270-100, Agilent) were performed as per manufacturer’s instructions (see ESM Methods for further details). Samples with negative values for OCR (probably due to the presence of an air bubble) were excluded from analysis.
Measurement of intracellular nucleotides
Total ATP levels were measured using ATPlite (no. 6016941, Perkin Elmer, Seer Green, UK) with minor modifications as previously described . ATP/ADP ratios were assayed by luminescence assay (no. MAK135, Sigma-Aldrich, Gillingham, UK). Briefly, 1 × 103 cells were seeded on to black-walled 96 well plates and exposed to 2.5 mmol/l or 0.1 mmol/l glucose levels for 15–180 min and nucleotide ratios assayed as per manufacturer’s instructions.
Measurement of extracellular lactate, glucose uptake and glycogen levels
Extracellular lactate was measured by assessing NADH production from NAD+ in the presence of lactate dehydrogenase as previously described . Briefly, 100 μl of extracellular supernatant fraction was examined against a standard curve of lactate from 0 nmol/l to 50 nmol/l. Glucose uptake was measured using fluorescently labelled glucose analogue 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-deoxyglucose (6-NBDG; CAY13961-5/6-NBDG, Cayman Chemical, Tallinn, Estonia). 6-NBDG (600 μmol/l) was added for 15 min in 2.5 mmol/l glucose in control conditions and in cells exposed to RLG. Glycogen levels were measured using a fluorometric kit as per manufacturer’s instructions (no. MET-5023, Cambridge Bioscience, Cambridge, UK).
Mitochondrial morphology was examined by staining HPA cells with MitoTracker Red CMXRos (50 nmol/l; M7512, no. 1785958, Thermo Fisher Scientific, Loughborough, UK) before fixing and imaging using confocal microscopy (Leica, London, UK DMi8; ×63/oil immersion lens) by an investigator blind to sample identity. See ESM Methods for further details. To examine intermediate filament protein expression/localisation, HPA cells were fixed and stained with an anti-vimentin antibody (V6630, no. 102M4831, mouse, Sigma-Aldrich; 1:500 in lysine buffer) or an anti-GFAP antibody (z0334 no. 20035994, rabbit, Agilent; 1:100 in lysine buffer). All antibodies were validated by the manufacturer. See ESM Methods for further details.
For immunoblotting, densitometric values were normalised to unity to examine relative fold change in expression. A one-sample t test was used to determine significant changes in phosphorylation or expression, relative to control. For comparisons of two groups a two-tailed unpaired t test was used and for multiple group comparisons, a one-way ANOVA with post hoc Bonferroni were used. To compare the mean differences between groups split by two independent variables, a two-way ANOVA with Bonferroni multiple comparisons tests was used. Statistical tests were performed using GraphPad Prism software (Prism 5; GraphPad Software, La Jolla, CA, USA). Results are expressed as mean ± standard error, unless otherwise stated. Values of p < 0.05 were considered statistically significant.
AMPK is activated by acute low glucose in human astrocytes
RLG exposure increases mitochondrial metabolism in human astrocytes
To determine whether baseline metabolism was altered following RLG, we performed mitochondrial stress tests on HPA and U373 cells (see ESM Fig. 1 for RLG model). In order to observe any cellular adaptations, this was performed in 2.5 mmol/l glucose, representing euglycaemic, glucose levels following RLG.
In addition to changes in OCR, we also noted significantly elevated basal extracellular acidification rate (ECAR), in HPA cells exposed to RLG (Fig. 2i, k), indicating enhanced glycolysis following RLG. This did not occur within U373 cells following RLG (Fig. 2j, l).
Astrocytes exposed to RLG display enhanced glycolysis on recovery of glucose level
Fatty acid oxidation is increased in human astrocytes following RLG
Human astrocytes maintain energy production (total ATP and ATP/ADP ratio) during low glucose conditions
RLG does not alter glucose uptake or glycogen levels in human astrocytes
We next assessed the degree of glucose uptake by human astrocytes using the fluorescently labelled glucose analogue, 6-NBDG. HPA cells exposed to RLG showed no change in 6-NBDG uptake (Fig. 6e), indicating that the persistent changes in mitochondrial oxygen consumption were not simply mediated by increased glucose uptake. Correspondingly, we did not observe any changes in astrocytic glycogen content following RLG (Fig. 6f).
Correlating with our mitochondrial imaging studies, the levels of mitochondrial markers succinate dehydrogenase (SDH) and the voltage-dependent anion channel (VDAC) were not altered by RLG, suggesting no change in total mitochondrial content (ESM Fig. 5). Fatty acid synthase (FAS) and CPT-1a expression were also not altered, indicating that changes in fatty acid-dependency following RLG are likely to be mediated by metabolic flux/activity rather than alternations in expression of enzymes within the FAO pathway.
Partial recovery of metabolic changes by maintenance of glucose levels
Our studies shed new light on the previously uncharacterised intrinsic metabolic changes in human glial cells in response to acute and recurrent low glucose. We demonstrate that astrocytes, in the absence of physical and chemical signals from neurons, react to low glucose by increasing the activation of the key metabolic sensor AMPK. Changes in AMPK pathway phosphorylation occur within a pathophysiologically relevant glucose concentration range, similar to that which affects CNS glucose-sensing neurons .
It is well accepted that astrocytes are more metabolically flexible than neurons, partly because of the higher expression of PFKFB3, which can accelerate glycolysis during metabolic stress . Therefore, to examine specifically the changes in astrocyte metabolism following RLG, we measured markers of mitochondrial and glycolytic metabolism in HPA monocultures. Importantly, we found that daily prior bouts of low glucose (4 × 0.1 mmol/l glucose), lasting 3 h, were sufficient to increase basal mitochondrial and glycolytic metabolism to sustain intracellular ATP supply. Increased basal mitochondrial metabolism is likely mediated, at least in part, by an increased contribution of FAO to basal oxygen consumption. Correlating with this hypothesis, we noted increased mitochondrial proton leak and reduced mitochondrial coupling efficiency in astrocytes exposed to RLG. Importantly, FAO is known to increase uncoupling, as FAO generates increased levels of reactive oxygen species (ROS)/superoxide  that can increase proton leak via activation of uncoupling proteins or the ATP/ADP antiporter (ANT; ). Previous work has demonstrated that, in vivo, genes for β-oxidation are induced by acute hypoglycaemia, but this fails following recurrent hypoglycaemia . Our data in astrocyte monocultures contrast with these measurements taken from rodent mediobasal hypothalamus, which include both neurons and glia and axon projections tracking through the dissected area. Therefore, it is possible that cell-type-specific changes are masked when measuring mRNA expression from whole-tissue sections.
A previous study demonstrated that low intracellular glucose levels caused by loss of insulin receptors on astrocytes (leading to reduced astrocytic glucose transport) resulted in astrocytic mitochondria that were smaller and less numerous , suggesting increased mitophagy. In our study, despite evidence of mitochondrial stress and in contrast to our expectation, examination of the filamentous mitochondrial networks revealed no change in either mitochondrial size or number following acute low glucose or RLG. Indeed, mitochondria exposed to RLG generally displayed a normal filamentous network, with little or no evidence of mitochondrial fragmentation. These data suggest that astrocyte mitochondria successfully adapt to maintain energy production in response to repeated bouts of low glucose availability. It is possible that the relatively acute reductions in glucose availability in our experiments were a mild stress, insufficient to cause gross changes in mitochondrial morphology. Moreover, cells were recovered into solutions containing serum, likely providing sufficient insulin/growth factor receptor activation to aid recovery from the acute stress. Therefore, it is possible that reductions in insulin receptor activation, rather than just intracellular glucose deprivation per se, may mediate the altered mitochondrial morphology reported previously. A similar phenotype of increased mitochondrial fragmentation was also reported using inducible knockdown of insulin-like growth factor 1 (IGF-1) receptors on astrocytes , suggesting an important role for hormones in regulating glial cell mitochondrial function that requires further investigation.
Previous studies in rats found no change in brain glycogen levels following recurrent hypoglycaemia  or any correlation between brain glycogen content and impaired awareness of hypoglycaemia in humans . However, other studies suggest brain glycogen content is increased following recovery from insulin-induced hypoglycaemia  and following recurrent 2-deoxyglucose-induced glucoprivation (reduction in utilisable glucose) in mice . Our data clearly demonstrate that astrocytes intrinsically adapt by increasing cellular metabolism in response to recurrent energy stress. Specifically, rates of extracellular acidification (a marker for glycolysis) following glucose ‘reperfusion’ were elevated in cells exposed to RLG, suggesting an augmented re-activation of glycolysis. However, despite glycolysis being a major determinant of glycogen synthesis, we found no significant change in astrocytic glycogen levels following RLG. Extracellular lactate, which can be generated from glycogen breakdown during aglycaemia , increased substantially, relative to glucose availability, during low glucose exposure, though the magnitude of this response was not significantly altered following RLG. We cannot rule out, however, the possibility that more subtle changes in lactate and glycogen occur during the transition to low glucose or on glucose recovery. Moreover, before definitively ruling out a role for astrocytic glycogen and subsequent lactate release, a more replete in vitro model, containing both astrocytes and neurons, should be tested, thus facilitating the inclusion of neurotransmitter dynamics in the model. The ability to produce increasingly credible human neurons and astrocytes from inducible pluripotent stem cell (iPSC) sources could potentially enable such investigations to be performed in an in vitro human system, overcoming some of the physiological limitations of mono-culture systems.
Astrocytes play a key role in maintaining glutamatergic neurotransmission through the glutamate−glutamine cycle (for recent review see Schousboe ). Importantly, astrocytic glutamate clearance requires co-transport of three Na+ ions , meaning that, via the ATP-dependent Na+/K+ ATPase, there is a significant metabolic cost for astrocytes to maintain their Na+ homeostasis, estimated to account for approximately 20% of total astrocytic ATP production . This process may become particularly costly during hypoglycaemia. Moreover, glutamate stimulation of astrocytes produces mitochondrial dysfunction, decreasing mitochondrial spare respiratory capacity and increasing lactate production . Furthermore, recent observations suggest that astrocytic glutamate recycling is diminished following recurrent hypoglycaemia, a change most likely mediated by reduced astrocyte glutamate uptake . Interestingly, recurrent hypoglycaemia is associated with relative GABA excess within the VMH . In addition to glutamate, astrocytes also clear GABA from the extracellular space , which is energetically less expensive for the astrocytes than glutamate recycling . This raises the interesting possibility that the glutamatergic failure and relative GABA excess following recurrent hypoglycaemia may be mediated by astrocytes, plausibly as a protective mechanism against excitotoxicity, although further study is required.
Our data add to published findings and suggest that metabolic changes within astrocytes could be protective adaptations to limit brain cell damage or contribute to defective counterregulatory responses following RH. In support of the latter, astrocytes are increasingly recognised as playing an important role in the regulation of whole-body metabolism. For example, chemogenetic activation (by increasing intracellular Ca2+) of astrocytes in the arcuate nucleus increases feeding in mice and, conversely, sequestering astrocytic Ca2+ decreases food intake . Moreover, astrocytes can be a source of pro-inflammatory cytokines in the brain , including TNFα, which has previously been reported to blunt glucose-sensing glucose-inhibited neurons , suggesting that pro-inflammatory signals from astrocytes may regulate energy-sensing neurons of the hypothalamus . Accordingly, modulation of inflammatory signalling in astrocytes modulates feeding in response to a high-fat diet . Within the hindbrain, astrocytes can detect hypoglycaemia to regulate adjacent neurons to increase gastric emptying in response to a glucoprivic stimulus . Astrocytes have also been shown to decrease hyperglycaemia in a rodent model of type 1 diabetes , suggesting that astrocytes may play fundamental roles in regulating whole-body glucose levels. It should also be noted that there is significant heterogeneity in astrocytes across different brain regions. For example, hypothalamic astrocytes express more genes for lipid oxidation compared with cortical astrocytes, which express more genes for lipid synthesis . Regional differences in astrocytic glycogen content also exist, with high levels in hippocampus and cortex and comparatively lower levels in subcortical structures . Within the hindbrain, only a subset of astrocytes respond to a glucoprivic stimulus , indicating that not all astrocytes will respond to a given stimulus in the same manner. Given the significant heterogeneity of neurons within the hypothalamus, it is plausible that subtypes of astrocytes within this region may contribute distinct roles in hypoglycaemia sensing. However, the territory of a single astrocyte is likely to overlap with multiple neuronal types, probably requiring subcellular site-specific responses.
In conclusion, our data further support the evidence that glial cells detect hypoglycaemic stress. Moreover, we demonstrate that mitochondrial function (increased FAO) in human astrocytes is altered by RLG. These data suggest a possible role for glial mitochondrial adaptations in defective glucose counterregulation or protection against hypoglycaemia, and highlight the need for more investigation into neuron-glial interactions during and following recurrent hypoglycaemia.
We thank the family of the donor for making this research possible and N. Gutowski and J. Holley (both University of Exeter, Exeter, UK) for kindly gifting the human primary astrocytes. We also wish to thank N. Howe (Seahorse Bioscience, Agilent, UK) for guidance with extracellular flux analysis.
Parts of this study were presented at the American Diabetes Association 77th Scientific Sessions and at the Diabetes UK Annual Professional Conference 2017 and 2018.
PGWP, JMVW and JLR contributed to data acquisition. PGWP, JMVW, JLR, JKC, RW, ADR, KLJE and CB contributed to data analysis and interpretation. All authors contributed to drafting and revising the article and gave approval for the final version to be published. CB conceived the study and is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
This study was funded by grants from: Diabetes UK (RD Lawrence Fellowship to CB; 13/0004647); the Medical Research Council (MR/N012763/1) to KLJE, ADR and CB; and a Mary Kinross Charitable Trust PhD studentship to CB, ADR and RW to support PGWP. Additional support for this work came from awards from the British Society for Neuroendocrinology (to CB and KLJE), the Society for Endocrinology (CB), Tenovus Scotland (CB) and the University of Exeter Medical School (CB and KLJE). AR was also supported by a Royal Society Industry Fellowship.
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
- 1.Michaud I, Henderson M, Legault L, Mathieu ME (2017) Physical activity and sedentary behavior levels in children and adolescents with type 1 diabetes using insulin pump or injection therapy – the importance of parental activity profile. J Diabetes Complicat 31:381–386. https://doi.org/10.1016/j.jdiacomp.2016.11.016 CrossRefPubMedGoogle Scholar
- 16.Wender R, Brown AM, Fern R, Swanson RA, Farrell K, Ransom BR (2000) Astrocytic glycogen influences axon function and survival during glucose deprivation in central white matter. J Neurosci 20:6804–6810. https://doi.org/10.1523/JNEUROSCI.20-18-06804.2000 CrossRefPubMedGoogle Scholar
- 32.Herzog RI, McNay EC, McCrimmon R et al (2006) Effect of acute and recurrent hypoglycemia on regional changes in brain glycogen concentration. Diabetes 55:A149–A149Google Scholar
- 36.Schousboe A (2018) Metabolic signaling in the brain and the role of astrocytes in control of glutamate and GABA neurotransmission. Neurosci Lett. https://doi.org/10.1016/j.neulet.2018.01.038
- 37.Levy LM, Warr O, Attwell D (1998) Stoichiometry of the glial glutamate transporter GLT-1 expressed inducibly in a Chinese hamster ovary cell line selected for low endogenous Na+-dependent glutamate uptake. J Neurosci 18:9620–9628. https://doi.org/10.1523/JNEUROSCI.18-23-09620.1998 CrossRefPubMedGoogle Scholar
- 38.Silver IA, Erecinska M (1997) Energetic demands of the Na+/K+ ATPase in mammalian astrocytes. Glia 21:35–45. https://doi.org/10.1002/(SICI)1098-1136(199709)21:1<35::AID-GLIA4>3.0.CO;2-0 CrossRefPubMedGoogle Scholar
- 42.Iversen LL, Neal MJ (1968) The uptake of [3H]GABA by slices of rat cerebral cortex. J Neurochem 15(10):1141–1149. https://doi.org/10.1111/j.1471-4159.1968.tb06831.x CrossRefPubMedGoogle Scholar
- 45.Hao L, Sheng Z, Potian J, Deak A, Rohowsky-Kochan C, Routh VH (2016) Lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNFα) blunt the response of neuropeptide Y/agouti-related peptide (NPY/AgRP) glucose inhibited (GI) neurons to decreased glucose. Brain Res 1648:181–192. https://doi.org/10.1016/j.brainres.2016.07.035 CrossRefPubMedPubMedCentralGoogle Scholar
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.