Diabetologia

, Volume 60, Issue 9, pp 1834–1835 | Cite as

Autoantibody binding in liquid phase to IL-2 in human sera is not type 1 diabetes specific

  • Ilaria Marzinotto
  • Daniela Liberati
  • Cristina Brigatti
  • Riccardo Bonfanti
  • Angela Stabilini
  • Paolo Monti
  • Emanuele Bosi
  • Lorenzo Piemonti
  • Vito Lampasona
Research Letter

Keywords

Autoantibodies Autoimmunity ELISA IL-2 Immunoprecipitation LIPS Type 1 diabetes 

Abbreviations

LIPS

Luciferase immunoprecipitation system

NLuc-IL-2

Recombinant IL-2 tagged with a NanoLuc luciferase reporter

RBA

Radiobinding assay

ROC-AUC

Area under the receiver operator characteristic curve

Notes

Acknowledgements

We wish to thank the BioBank of the Immunohematology and Transfusion Medicine Unit, led by L. Santoleri at the San Raffaele Scientific Institute, Milan, Italy, for their assistance in sample collection and retrieval.

Data availability

Data generated in this study are available from the authors upon request.

Funding

This study was conducted within the framework of the Italian Ministry of Research’s project ‘Ivascomar project, Cluster Tecnologico Nazionale Scienze della Vita ALISEI’.

Duality of interest

The authors declare that there is no duality of interest associated with this manuscript.

Contribution statement

IM, DL, CB, RB, AS and PM acquired and analysed the data. EB, LP and VL designed the study, analysed the data and wrote the manuscript. All authors revised and approved the final version of the manuscript. VL is responsible for the integrity of the work as a whole.

References

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    Pérol L, Lindner JM, Caudana P et al (2016) Loss of immune tolerance to IL-2 in type 1 diabetes. Nat Commun 7:13027CrossRefPubMedPubMedCentralGoogle Scholar
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    Burbelo PD, Hirai H, Leahy H et al (2008) A new luminescence assay for autoantibodies to mammalian cell–prepared insulinoma-associated protein 2. Diabetes Care 31:1824–1826CrossRefPubMedPubMedCentralGoogle Scholar
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    Lampasona V, Ferrari M, Bosi E et al (1994) Sera from patients with IDDM and healthy individuals have antibodies to ICA69 on western blots but do not immunoprecipitate liquid phase antigen. J Autoimmun 7:665–674CrossRefPubMedGoogle Scholar
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    Liu E, Eisenbarth GS (2007) Accepting clocks that tell time poorly: fluid-phase versus standard ELISA autoantibody assays. Clin Immunol 125:120–126CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Ilaria Marzinotto
    • 1
  • Daniela Liberati
    • 2
  • Cristina Brigatti
    • 1
  • Riccardo Bonfanti
    • 3
  • Angela Stabilini
    • 1
  • Paolo Monti
    • 1
  • Emanuele Bosi
    • 1
    • 4
  • Lorenzo Piemonti
    • 1
    • 4
  • Vito Lampasona
    • 2
  1. 1.Diabetes Research Institute, IRCCS San Raffaele Scientific InstituteMilanItaly
  2. 2.Division of Genetics and Cell BiologyIRCCS San Raffaele Scientific InstituteMilanItaly
  3. 3.Department of PediatricsIRCCS San Raffaele Scientific InstituteMilanItaly
  4. 4.Vita-Salute UniversityMilanItaly

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