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Diabetologia

, Volume 58, Issue 1, pp 19–30 | Cite as

Roles of TBC1D1 and TBC1D4 in insulin- and exercise-stimulated glucose transport of skeletal muscle

  • Gregory D. Cartee
Review

Abstract

This review focuses on two paralogue Rab GTPase activating proteins known as TBC1D1 Tre-2/BUB2/cdc 1 domain family (TBC1D) 1 and TBC1D4 (also called Akt Substrate of 160 kDa, AS160) and their roles in controlling skeletal muscle glucose transport in response to the independent and combined effects of insulin and exercise. Convincing evidence implicates Akt2-dependent TBC1D4 phosphorylation on T642 as a key part of the mechanism for insulin-stimulated glucose uptake by skeletal muscle. TBC1D1 phosphorylation on several insulin-responsive sites (including T596, a site corresponding to T642 in TBC1D4) does not appear to be essential for in vivo insulin-stimulated glucose uptake by skeletal muscle. In vivo exercise or ex vivo contraction of muscle result in greater TBC1D1 phosphorylation on S237 that is likely to be secondary to increased AMP-activated protein kinase activity and potentially important for contraction-stimulated glucose uptake. Several studies that evaluated both normal and insulin-resistant skeletal muscle stimulated with a physiological insulin concentration after a single exercise session found that greater post-exercise insulin-stimulated glucose uptake was accompanied by greater TBC1D4 phosphorylation on several sites. In contrast, enhanced post-exercise insulin sensitivity was not accompanied by greater insulin-stimulated TBC1D1 phosphorylation. The mechanism for greater TBC1D4 phosphorylation in insulin-stimulated muscles after acute exercise is uncertain, and a causal link between enhanced TBC1D4 phosphorylation and increased post-exercise insulin sensitivity has yet to be established. In summary, TBC1D1 and TBC1D4 have important, but distinct roles in regulating muscle glucose transport in response to insulin and exercise.

Keywords

Akt substrate of 160 kDa Diabetes Glucose transport High-fat diet Insulin resistance Obesity Physical activity Review 

Abbreviations

AMPK

5' AMP-activated kinase

APPL2

Adaptor protein containing PH domain, PTB domain and leucine zipper motif 2

CaMKKα

Ca2+/calmodulin-dependent protein kinase kinase α

CBD

Calmodulin-binding domain

EDL

Extensor digitorum longus muscle

GAP

GTPase-activating protein

GSK3

Glycogen synthase kinase 3

LFD

Low-fat diet

HFD

High-fat diet

PAS

Phospho Akt substrate

PI3K

Phosphatidylinositol 3-kinase

PP2A

Protein phosphatase 2A

PTB

Phosphotyrosine-binding

RSK1

90 kDa ribosomal S6 kinase 1

SGK1

Serum- and glucocorticoid-responsive kinase 1

S/T kinase

Serine/threonine kinase

TA

Tibialis anterior muscle

TBC1D

Tre-2/BUB2/cdc 1 domain family

\( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)

Peak oxygen consumption

WT

Wild type

Introduction

The most important physiological stimuli for increasing skeletal muscle glucose transport are insulin and exercise [1, 2, 3, 4]. Each independently triggers the redistribution of GLUT4 transporters from the cell interior to cell surface membranes [4, 5]. In addition, a single exercise session can induce a subsequent increase in insulin-stimulated glucose transport in muscle and improved whole body insulin sensitivity for up to ~2–48 h post exercise [6, 7, 8, 9, 10]. This review focuses on two paralogue Rab-GTPase-activating proteins (Rab GAPs) that are members of the tre-2/BUB2/cdc 1 domain family (TBC1D; TBC1D1 and TBC1D4 [also known as Akt substrate of 160 kDa, AS160]), and their roles in regulating muscle glucose transport stimulated by insulin and/or exercise.

Early research on TBC1D1 and TBC1D4 phosphorylation used a commercially available polyclonal antibody (the phospho Akt substrate [PAS] antibody) that was created to react with phosphorylated S/T residues in Akt phosphomotifs on many proteins to identify novel Akt substrates [11, 12, 13, 14, 15]. Because there are multiple potential PAS-reactive phosphomotifs on TBC1D1 and TBC1D4 (proteins separated by only ~10 kDa using SDS-PAGE), care is needed when interpreting anti-PAS bands in immunoblots. In this review, numbers identifying amino acids refer to the human protein unless otherwise specified. Anti-PAS was found to have high affinity for TBC1D4 phosphorylated on T642 (pT642-TBC1D4) and lower affinity for TBC1D4 phosphorylated on S588 (pS588-TBC1D4) [11]. It also had high affinity for TBC1D1 phosphorylated on T596 [16, 17] and may recognise other TBC1D1 and TBC1D4 phosphosites. This review focuses primarily on results obtained using antibodies that identify specific TBC1D1 or TBC1D4 phosphosites, and on results acquired using anti-PAS after immunoprecipitating the sample with anti-TBC1D1 or anti-TBC1D4 (PAS-TBC1D1 or PAS-TBC1D4). Unless otherwise specified, results in human muscle refer to the vastus lateralis.

TBC1D1 and TBCD4: muscle fibre type

TBC1D4 protein abundance in mice was about tenfold greater in the soleus muscle vs extensor digitorum longus muscle (EDL) or tibialis anterior muscle (TA), and abundance of TBC1D1 in TA exceeded that in EDL (by approximately threefold) and soleus (by approximately tenfold) [18]. Mouse soleus was enriched with fibres expressing myosin heavy chain type I or IIa isoforms, with lower type IIb and IIx expression, whereas mouse TA and EDL were enriched with fibres expressing myosin heavy chain type IIb or IIx isoforms and lacked detectable type I or IIa expression. In contrast, TBC1D4 abundance did not differ among multiple rat muscles with divergent fibre type profiles, including the soleus (primarily type I), EDL and TA (primarily IIb and IIx) [19]. TBC1D1 abundance did not differ in rat soleus, EDL or TA. Significant differences were undetectable for TBC1D1 or TBC1D4 abundance in human gastrocnemius, vastus lateralis and soleus [20]. Striking differences in TBC1D1 and TBC1D4 abundance found among mouse muscles have not been observed for rats or humans. Determination of fibre type, TBC1D1 and TBC1D4 abundance in individual muscle fibres would provide insights that cannot be discerned using tissue-level analysis.

TBC1D4: insulin-stimulated GLUT4 translocation in adipocytes

Control of GLUT4 translocation by insulin requires the binding of insulin to its receptor, leading to tyrosine phosphorylation of the receptor and then of IRSs, promoting IRS engagement with phosphatidylinositol 3-kinase, facilitating phosphorylation (T308 and S473) and activation of Akt, a serine/threonine (S/T) kinase. A link between Akt and GLUT4 translocation was revealed by Gustav Lienhard’s group [11, 14], which discovered that insulin-mediated TBC1D4 phosphorylation is important for GLUT4 translocation in 3T3-L1 adipocytes. TBC1D4 includes a Rab-GAP domain, two phosphotyrosine-binding (PTB) domains, a calmodulin-binding domain (CBD) and five canonical Akt phosphomotifs (Fig. 1).
Fig. 1

Schematic diagrams illustrating some of the prominent structural features of TBC1D1 and TBC1D4 (Akt substrate of 160 kDa, AS160). Each protein includes a Rab-GAP domain, two PTB domains and a CBD. The potential phosphomotifs that are depicted for each protein are designated by the amino acid numbers for the respective human proteins, and each of these sites can be phosphorylated in response to insulin and/or exercise in skeletal muscle

Sano et al [14] reported that insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes was diminished by overexpression of TBC1D4 with a mutation (serine or threonine to alanine) preventing phosphorylation of either S588 or T642 (with the T642A mutation having a greater inhibitory effect than the S588A mutation), and simultaneous S588A/T642A mutations had a greater inhibitory effect than either single mutation. Simultaneous mutation to prevent phosphorylation on six phosphomotifs (S318A, S341A, S588A, T642A, S570A and S751A) caused little further inhibition. Analysis of adipocytes expressing a mutation that eliminated Rab-GAP activity (R973K mutant), independently or together with four Akt phosphomotif mutations (S318A, S588A, T642A, S751A; 4P mutant), revealed that site-selective phosphorylation by TBC1D4 is essential for insulin-stimulated GLUT4 translocation only if there is an active Rab-GAP domain. They proposed that insulin-stimulated phosphorylation of TBC1D4 by Akt on selected phosphomotifs leads to the attenuation of TBC1D4’s activation of Rab-GTPase proteins associated with GLUT4 vesicles, favouring Rab-GTP accumulation and GLUT4 vesicle exocytosis. It was subsequently demonstrated that 14-3-3 proteins bind with high affinity to phosphorylated T642 and with low affinity to phosphorylated S341 [21], and 14-3-3 binding to TBC1D4 may be important for insulin-stimulated GLUT4 translocation [22, 23].

There are more than 60 predicted members of the Rab-GTPases in the human genome. In a cell-free assay, The GAP domain of TBC1D4 showed activity against Rab2A, -8A, -10 and -14 [24]. In 3T3-L1 adipocytes, Rab10 was crucial for insulin-stimulated GLUT4 translocation [25], but in L6 myocytes, Rab8A and Rab13 (but not Rab10) were implicated as TBC1D4 targets required for insulin-stimulated GLUT4 translocation [26]. There are no known specific Rab-GTPases targeted by TBC1D4 in human myocytes or muscle tissue from any species.

There is controversy regarding which of the specific aspects of GLUT4 vesicle traffic are influenced by TBC1D4 [27, 28, 29]. One model proposes that TBC1D4 modulates GLUT4 vesicle release from retention without altering vesicle tethering, docking or fusion with cell surface membranes [30]. Another model proposes that after releasing GLUT4 vesicles from retention secondary to TBC1D4 phosphorylation and 14-3-3 binding, the N-terminal PTB domain of TBC1D4 facilitates GLUT4 vesicle docking/fusion with the plasma membrane [31]. Both models are based on the findings of experiments on adipocytes. Neither model has been assessed in muscle.

TBC1D4: insulin-stimulated glucose transport in muscle

Overexpression of wild-type (WT) TBC1D4 did not alter insulin-stimulated GLUT4 translocation in L6 myocytes, but expressing the 4P-TBC1D4 phosphomutant substantially reduced insulin-stimulated GLUT4 translocation [32, 33]. Expressing 4P-TBC1D4 in L6 myocytes inhibited insulin-stimulated GLUT4 translocation, and expression of TBC1D4 with both R973K and 4P mutations relieved this inhibition, demonstrating that an active GAP domain is essential for inhibition of insulin-stimulated GLUT4 translocation by TBC1D4 in myocytes. Insulin-stimulated mouse TA overexpressing 4P-TBC1D4 had ~50% lower in vivo glucose uptake vs controls [34]. Mice with a T649A-TBC1D4 knockin mutation (preventing phosphorylation at mouse T649-TBC1D4, equivalent to human T642-TBC1D4) had ~20% lower glucose uptake by the TA and quadriceps during a euglycaemic–hyperinsulinaemic clamp vs controls [35]. Furthermore, insulin-stimulated glucose uptake by and cell surface GLUT4 content of isolated soleus were lower for T649A-TBC1D4 knockin vs WT mice. Results for Akt2-null mice revealed that Akt2 is essential for increased glucose uptake by muscles in the presence of physiological insulin concentrations [36, 37], and Akt2 is crucial for muscle insulin-stimulated pT642-TBC1D4 [38]. Compelling evidence links Akt2-dependent pT642-TBC1D4 with insulin-regulated glucose uptake in muscle. Based on 3T3-L1 adipocyte results [14], it can reasonably be predicted that S588-TBC1D4 may regulate muscle glucose uptake, but a point mutation of S588-TBC1D4 has not been studied in muscle.

TBC1D1: insulin-stimulated glucose transport by muscle

TBC1D1 and TBC1D4 share 50% identity, and both include a GAP domain (79% identity), two PTB domains and a CBD (Fig. 1). TBC1D1 and TBC1D4 are highly conserved, with the mouse protein sharing ~90% identity with the respective human protein [16]. TBC1D4 has multiple canonical Akt phosphorylation motifs, whereas TBC1D1 only has one (T596-TBC1D1, which corresponds to T642-TBC1D4) [39].

Insulin-stimulated glucose transport has been studied in adipocytes with TBC1D1 overexpression, but because endogenous TBC1D1 expression is very low in white adipose tissue, using muscle to study the function of TBC1D1 is more straight-forward. Insulin-stimulation increases pT596-TBC1D1 in human [40] and mouse [41, 42] muscles. Insulin increases pT596-TBC1D1, but not pS237-TBC1D1 in L6 myocytes [17]. In C2C12 myocytes, insulin increased phosphorylation of multiple TBC1D1-phosphosites (including S235, T505, S507, S527 and S627) identified by mass spectrometry [43]. Although pS237-TBC1D1 and pT596-TBC1D1 are 14-3-3 binding sites, insulin did not increase the ability of TBC1D1 to bind 14-3-3 [17]. Insulin increased pS235-TBC1D1 and binding of the adaptor protein containing PH domain, PTB domain and leucine zipper motif 2 (APPL2) to TBC1D1 in myocytes and mouse EDL [44]. In myocytes overexpressing APPL2, the binding of TBC1D1 to APPL2 required pS235-TBC1D1 and attenuated insulin-stimulated pT596-TBC1D1 and glucose uptake [44]. Cells expressing a T596D-TBC1D1 phospho-mimic mutant were protected from APPL2 overexpression-induced insulin resistance. Experiments with Akt2-null mice revealed that Akt2 is essential for the insulin-mediated increase of pT596-TBC1D1 in TA [42]. S237-TBC1D1 did not become phosphorylated in insulin-stimulated human [40], mouse [41] or rat [45] muscle. In vivo insulin-stimulated glucose uptake by mouse TA was unaltered by overexpression of WT TBC1D1 or mutated TBC1D1 (S237A, T505A, T596A and S627A) [46]. Unlike TBC1D4, the influence of TBC1D1 on specific steps of insulin-stimulated GLUT4 trafficking has not been reported. The creation of mice with a T596A-TBC1D1 knockin mutation would help clarify the role of site-specific phosphorylation of TBC1D1 in muscle insulin-stimulated glucose transport.

TBC1D4: exercise-stimulated glucose transport in muscle

Studying contractile activity by ex vivo muscle enables the direct effects of contraction to be studied in isolation (from systemic consequences of exercise, such as blood-flow, hormones, etc.), as well as precise tension measurement and exact hormone or drug doses. Contractile activity by isolated rat epitrochlearis led to greater PAS-TBC1D4 [12] and pT642-TBC1D4 [45]. However, it is also crucial to understand the more complex effects of in vivo exercise. PAS-TBC1D4 [47], pT642-TBC1D4 and pS588-TBC1D4 [45, 48, 49, 50] were increased in rat epitrochlearis both immediately and 3–4 h after exercise. Endurance exercise also increases TBC1D4 phosphorylation in human muscle. Cycling (67% peak oxygen consumption [\( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)]) caused increased PAS-TBC1D4 at 60 and 90 min, but not at 1, 10 or 30 min [51]. PAS-TBC1D4 increased immediately and 3 h after exercise (60 min cycling, 60% \( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)), whereas the 14-3-3 binding capacity of TBC1D4 increased only immediately post exercise [52]. Cycling (20 min, 77% \( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)) increased pS711-TBC1D4 [53], but PAS-TBC1D4 was lower after the same protocol [51]. One-legged exercise (60 min, 80% peak work rate) caused increased TBC1D4 phosphorylation on S341, S588, T642, S704, and S751, but not on S318 or S666 [54]. Cycling (30 min, 70% maximal oxygen consumption) induced greater pS711-TBC1D4 in obese individuals [55]. In summary, endurance exercise can elevate TBC1D4 phosphorylation on multiple sites in rat and human muscles.

TBC1D4 phosphorylation has also been assessed in human muscle after strength-type or sprint exercise. PAS-TBC1D4 was unaltered [56] or reduced immediately after strength-type exercise [57], but elevated at 1, 2 [58] or 24 h [56] exercise. PAS-TBC1D4 was unaltered immediately after sprint cycling (30 or 120 s) [51]. Furthermore, sprint cycling (30 s) did not elevate pT642-TBC1D4 immediately, or at 30 min or 4 h post exercise, but values were increased at 2 h post exercise [59]. Immediately after longer duration interval cycling (4 × 4 min), pT642-TBC1D4 exceeded resting values for obese, non-diabetic men [60]. The mechanisms and consequences of increased TBC1D4 phosphorylation often observed after sprint or strength-type exercise are uncertain.

TBC1D4 phosphorylation has also been studied in mice after contractions (in situ or ex vivo) or in vivo exercise. Mice performing treadmill exercise (30 min) had greater gastrocnemius levels of pT642-TBC1D4 [38]. In contrast, 60 min of treadmill exercise did not alter TBC1D4 phosphorylation in mouse EDL at S588, T642, S704 or S751, and caused decreased EDL pS341-TBC1D4 [54]. In the soleus, there were no exercise effects on pS588-TBC1D4 or pS704-TBC1D4 concomitant with reduced pS341-TBC1D4, pT642-TBC1D4 and pS751-TBC1D4 [54]. Ex vivo contraction of mouse soleus caused greater pS704-TBC1D4 [54], unaltered pS588-TBC1D4 and reduced pT642-TBC1D4 and pS751-TBC1D4 [54]. Mass spectrometric analysis of mouse gastrocnemius immediately after in situ contractions revealed greater TBC1D4 phosphorylation (S269, S588, S591, S609, T642 and S781) [53]. In situ contraction by mouse gastrocnemius or TA elevated pS588-TBC1D4, reduced pT642-TBC1D4 and did not alter S318, S341 or S751 phosphorylation [61]. Disparate results in mice vs humans and rats may reflect species differences and/or other experimental differences (e.g. muscles studied, exercise/contraction protocols).

In contrast to the strong evidence linking TBC1D4 phosphorylation to insulin-stimulated glucose transport, the evidence is less convincing for the role of TBC1D4 role in contraction/exercise-stimulated glucose transport. In isolated rat epitrochlearis, a phosphatidylinositol 3-kinase (PI3K) inhibitor (wortmannin) prevented contraction-stimulated PAS-TBC1D4 without attenuating contraction-stimulated glucose transport, and an AMPK-inhibitor (compound C) reduced contraction-stimulated glucose transport without altering PAS-TBC1D4 [12, 62]. Direct evidence against an essential role for pT642-TBC1D4 is provided by the finding that contraction-stimulated glucose uptake in mouse EDL (in situ or ex vivo contractions) was not different for T649A knockin vs WT mice [61]. The absence of contraction-stimulated pT649-TBC1D4 in gastrocnemius and TA from WT mice also argues against pT649-TBC1D4 being required for contraction-stimulated glucose uptake. T649A knockin mice are insulin resistant [35], suggesting that pT649-TBC1D4 is more important for insulin-stimulated vs contraction-stimulated glucose uptake. Electroporation of mouse TA with WT TBC1D4 reduced contraction-stimulated glucose uptake, and there was a further ~20–25% deficit of contraction-stimulated glucose uptake for muscles transfected with the 4P-TBC1D4 mutant, suggesting the TBC1D4 phosphorylation may influence contraction-stimulated glucose uptake [34, 63, 64]. The explanation for the differing results of the 4P-TBC1D4 model vs the T649A knockin model on contraction-stimulated glucose uptake is uncertain, but may relate to the six- to eightfold overexpression of TBC1D4 in the 4P-TBC1D4 model, and/or the mutations of three additional phosphomotifs (including S588) in 4P-TBC1D4. Unexpectedly, muscles electroporated with R/K-TBC1D4 or R/K-4P-TBC1D4 mutants had greater contraction-stimulated glucose uptake vs empty vector controls. It is unclear why overexpression of WT TBC1D4, R/K-TBC1D4 and R/K-4P-TBC1D4 influenced contraction-stimulated glucose uptake, but these constructs did not alter insulin-stimulated glucose uptake in mouse TA [34]. Perhaps contraction/exercise and insulin regulate different subcellular pools of TBC1D4.

In human muscle, exercise can increase phosphorylation of S704-TBC1D4 (a 5′ AMP-activated kinase [AMPK] consensus phosphomotif) [53]. Contraction-stimulated glucose uptake was not different in mouse TA that was electroporated with WT TBC1D4 vs muscle electroporated with TBC1D4 mutated to prevent phosphorylation at this site [53]. These results argue against S704 being essential for contraction-stimulated glucose uptake.

Even if TBC1D4 phosphorylation is not a major regulator of contraction-stimulated glucose uptake, it is important to determine which S/T kinase(s) participate in exercise/contraction-induced changes in TBC1D4 phosphorylation because TBC1D4 phosphorylation may influence other cellular functions. Geraghty et al [21] evaluated the ability of purified S/T kinases (Akt, AMPK, serum- and glucocorticoid-responsive kinase 1 [SGK1] and 90 kDa ribosomal S6 kinase 1 [RSK1]) to phosphorylate TBC1D4 in a cell-free assay. Each of these S/T kinases strongly increased pS588-TBC1D4, and all but AMPK strongly increased pT642-TBC1D4 (AMPK weakly increased pT642-TBC1D4). The binding of TBC1D4 to 14-3-3 in an overlay assay was strongly increased by purified Akt, SGK1 or RSK1, and was increased to a much smaller extent by AMPK. In cell-free assays, recombinant AMPK (but not Akt1, Akt2 or protein kinase Cζ) increased pS704-TBC1D4 [53], and recombinant Ca2+/calmodulin-dependent protein kinase kinase α (CaMKKα) increased pS588-TBC1D4, but not pT642-TBC1D4 [65]. Cell-free experiments are informative, but additional approaches are essential to elucidate the physiological regulation of TBC1D4 phosphorylation.

Of the five S/T kinases known to increase TBC1D4 phosphorylation in cell-free assays, AMPK has been the most frequently studied to elucidate exercise/contraction effects. The AMPK-activator 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) induced greater PAS-TBC1D4 in rat epitrochlearis, suggesting that AMPK phosphorylated TBC1D4 [12]. Many studies have demonstrated that muscle AMPK is activated immediately after endurance exercise or ex vivo contractions [2, 3, 4]. Immediately after 60 min of one-legged exercise by humans, TBC1D4 phosphorylation (S341, S588, T642 and S704) was increased, but only pS341-TBC1D4 and pS704-TBC1D4 were significantly correlated with α2/β2/γ1 AMPK activity [54]. Increased pT642-TBC1D4 in the TA after in situ contractions was blunted in transgenic mice with muscle-specific expression of inactive AMPK α2 vs WT mice [66].

Some [12, 67, 68, 69], but not all [70, 71, 72], studies have demonstrated that Akt is activated immediately after electrically stimulated contractions. Increased Akt activation has been observed in muscles immediately after in vivo endurance exercise without added insulin in some [68, 73], but not all [74, 75, 76], studies. There was no attenuation of pT642-TBC1D4 in EDL from Akt2-null mice after in situ contractions [38].

Little is known about the possible roles of SGK1, RSK1 or CaMKKα in exercise-stimulated TBC1D4 phosphorylation. Endurance exercise induced greater pT642-TBC1D4, pS588-TBC1D1 and pT173-AMPK in rat epitrochlearis without increased phosphorylation of regulatory sites on Akt, SGK1 or RSK1 [48].

Increased cytosolic calcium is essential for muscle contraction and a potential trigger for contraction-stimulated glucose transport. Although overexpression of TBC1D4 with a CBD mutation preventing TBC1D4 binding to calmodulin did not alter insulin-stimulated glucose uptake, this mutation modestly reduced contraction-stimulated glucose uptake by mouse TA [64]. Overexpressing TBC1D4 with simultaneous CBD and 4P mutations in mouse TA did not cause a greater reduction in contraction-stimulated glucose uptake than with either mutation alone [64].

TBC1D1: exercise/contraction-stimulated glucose transport in muscle

Many studies have evaluated the effect of contractions on TBC1D1 phosphorylation in rodents. Contraction increased PAS-TBC1D1 in mouse TA [18] and rat epitrochlearis [62]. In isolated rat epitrochlearis, contraction-stimulated PAS-TBC1D1 was eliminated by the AMPK-inhibitor compound C with attenuation of pT172-AMPK and glucose transport [62]. Contraction elevated pS237-TBC1D1 (an AMPK consensus motif) in rat epitrochlearis [45] and increased pS237-TBC1D1 and pT596-TBC1D1 in the EDL of WT mice, but phosphorylation was not increased on either site in AMPKα2 kinase-dead mice [41]. Contraction-stimulated EDL glucose uptake in AMPKα2 kinase-dead mice was lower than that in WT mice [77]. Contraction increased the binding capacity of TBC1D1 for 14-3-3 in EDL in WT mice, but not in AMPKα2 kinase-dead mice [41]. Contraction by WT mouse TA increased pS237-TBC1D1 [42, 61] and pS666-TBC1D1, but did not alter pT596-TBC1D1 [42]. Contraction effects on these sites were markedly lower for AMPKα2 inactive transgenic vs WT mice [42], whereas contraction effects were similar for Akt2-null vs WT mice [41]. In isolated EDL from WT mice, contraction induced increases in pS237-TBC1D1, pT596-TBC1D1, pS660-TBC1D1 and pS700-TBC1D1, and these effects were either eliminated or almost eliminated in AMPKα2-null mice [54]. Contraction-stimulated glucose uptake in the EDL was unaffected for AMPKα1-null mice vs WT mice [78]. However, AMPKα2-null mice have a compensatory increase in AMPKα1 expression. Muscle-specific β1β2-null mice have undetectable muscle AMPK activity accompanied by markedly reduced contraction-stimulated (isolated EDL and soleus) and in vivo treadmill exercise-stimulated (gastrocnemius and soleus) glucose uptake [79]. Contraction-stimulated PAS-TBC1D1 was also nearly eliminated in isolated EDL of β1β2-null mice. Treadmill exercise (60 min) increased p237-TBC1D1, pT596-TBC1D1, pS660-TBC1D1 and pS700-TBC1D1 in the EDL, but not the soleus, and pS596-TBC1D1 was unaltered by exercise in EDL or soleus [54]. This treadmill protocol induced an increase in pT172-AMPK in the EDL, but not the soleus, and exercise decreased pS473-Akt and pT308-Akt in both muscles. In vivo contraction-stimulated glucose uptake was ~30–45% lower in mouse TA overexpressing TBC1D1 with a mutation preventing phosphorylation on four phosphomotifs (including S237) [42, 46]. Contraction or exercise usually elevates pS237-TBC1D1 of rodent muscle, and this increase appears to be secondary to greater AMPK activity and linked to greater contraction/exercise-stimulated glucose uptake.

Several studies have assessed the effect of exercise on TBC1D1 phosphorylation in human muscle. Increased pS237-TBC1D1, but not pT596-TBC1D1, was observed immediately after high-intensity (30 s or 2 min) or moderate-intensity (20 min, 77% \( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)) cycling [80]. These protocols also increased the binding capacity of TBC1D1 for 14-3-3. Immediately after cycling (30 min, 70% \( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)), pS237-TBC1D1 was increased, but pS596-TBC1D1 was not, in muscles of obese people [55]. Immediately after one-legged exercise (60 min), there were increases in pS237-TBC1D1 and pT596-TBC1D1 that correlated with exercise-induced increases in α2/β2/γ3 AMPK activity [54]. Exercise also produced elevations in pS660-TBC1D1 and pS700-TBC1D1, but not in the binding capacity of TBC1D1 for 14-3-3. S237-TBC1D1 and T596-TBC1D1 phosphorylation were increased at 30 min after cycling (60 min, 65% \( \dot{V}{\mathrm{O}}_{2\mathrm{peak}} \)) [81]. As in rodents, human exercise consistently increases pS237-TBC1D1, but does not always increase pT596-TBC1D1. Increased S237-TBC1D1 phosphorylation is a promising candidate in the regulation of exercise-dependent glucose uptake by human muscle.

TBC1D1 and TBC1D4: insulin sensitivity after acute exercise

Several hours after one exercise session by healthy individuals, insulin-stimulated glucose transport is increased secondary to elevated GLUT4 translocation without increased GLUT4 expression or enhanced proximal insulin signalling, including insulin receptor binding, IRS1 tyrosine phosphorylation, IRS1-associated PI3K activity and Akt activity [8, 49, 50, 75, 82, 83, 84, 85]. Despite unaltered proximal signalling, acute exercise can increase TBC1D4 phosphorylation and glucose uptake in muscle stimulated with a physiological insulin dose (Fig. 2) [40, 45, 47, 48, 49, 50, 86]. Isolated rat epitrochlearis incubated with a physiological insulin concentration at 3–4 h post exercise had increased PAS-TBC1D4 [47], pS588-TBC1D4 [48, 50] and pT642-TBC1D4 [45, 48, 49, 50]. Increased insulin-stimulated glucose transport tracked closely with changes in pT642-TBC1D4: both persisted for 27 h post exercise in muscles from rats that were not fed chow after exercise, but consuming chow for 3 h post exercise reversed these exercise effects [49]. Wojtaszewski and colleagues probed the relationship between TBC1D4 phosphorylation and insulin-stimulated glucose uptake in healthy humans after one-legged exercise for 60 min [40, 86]. Muscle glucose uptake was measured for the non-exercising and exercising leg during a clamp (with a physiological insulin concentration). Pehmøller et al [40] reported that insulin-stimulated muscle from the exercised vs non-exercised leg had greater TBC1D4 phosphorylation on S318, S341, S588, T642, S704 and S751, whereas Treebak et al [86] reported increased TBC1D4 phosphorylation on S318, S341 and S751, with a non-significant trend for greater pS588-TBC1D4, and unaltered pT642-TBC1D4 and pS666-TBC1D4. The binding affinity of TBC1D4 for 14-3-3 (measured by overlay assay) was unaltered by exercise in both studies. High-intensity interval cycling (4 × 4 min; 90–95% heart-ratepeak) induced greater glucose infusion during a euglycaemic–hyperinsulinaemic clamp with a physiological insulin dose, but did not significantly increase muscle pT642-TBC1D4 determined ~3 h post exercise [60]. In isolated mouse plantaris incubated with a supraphysiological insulin dose 24 h after treadmill exercise (90 min duration), there was increased insulin-stimulated glucose uptake with no exercise-effect on pT642-TBC1D4 [87]. Most, but not all, studies in healthy rats or humans using a physiological insulin dose after acute exercise have reported increased TBC1D4 phosphorylation.
Fig. 2

Effect of acute exercise on subsequent site-specific TBC1D1 and TBC1D4 phosphorylation in normal muscle in response to a physiological insulin dose. This figure depicts the results of published experiments for acute endurance exercise that resulted in a subsequent elevation of insulin-stimulated glucose uptake with a physiological insulin dose in non-insulin resistant, non-diabetic rats or humans [40, 45, 47, 48, 49, 50, 86]. The phosphomotifs shown in red font did not have an exercise-induced increase in insulin-stimulated phosphorylation compared with unexercised control muscle. The phosphomotifs shown in green font were found in most of the published studies to have an exercise-induced increase in insulin-stimulated phosphorylation vs unexercised controls. The numbers in parentheses below each phosphomotif represent the ratio of the number of publications with a significant exercise-induced increase in insulin-stimulated phosphorylation and the total number of publications making the relevant exercise comparison

Prior exercise does not elevate the phosphorylation of all Akt substrates in insulin-stimulated muscles. There was no increase in PAS-TBC1D1 in insulin-stimulated rat epitrochlearis at 3 or 27 h post exercise [49]. Neither pT596-TBC1D1 nor pS237-TBC1D1 differed for exercised vs non-exercised insulin-stimulated muscles sampled at ~5–7 h post exercise [40]. There were also no exercise effects on insulin-stimulated pS21/9-glycogen synthase kinase 3 (GSK3) in rat epitrochlearis at 3–4 h post exercise [47], or on insulin-stimulated pS21-GSK3 in human muscle at ~4–6 h post exercise [83]. The effect of exercise on pTBC1D4 is at least somewhat specific, although it will be important to evaluate additional Akt substrates. Apparent uncoupling between the extent of Akt activation and the levels of phosphorylation of various substrates (and even individual phosphosites on one substrate) is not unique to the post-exercise condition [85, 86, 87].

What are the mechanisms that account for greater TBC1D4 phosphorylation in insulin-stimulated muscle several hours post exercise without greater Akt activity? Perhaps prior exercise leads to greater activation of other S/T kinases that can phosphorylate TBC1D4. However, insulin did not increase the phosphorylation of activation sites for AMPK, SGK1 or RSK1 in epitrochlearis from unexercised or exercised rats [48]. Protein phosphorylation represents the balance between kinases and phosphatases. It is uncertain which S/T protein phosphatase(s) dephosphorylates TBC1D4, but protein phosphatase 2A (PP2A) is abundantly expressed by muscle. However, exercise did not alter Y307 phosphorylation or L309 methylation of the catalytic subunit of PP2A [48].

Each of the direct determinants of protein phosphorylation (i.e. relevant kinase(s) and phosphatases and the substrate itself) might be regulated by interactions with other protein binding partners, various post-translational modifications, allosteric modulation and/or subcellular localisation (Fig. 3). Assuming Akt is the relevant kinase, conventional cell-free Akt enzyme assays do not address allosteric modulation or subcellular localisation, and immunoprecipitated Akt may not retain all endogenous protein binding partners or all post-translational modifications. The first step in understanding the role of S/T protein phosphatase(s) would be to identify the relevant enzymes that dephosphorylate TBC1D4. Several proteins have been identified as TBC1D4 binding partners, including 14-3-3, RuvB-like 2 (RUVBL2), receptor-interacting protein of 140 kDa (RIP140) and cytoplasmic linker protein-protein 59 kDa protein (ClipR-59) [22, 88, 89, 90, 91]. The effect of exercise on the capacity of TBC1D4 binding to 14-3-3 using an overlay assay has been reported [40, 86], but the direct binding of endogenous 14-3-3 (or any of these other proteins) to TBC1D4 has not been reported for insulin-stimulated muscle after acute exercise.
Fig. 3

Potential mechanisms for increased TBC1D4 phosphorylation in the absence of greater Akt activity in insulin-stimulated muscle after acute exercise. This figure depicts potential mechanisms that might account for acute exercise effects on TBC1D4 phosphorylation in insulin-stimulated muscle. One exercise session can increase the subsequent (~3–27 h post exercise) TBC1D4 phosphorylation in insulin-stimulated muscle without an increase in Akt activity determined by a conventional Akt enzyme activity assay (using immunoprecipitated Akt with an Akt peptide substrate) [49, 50]. Muscle TBC1D4 phosphorylation depends on the relevant kinase activity, the relevant S/T phosphatase activity and the properties of TBC1D4 that influence its susceptibility to the kinase and phosphatase. A conventional cell-free Akt assay cannot account for the in-cell co-localisation of TBC1D4 with Akt and S/T phosphatases. Enzyme activity of Akt and S/T phosphatases can be influenced by their binding to other proteins. TBC1D4 also binds to regulatory proteins that can influence its phosphorylation. Post-translational modifications (other than S/T phosphorylation) can regulate the activity of Akt and some S/T phosphatases, but it is currently unknown whether TBC1D4 has any post-translational modifications other than S/T phosphorylation

Improvements in insulin sensitivity can be observed in insulin-resistant individuals after acute exercise, but few studies have probed the mechanism that underlies these improvements. Castorena et al [50] found that, 3–4 h after endurance exercise, insulin-stimulated glucose uptake was elevated in isolated epitrochlearis from normal rats (eating a low-fat diet, LFD) and insulin-resistant rats eating a high-fat diet (HFD). Insulin-stimulated Akt activity was unaltered in both diet-groups at 3 h post exercise. In insulin-stimulated muscles from LFD-rats at 3 h post exercise, the levels of pS588-TBC1D4 and pT642-TBC1D4 exceeded those in diet-matched sedentary controls. At 3 h post exercise, the insulin-resistant HFD rats had increased pS588-TBC1D4 (but not pT642-TBC1D4) in insulin-stimulated muscles vs diet-matched sedentary controls. After exercise, pS588-TBC1D4 and pT642-TBC1D4 in insulin-stimulated muscles from HFD animals equalled the values in sedentary LFD controls. However, exercise failed to elevate insulin-stimulated glucose uptake, pS588-TBC1D4 or pT642-TBC1D4 in the HFD group to values as great as those in the post-exercise LFD-group. In both diet-groups, insulin-stimulated glucose uptake consistently tracked with TBC1D4 phosphorylation post exercise.

Pehmøller et al [40] induced insulin resistance in healthy humans by infusing intralipid for 7 h and studied the same individuals in a control saline infusion condition. In both trials, participants performed 60 min of one-legged exercise ~5.5 h prior to a clamp with a physiological insulin concentration. Exercised vs non-exercised legs had greater glucose uptake and TBC1D4 phosphorylation (S318, S341, S588, T642, S704 and S751) in both trials, without exercise effects on proximal insulin signalling (IRS-1–PI3K or Akt phosphorylation). Similar to the results for insulin-resistant rats [50], exercise improved insulin-stimulated glucose uptake by insulin-resistant muscles levels equivalent to normal, non-exercised values [40]. Also similar to the findings in insulin-resistant rats, insulin-stimulated glucose disposal was lower in the post-exercise lipid-infusion trial compared with the post-exercise saline-infusion trial.

In HFD-fed streptozotocin-induced diabetic rats, ~8–16 h post exercise, glucose transport and pS473-Akt were increased in isolated soleus with a supraphysiological insulin dose concomitant with a non-significant trend for increased pT642-TBC1D4 [92]. In old (24 months) rats, there was increased insulin-stimulated glucose uptake by isolated epitrochlearis and soleus with two submaximally effective insulin doses at 3 h post exercise [93]. Exercise increased pT642-TBC1D4 in the epitrochlearis (but not soleus) with only one of the submaximally effective insulin doses studied, and exercise did not significantly increase pS588-TBC1D4 in either muscle, regardless of insulin dose [93]. Acute exercise can cause insulin-resistant muscles to increase their insulin-stimulated glucose uptake to values similar to those in normal, non-exercised controls, and greater TBC1D4 phosphorylation may be related to improved insulin sensitivity post exercise.

TBC1D4: chronic exercise effects on insulin sensitivity

One exercise session can enhance insulin sensitivity, but regularly performed exercise for several days, weeks or months may produce further improvements [10]. Endurance training (10 weeks) increased glucose disposal during a euglycaemic–hyperinsulinaemic clamp performed 48 h after the final exercise bout in obese men with or without type 2 diabetes [94]. Training increased TBC1D4 phosphorylation (S318, S341, S588, T642 and S751) in insulin-stimulated muscles from the diabetic group, despite there being no training effects on pS473-Akt or pT308-Akt. In the non-diabetic group, with greater TBC1D4 phosphorylation (S341, T642 and S751), pS473-Akt was increased but pT308-Akt was not. Endurance training (12 weeks) by young (24 years) or old (69 years) non-diabetic humans resulted in increased glucose disposal during a clamp (~40 h after final training session) in both age groups [88]. The older group was modestly insulin-resistant compared with the young group. Endurance training increased pS588-TBC1D4 in both groups, increased pT642-TBC1D4 only in the older group, and did not alter pS666-TBC1D4 in either group. Both age groups were also studied following strength training (12 weeks), and glucose disposal during a clamp (~40 h after final training session) increased in both age groups. Strength training increased pT642-TBC1D4 in both age groups, increased pS666-TBC1D4 in the older group only and did not alter pS588-TBC1D4 regardless of age. In both insulin-resistant, non-diabetic HFD-fed rats and streptozotocin-induced diabetic HFD-fed rats, endurance training (8 weeks) increased glucose transport by isolated soleus incubated with a supraphysiological insulin dose (~24–36 h after final training bout) [92]. In both groups, training increased pT642-TBC1D4 in muscles incubated with supraphysiological insulin [92]. Endurance training (4 weeks) increased glucose uptake without increasing pT642-TBC1D4 in perfused hindlimb muscles of rats made insulin resistant by a cafeteria diet (24 h after final training session) [95]. To the best of my knowledge, there are no published reports of training effects on insulin-stimulated TBC1D1 phosphorylation. In some experimental conditions, exercise training induces greater site-specific phosphorylation of TBC1D4 in insulin-stimulated muscle, which, together with other exercise training effects (e.g. increased GLUT4 protein expression [4]) may contribute to improved insulin sensitivity.

TBC1D1-null and TBC1D4-null mice

Mice with a naturally occurring loss-of-function mutation of the Tbc1d1 gene had reduced GLUT4 abundance and ex vivo insulin-stimulated glucose uptake in EDL (high TBC1D1 expression in WT mice), but not in soleus (low TBC1D1 expression in WT mice) [96, 97]. Soleus, gastrocnemius and diaphragm glucose uptake during a euglycaemic–hyperinsulinaemic clamp was unaltered for TBC1D1-deficient mice, but, paradoxically, EDL and TA glucose uptake were greater for TBC1D1-deficient mice [97]. Conventional TBC1D1-null mice had reduced GLUT4 abundance and insulin-stimulated glucose uptake by isolated EDL [98]. TBC1D4 abundance (soleus and EDL), glucose tolerance and insulin tolerance were unaltered in TBC1D1-null mice.

Whole body TBC1D4-null mice had lower GLUT4 in muscles with high TBC1D4 expression in WT mice (soleus, gastrocnemius and vastus lateralis), but not in muscles with low TBC1D4 expression in WT mice (EDL and TA) [99, 100]. Glucose uptake for TBC1D4-null mice was lower for isolated soleus, but not EDL [100]. Soleus Glut4 mRNA was not lower in TBC1D4-null vs WT mice. Muscle TBC1D1 abundance was similar between genotypes. TBC1D4-null mice had reduced soleus and vastus lateralis glucose uptake during a euglycaemic–hyperinsulinaemic clamp [100]. Both TBC1D1 and TBC1D4 deficiency are characterised by muscle-specific insulin resistance that appears to be related to lower GLUT4 abundance. Perhaps in the absence of TBC1D1 or TBC1D4, GLUT4 trafficking rates increase, leading to accelerated GLUT4 protein degradation and lower GLUT4 abundance. It is important to note that muscle-specific knockouts of TBC1D1 and TBC1D4 have not yet been evaluated.

Summary of current knowledge and areas for future study

TBC1D1 and TBC1D4 have distinctive roles in regulating muscle glucose transport. Akt2-dependent pT642-TBC1D4 is required for the full effect of insulin on glucose transport in muscle. However, the functional significance of insulin-stimulated increases in the phosphorylation of several other individual TBC1D4 sites (e.g. S588) has not been determined in muscle. Exercise/contraction-induced glucose uptake often coincides with greater TBC1D1 phosphorylation, but there is no information linking specific GLUT4 vesicle trafficking events (retention, tethering, docking, fusion) to TBC1D1-dependent, site-specific phosphorylation in cultured cells or exercised muscle. Greater phosphorylation of TBC1D4, but not TBC1D1, often tracks closely with the enhanced glucose transport in muscle stimulated with a physiological insulin dose after acute exercise. However, the mechanisms accounting for increased TBC1D4 phosphorylation and its causal importance for improved post-exercise insulin sensitivity remain to be established. Future research should focus on the subcellular localisation of TBC1D1 and TBC1D4 because localised subsets of each protein are likely to perform specific functions. In this context, it is interesting that research using 3T3-L1 adipocytes revealed that a phospholipid-binding region of the N-terminus PTB domain of TBC1D4 regulates the localisation of this protein at the plasma membrane and increases GLUT4 docking/fusion with the plasma membrane. Similar experiments are lacking for muscle. Finally, skeletal muscle highly expresses both TBC1D1 and TBC1D4, and elucidating the complementary and antagonistic interactions between these paralog proteins is needed to fully understand their biological roles, which are likely not limited to the regulation of glucose transport.

Notes

Funding

Research related to this topic is supported by grants to GDC from the National Institutes of Health (DK71771 and AG10026).

Duality of interest

The author declares that there is no duality of interest associated with this manuscript.

Contribution statement

GDC was the sole contributor to this paper.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Muscle Biology Laboratory, School of KinesiologyUniversity of MichiganAnn ArborUSA
  2. 2.Department of Molecular and Integrative PhysiologyUniversity of MichiganAnn ArborUSA
  3. 3.Institute of GerontologyUniversity of MichiganAnn ArborUSA

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