Diabetologia

, Volume 54, Issue 6, pp 1539–1542 | Cite as

Increased prevalence of proliferative retinopathy in patients with type 1 diabetes who are deficient in glucose-6-phosphate dehydrogenase

  • G. Cappai
  • M. Songini
  • A. Doria
  • J. D. Cavallerano
  • M. Lorenzi
Short Communication

Abstract

Aims/hypothesis

Impaired activity of the pentose phosphate pathway of glucose metabolism caused by hereditary deficiency of its key regulatory enzyme glucose-6-phosphate dehydrogenase (G6PD) has consequences that may worsen or attenuate the course of diabetic complications. Decreased availability of NADPH can predispose to oxidative stress and endothelial dysfunction, but can also limit the activity of the polyol pathway and cholesterol synthesis. Reduced availability of pentose phosphates for nucleic acid synthesis could impair cell proliferation. We sought to learn in which direction G6PD deficiency affects diabetic retinopathy.

Methods

We enrolled patients who were G6PD-deficient or -sufficient with type 1 diabetes of duration 15 years or longer for whom HbA1c records were available for at least the previous 3 years. Renal failure and smoking were exclusion criteria. For each participant seven standard field colour photographs were obtained of each eye, and retinopathy was graded in a masked fashion.

Results

The clinical characteristics of the 19 G6PD-deficient patients studied (age 42 ± 9 years, diabetes duration 24 ± 6 years, average HbA1c over 3 years 6.7 ± 0.8%) were similar to those of the 35 G6PD-sufficient patients. Almost 90% of patients in both groups had retinopathy; however, proliferative retinopathy was noted solely among G6PD-deficient patients (28%, p = 0.0036 vs G6PD-sufficient). The G6PD-deficient patients also showed a trend for increased frequency of microalbuminuria.

Conclusions/interpretation

The data suggest that G6PD deficiency accelerates the microvascular complications of diabetes, and that among the consequences of G6PD deficiency those that can enhance the damage caused by diabetes outweigh those that could be protective.

Keywords

Diabetic retinopathy Endothelial nitric oxide synthase G6PD deficiency Oxidative stress Polyol pathway Proliferative retinopathy Type 1 diabetes 

Abbreviations

EDIC

Epidemiology of Diabetes Interventions and Complications

eNOS

Endothelial nitric oxide synthase

ETDRS

Early Treatment Diabetic Retinopathy Study

G6PD

Glucose-6-phosphate dehydrogenase

NPDR

Non-proliferative diabetic retinopathy

PDR

Proliferative diabetic retinopathy

Introduction

Diabetic retinopathy remains a complication of diabetes even among individuals who have made a systematic and long-term attempt to optimise glycaemic control [1]. New approaches to lessen its development or progression are desirable. Genetic variations offer the opportunity to identify, in humans, factors that modulate the pathogenic process and may suggest targets for adjunct therapy.

Glucose-6-phosphate dehydrogenase (G6PD) is the key regulatory enzyme of the pentose phosphate pathway of glucose metabolism, which provides pentose phosphates for nucleic acid synthesis and NADPH for synthetic reactions and maintenance of the cellular redox status. G6PD deficiency is the most common enzymopathy in humans. It is a hereditary abnormality in which the activity or stability of the enzyme is greatly diminished. As the gene determining the structure of G6PD is on the X chromosome, inheritance is sex-linked with men expressing the full defect.

The consequences of G6PD deficiency could worsen, but also attenuate, the processes leading to diabetic microangiopathy. On the one hand, NADPH is required to regenerate reduced glutathione, and thus G6PD deficiency facilitates oxidative stress. The main clinical manifestation of G6PD deficiency is a haemolytic anaemia, occurring because the erythrocytes, which rely exclusively on the pentose phosphate pathway to generate NADPH, are insufficiently protected from oxidation in the presence of drugs, infections and consumption of broad beans (or fava beans, ‘favism’) [2]. G6PD deficiency could also contribute to diabetic vascular disease by magnifying endothelial dysfunction, as NADPH is a required cofactor for endothelial nitric oxide synthase (eNOS) [3]. On the other hand, the reduced cellular supply of NADPH could protect from mechanisms of diabetic tissue damage. By dampening the activity of aldose reductase, which preferentially uses NADPH as a cofactor to convert glucose to sorbitol, the limited NADPH supply could prevent polyol pathway activity. Decreased activity of the first step of the pathway has been observed in cultured fibroblasts from individuals with G6PD deficiency [4], supporting the concepts that the NADPH supply is decreased not only in erythrocytes but also in nucleated cells [5], and that the decreased supply may thus limit the contribution of excess polyol pathway activity to retinopathy and other complications of diabetes. G6PD deficiency could attenuate diabetic vascular disease also on account of decreased cholesterol synthesis and a favourable lipid profile [5], as NADPH is a cofactor for 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase. In view of the multiplicity of reactions affected by the products of the pentose phosphate pathway, the net effect of G6PD deficiency on a specific tissue or function is difficult to predict. The findings on cardiovascular mortality of Sardinian G6PD-deficient men illustrate the challenge: expected to be increased on account of the widely hypothesised role of oxidative stress in atherothrombotic disorders, cardiovascular mortality was instead found to be substantially reduced [2].

To learn experimentally whether and in which direction G6PD deficiency affects diabetic retinopathy, we took advantage of the epidemiology of type 1 diabetes and G6PD deficiency in the Italian island of Sardinia. Sardinia has one of the highest incidences of type 1 diabetes in the world [6], second only to Finland, and a very high prevalence of G6PD deficiency (present in 12–15% of the male population compared with 0.48% in continental Italy) [2]. In Sardinia, all variants of G6PD deficiency are due to the same mutation, which results in only 5–10% residual activity of the enzyme [7] and G6PD deficiency does not show an association with diabetes [8]. We could thus plan to compare the prevalence and severity of retinopathy in diabetic patients with a homogeneously severe degree of G6PD deficiency and in patients with the same duration of type 1 diabetes and the same glycaemic control but with normal G6PD activity.

Methods

The institutional review boards of the Schepens Eye Research Institute and the Joslin Diabetes Center in Boston, USA, and of the Azienda Ospedaliera G. Brotzu in Cagliari, Italy, approved the study. The central recruitment site was the Diabetes Center of the Ospedale San Michele in Cagliari (Azienda Ospedaliera G. Brotzu); additional referrals were received from local diabetes specialists. We recruited male patients, 18–55 years of age with type 1 diabetes of duration 15 years or longer, with or without G6PD deficiency. An essential criterion for inclusion in the study was the availability, through databases, of HbA1c records spanning at least 3 years. Exclusion criteria were retinal or systemic diseases other than diabetes, renal insufficiency (serum creatinine ≥ 132.6 μmol/l) and smoking. After the participants had given informed consent, we retrieved the sequential measurements of HbA1c over the preceding 3 years, as well as the measurements of serum creatinine, albumin excretion rate (overnight urine collections), and serum lipids obtained within the preceding 6 months. Erythrocyte G6PD activity was either available in the databases or measured for this study by the Clinical Laboratories of the San Michele Hospital. Blood pressure was measured at the enrolment visit.

Each participant underwent a complete eye examination. A photographer took Early Treatment Diabetic Retinopathy Study (ETDRS) seven standard field 35 mm stereoscopic colour photographs of each eye using a 35 mm colour slide film and a standard mydriatic fundus camera. An experienced grader (J.   D. Cavallerano), masked to all patient characteristics, graded the slides according to modified ETDRS protocol [9]. The retinopathy grade assigned to the individual patient was that for the eye with the more advanced level of retinopathy. Macular oedema was an independent notation.

The variables in the two groups of patients were compared using a two-tailed t test, except in the case of binary or categorical variables, for which the frequencies were compared by means of Fisher’s exact tests.

Results

Table 1 presents the characteristics of the patients studied and the comparisons between type 1 diabetic patients with and without G6PD deficiency. Age and duration of diabetes were almost identical. The HbA1c levels were, on average, 1% lower in the G6PD-deficient group (p = 0.0001 vs controls), consistent with the extent by which HbA1c is lower in G6PD-deficient vs G6PD-sufficient non-diabetic individuals [10], most likely reflecting increased erythrocyte turnover. The prevalence of any retinopathy approached 90% in both groups; however, PDR was present only among patients with G6PD deficiency (p = 0.0036 vs the control group). Four of the five cases were quiescent PDR treated with laser, and one was PDR without high-risk characteristics. Consistent with the increased prevalence of PDR, there was a tendency for an increased frequency of retinopathy grade 4 or higher in the G6PD-deficient group compared with the control group (39% vs 18%; p = 0.17). The G6PD-deficient group also showed a trend for increased prevalence of microalbuminuria, although the p value did not reach significance (Table 1).
Table 1

Characteristics of the diabetic patients studied and prevalence of complications

Characteristic

Type 1 diabetes

Type 1 diabetes, G6PD-deficient

p value

n = 35

n = 19

Age (years)

42.6 ± 8.5

42.0 ± 8.7

0.8

Male sex (%)

100

100

 

Diabetes duration (years)

24.0 ± 7.2

24.4 ± 6.0

0.8

G6PD activity (U/g Hb)a

13.5 ± 3.2

2.0 ± 0.6

<0.0001

HbA1c (%)b

7.8 ± 0.9

6.7 ± 0.8

0.0001

HbA1c measurements (n)c

8.8 ± 2.3

9.2 ± 2.1

0.5

Blood pressure, systolic (mmHg)

122 ± 10

126 ± 10

0.15

Blood pressure, diastolic (mmHg)

74 ± 7

77 ± 5

0.09

Albumin excretion rate (μg/min)d

8 (5–16)

16 (7–40)

0.14

Microalbuminuria, n (%)e

6 (17.4)

7 (36.8)

0.18

Serum creatinine (μmol/l)

79.6 ± 8.8

79.6 ± 8.8

0.9

Total cholesterol (mmol/l)

4.7 ± 0.8

5.2 ± 1.3

0.26

HDL-cholesterol (mmol/l)

1.5 ± 0.3

1.7 ± 0.6

0.31

Intraocular pressure R (mmHg)

14.5 ± 1.9

14.1 ± 1.9

0.43

Intraocular pressure L (mmHg)

14.6 ± 1.8

13.9 ± 2.0

0.19

Retinopathy, any n (%)f

29 (88)

16 (89)

0.99

Retinopathy, proliferative

n (%)

0

5 (28)

0.0036

Macular oedema in at least one eye, n (%)g

12 (36)

5 (28)

0.76

Data are means ± SD or percentages, except as indicated

aReflects measurements performed by the same laboratory (G6PD kit, bcs Biotech, Cagliari, Italy) in 29 of the diabetic patients with normal G6PD and ten of the G6PD-deficient diabetic patients. Values for G6PD activity in the remaining patients were obtained by diverse methods, and the values for the G6PD-deficient patients were indicated as 0 U/g Hb. These data were not used in computations

bReflects measurements recorded over the preceding 3 years

cNumber of measurements available over the preceding 3 years for each patient

dPresented as median and interquartile range. Comparison between the two groups is based on log-transformed values to approximate the normal distribution

eMicroalbuminuria was considered present when the albumin excretion rate was 20 μg/min or greater

fRetinal photographs were available and graded in 33 of the diabetic patients with normal G6PD and 18 of the G6PD-deficient patients. Diabetic retinopathy was graded 0 if absent, 1 if questionable, 2 if mild NPDR, 3 if moderate NPDR, 4 if severe NPDR, 5 if very severe NPDR, 6 if PDR without high-risk characteristics, 7 if PDR with high-risk characteristics and 8 if quiescent PDR

gThe presence and degree of macular oedema was determined from review of the retinal photographs according to the ETDRS criteria. Macular oedema was defined as thickening of the retina within one disc diameter of the centre of the macula and/or hard exudates greater than or equal to standard photograph 3 in a standard 30-degree photographic field centred on the macula, with some hard exudates within one disc diameter of the centre of the macula

L, left; R, right

Discussion

By having no cases of PDR among 33 patients with 24 ± 7 years of diabetes, the population of type 1 diabetic patients with normal G6PD examined in this study compares very favourably with larger series of intensively treated patients. The prevalence of PDR in the DCCT- Epidemiology of Diabetes Interventions and Complications (EDIC) patients with a duration of diabetes (25 ± 5 years) and HbA1c levels similar to those of our patients was 4.4% in intensively treated patients from the prevention arm of the DCCT, and 13.2% in patients from the intervention arm [1]. In our G6PD-deficient patients with 24 ± 6 years of diabetes the frequency of PDR (28%) was much higher than in the control group and the DCCT-EDIC cohorts; the frequency of severe and very severe NPDR and of microalbuminuria also tended to be greater than in the control group. Glycaemic exposure could not be assessed precisely because the relationship of glycaemic levels to HbA1c is altered in G6PD-deficient patients [10], and longitudinal measurements of indices not based on erythrocytes were not available. However, we documented that the HbA1c levels of our G6PD-deficient patients were lower than those of G6PD-sufficient patients, and by the extent attributed in non-diabetic individuals to the erythrocyte abnormalities [10]. Drugs that block the renin–angiotensin system and may introduce a confounder because they slow the progression of diabetic retinopathy [11] were taken by only two of the G6PD-sufficient and one of the G6PD-deficient patients.

These considerations suggest that G6PD deficiency accelerates the microvascular complications of diabetes and that, of the many consequences of G6PD deficiency, those that can enhance the damage caused by diabetes outweigh those that could be protective. The decreased supply of NADPH, by limiting the regeneration of reduced glutathione, may magnify any level of oxidant stress occurring in diabetes. Additionally, if the observations made in cultured endothelial cells and in animals [3] apply to human G6PD deficiency, the decreased NADPH supply could affect eNOS activity, and thus reduce NO availability and endothelial performance. Of note, a facet of the impaired endothelial performance in in vitro and in vivo models of G6PD deficiency is an attenuated response to vascular endothelial growth factor and decreased angiogenesis [3]. Our diabetic patients with G6PD deficiency showed that the endothelial cells of retinal vessels respond to angiogenic cues and form new vessels. A recent report on pterygium (a benign growth of conjunctival fibroblasts) documents that nucleated cells of G6PD-deficient individuals, when in need of NADPH and pentoses for the synthetic activities of rapid growth, can compensate for the instability of G6PD by increasing its production [7].

Although this was a small study that awaits confirmation, it suggests that diabetic patients with G6PD deficiency can benefit from an additional level of intensive treatment to lessen the risk or severity of complications. It also encourages the testing of hypotheses to explain how G6PD deficiency augments the effects of diabetes on the microvasculature. One testable hypothesis is that G6PD-deficient patients have decreased NO availability and endothelial dysfunction. Experimental data in favour or against this hypothesis could inform therapeutic approaches to diabetic retinopathy.

Notes

Acknowledgements

We are grateful to M. Fossarello, Director of the Ophthalmology Clinic, Ospedale San Giovanni di Dio, University of Cagliari for making available for this study facilities and personnel; S. Pisano of the Ophthalmology Clinic, Ospedale San Giovanni di Dio for taking the eye photographs; and F. Mastinu and M. Mastinu for referring patients. The study was supported by a grant from the Juvenile Diabetes Research Foundation, a grant-in-aid from the Massachusetts Lions Eye Research Fund, and a generous donation from B. Shaw to M. Lorenzi.

Duality of interest

The authors declare that there is no duality of interest associated with this manuscript.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • G. Cappai
    • 1
  • M. Songini
    • 2
  • A. Doria
    • 3
  • J. D. Cavallerano
    • 4
  • M. Lorenzi
    • 5
  1. 1.Ophthalmology ClinicOspedale San Giovanni di DioCagliariItaly
  2. 2.Diabetes CenterOspedale San MicheleCagliariItaly
  3. 3.Joslin Diabetes CenterHarvard Medical SchoolBostonUSA
  4. 4.Beetham Eye Institute at Joslin Diabetes CenterHarvard Medical SchoolBostonUSA
  5. 5.Schepens Eye Research InstituteHarvard Medical SchoolBostonUSA

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