Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
Upon stimulation of insulin signalling or contraction-induced AMP-activated protein kinase (AMPK) activation, the glucose transporter GLUT4 and the long-chain fatty acid (LCFA) transporter CD36 similarly translocate from intracellular compartments to the plasma membrane of cardiomyocytes to increase uptake of glucose and LCFA, respectively. This similarity in regulation of GLUT4 traffic and CD36 traffic suggests that the same families of trafficking proteins, including vesicle-associated membrane proteins (VAMPs), are involved in both processes. While several VAMPs have been implicated in GLUT4 traffic, nothing is known about the putative function of VAMPs in CD36 traffic. Therefore, we compared the involvement of the myocardially produced VAMP isoforms in insulin- or contraction-induced GLUT4 and CD36 translocation.
Five VAMP isoforms were silenced in HL-1 cardiomyocytes. The cells were treated with insulin or the contraction-like AMPK activator oligomycin or were electrically stimulated to contract. Subsequently, GLUT4 and CD36 translocation as well as substrate uptake were measured.
Three VAMPs were demonstrated to be necessary for both GLUT4 and CD36 translocation, either specifically in insulin-treated cells (VAMP2, VAMP5) or in oligomycin/contraction-treated cells (VAMP3). In addition, there are VAMPs specifically involved in either GLUT4 traffic (VAMP7 mediates basal GLUT4 retention) or CD36 traffic (VAMP4 mediates insulin- and oligomycin/contraction-induced CD36 translocation).
The involvement of distinct VAMP isoforms in both GLUT4 and CD36 translocation indicates that CD36 translocation, just like GLUT4 translocation, is a vesicle-mediated process dependent on soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. The ability of other VAMPs to discriminate between GLUT4 and CD36 translocation allows the notion that myocardial substrate preference can be modulated by these VAMPs.
KeywordsCardiomyocytes CD36 Contraction GLUT4 Insulin SNARE VAMP
(phospho-)AMP-activated protein kinase
Mammalian expression vector containing the human cytomegalovirus promoter
Long-chain fatty acid
Protein kinase B
SNARE with glutamine in SNARE motif
SNARE with arginine in SNARE motif
Small interfering RNA
Soluble NSF attachment protein
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor
Vesicle-associated membrane protein
Glucose and long-chain fatty acids (LCFA) are the predominant substrates for energy provision of the contracting heart. The major cardiac substrate transporters in the heart are GLUT4 (glucose) and CD36 (LCFA), both of which cycle between intracellular stores and the plasma membrane (for review see Schwenk et al. ). Translocation of GLUT4 and CD36 to the plasma membrane is induced under conditions of elevated insulin levels (e.g. postprandially) and under conditions of increased ATP demand (e.g. upon increased contraction), involving activation of insulin signalling and AMP-activated protein kinase (AMPK), respectively.
In the early insulin-resistant heart there is a permanent relocation of CD36 from endosomes to the plasma membrane while GLUT4 distribution appears not yet affected . The constantly elevated presence of CD36 at the plasma membrane fuels a vicious cycle of increased CD36-mediated LCFA uptake and subsequent accumulation of toxic lipid metabolites (lipotoxicity) [2, 3]. Over time, lipotoxicity will lead to aggravation of insulin resistance, and finally to cardiac dysfunction . In order to explain the selective increase in sarcolemmal content of CD36 in the early insulin-resistant heart, there must be alterations in the trafficking machinery of CD36. Given that sarcolemmal CD36 relocation is an early key event in diabetes-related cardiac dysfunction, it is of pivotal importance to identify the trafficking machinery involved in the subcellular distribution of CD36, especially in relation to that of GLUT4.
The subcellular distribution of GLUT4 and CD36 is regulated by soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptors (SNAREs), the mechanistical core complexes of membrane fusion . Historically, SNAREs have been classified into vesicle-associated SNAREs (v-SNAREs) and target-membrane-associated SNAREs (t-SNAREs). However, more recent classifications reflect the conserved amino-acid residues within the SNARE motifs, which are either a glutamine (Q-SNAREs) or an arginine (R-SNAREs). When the vesicle-associated R-SNAREs interact with a specific subset of Q-SNAREs at the target membrane, a hetero-oligomeric SNARE complex is formed that catalyses the fusion of the vesicle with the target membrane.
While there are several subfamilies of t-SNAREs (syntaxins, SNAPs, Sec proteins), vesicle-associated membrane proteins (VAMPs) represent all members of the v-SNARE subfamily . There are seven identified VAMP isoforms: VAMP1/synaptobrevin1, which is involved in synaptic vesicle fusion  and appears to be absent in muscle tissues ; VAMP2/synaptobrevin2; VAMP3/cellubrevin; VAMP4; VAMP5/myobrevin, which is predominantly produced in heart and skeletal muscle ; VAMP7/TI-VAMP; and VAMP8/endobrevin . Insulin-stimulated translocation of GLUT4 involves VAMP2, which interacts with the t-SNAREs syntaxin4 and SNAP23 at the plasma membrane [9, 10]. Furthermore, VAMP7 has been shown to mediate GLUT4 translocation on hyperosmotic shock . These findings suggest that different VAMP isoforms might be involved in the translocation of GLUT4 upon different stimuli. However, nothing is known about the role of VAMPs in CD36 trafficking.
The purpose of this study was to investigate the involvement of each of the myocardial VAMPs in insulin- and contraction-stimulated GLUT4 and CD36 translocation to the plasma membrane. HL-1 atrial cardiomyocytes were used in which the genes encoding the different VAMP isoforms were transiently knocked down using small interfering RNAs (siRNAs). In these cells, plasmalemmal GLUT4 and CD36, as well as glucose and palmitate uptake, were measured after activation of insulin or contraction signalling. The data presented in this study disclose the involvement of distinct VAMPs in the cellular distribution of GLUT4 and CD36 and their importance for the insulin- and contraction-stimulated translocation of both transporters to the plasma membrane of cardiomyocytes.
Antibodies, siRNAs and plasmids
Antibodies against VAMP2 and VAMP3 were obtained from Abcam (Cambridge, UK), against VAMP4 from Novus Biologicals (Littleton, CO, USA), against VAMP5 from Synaptic Systems (Göttingen, Germany) and against VAMP7 and GLUT4 from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against the myc epitope, protein kinase B (PKB/AKT), phospho-PKB/AKT(Ser473), phospho-acetyl-coA carboxylase (pACC; Ser79), phospho-AMPKα (pAMPK; Thr172) and cleaved caspase-3 were from Cell Signaling (Danvers, MA, USA), and against mouse CD36 from Chemicon (Billerica, MA, USA). siRNAs against VAMP2/synaptobrevin2 (CCGATCTTTCCCTTATCTTtt), VAMP3/cellubrevin (GCTCATGCTCTTATGTTAGtt), VAMP4 (CGTACGTTTGAGCTTATAAtt), VAMP5/myobrevin (GGGAAGGCTGAATGACTGCtt) and VAMP7/TI-VAMP (GGAAATTCATGTGACTATGtt) were from Ambion (Applied Biosystems, Austin, TX, USA). The plasmid coding for GLUT4myc was kindly provided by J. Eckel (German Diabetes Center, Düsseldorf, Germany).
Cell culture of HL-1 atrial cardiomyocytes
HL-1 cells were kindly provided by W. Claycomb (Louisiana State University, New Orleans, LA, USA), cultured in Claycomb medium (supplemented with 10% FCS, 0.1 mmol/l noradrenaline [norepinephrine], 2 mmol/l l-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin) at 37°C and 5% CO2. Cells were split twice a week after reaching confluence.
HL-1 cells were electrically paced using the C-Pace Stimulator and six-well C-Dish Culture Dish Electrodes from IonOptix (Milton, MA, USA). Prior to electric-field stimulation, cells were incubated in depletion medium (DMEM low glucose, supplemented with 2 mmol/l l-glutamine, 100 μmol/l non-essential amino acids (NEAA), 100 U/ml penicillin and 100 μg/ml streptomycin) for 16 h at 37°C and 5% CO2. Electric-field stimulation was performed for 30 min at 40 V with a frequency of 2 Hz and a pulse duration of 15 ms.
Content of adenosine phosphates
Pelleted HL-1 cells were freeze dried then extracted with 1.3 mol/l perchloric acid and neutralised with 1 mol/l KHCO3. Thereafter, concentrations of ATP, ADP and AMP were determined by high-performance liquid chromatography as described previously by Luiken et al. .
Transfection of HL-1 cells
Plates (48 well) were seeded with 80,000 cells per well 24 h before transfection with double-stranded siRNAs. Transfection of siRNA was done with 25 pmol siRNA and 1 μl Lipofectamine-2000 (Invitrogen, San Diego, CA, USA) per well in antibiotic- and noradrenaline-free culture medium. Cotransfection of siRNA with pCMV-GLUT4myc was performed with 20 pmol siRNA, 0.15 μg plasmid DNA and 1 μl Lipofectamine-2000 per well in antibiotic- and noradrenaline-free culture medium. After 6 h, the medium was changed to regular culture medium. Cell lysis or functional assays were performed 48 h after siRNA transfection.
CD36 cell surface staining
Plasmalemmal CD36 was measured 48 h after transfection of siRNA into HL-1 cardiomyocytes. Prior to measuring CD36, cells were incubated in depletion medium for 16 h at 37°C and 5% CO2. Translocation of CD36 was induced by incubation with 200 nmol/l insulin or 1 μmol/l oligomycin, or by electric-field stimulation with 2 Hz pacing for 30 min at 37°C and 5% CO2. Afterwards, cells were fixed with 4% (wt/vol.) formaldehyde twice for 10 min at room temperature and blocked for 1 h with PBS containing 1% (wt/vol.) non-fat dried milk and 0.05% (vol./vol.) Tween 20. Cells were incubated with an anti-CD36 antibody (1:2,000 in blocking buffer) for 30 min at room temperature and subsequently washed three times with blocking buffer. Next, cells were incubated with a horseradish peroxidase (HRP)-linked secondary antibody (1:4,000 in blocking buffer) for 30 min at room temperature. After extensive washing with blocking buffer and PBS, an ortho-phenylenediamine–H2O2 solution was added as a substrate for the bound HRP. The reaction was carried out at room temperature and stopped after 30 min by addition of 1 mol/l H2SO4. Colour development, representative of the amount of CD36 present at the plasma membrane, was quantified by measurement of the absorbance at 490 nm. The background signal of the control (incubation without primary antibody) was subtracted from the raw data.
GLUT4myc cell surface staining
In contrast to CD36, GLUT4 is a transmembrane protein without a large extracellular epitope. Therefore, it cannot be detected by an antibody when integrated with the plasma membrane. This problem can be solved by overproduction of a GLUT4 variant carrying an myc tag on its first extracellular epitope . When properly integrated with the plasma membrane the myc epitope of GLUT4myc is extracellular and can easily be detected. In our study, plasmalemmal GLUT4myc was measured 48 h after cotransfection of the plasmid coding for GLUT4myc and the siRNAs. The staining of plasmalemmal GLUT4myc is done with a primary antibody against myc and an appropriate HRP-linked secondary antibody. Aside from these details, the GLUT4myc surface staining is homologous to the method of CD36 cell-surface staining. The background signal of the controls (1, incubation without primary antibody; 2, untransfected cells) was subtracted from the raw data.
Glucose and palmitate uptake
Substrate uptake into HL-1 cardiomyocytes was measured 48 h after transfection of the HL-1 cardiomyocytes seeded on pre-coated glass slides. Serum-depleted cells were treated with 200 nmol/l insulin, 1 μmol/l oligomycin or electrical pacing (2 Hz) for 30 min at 37°C. Subsequently, palmitate (coupled to BSA in a palmitate/BSA ratio of 1:3) and deoxy-d-glucose were added to final concentrations of 20 and 4 μmol/l, respectively, with tracer amounts of [14C]palmitate and 2-deoxy-d-[3H]glucose. After 10 min, uptake was terminated and unbound substrate removed by washing the cells with ice-cold depletion medium containing 0.2 mmol/l phloretin. After transfer of the glass slides into new culture dishes, cells were lysed by addition of 1 mol/l NaOH. Subsequently, incorporated glucose and palmitate were measured by scintillation counting of 14C and 3H.
Cell lysis and western blotting
Cell lysis and western blotting were performed as described earlier by van Oort et al. .
All data are presented as means ± SEM. Statistical analysis was performed by using Student’s t test or ANOVA (including Newman–Keuls multiple comparison test) and statistical analysis software Prism 4 (GraphPad Software). A p value of <0.05 was considered statistically significant.
Characterisation of HL-1 cells
HPLC analysis of adenosine phosphates in HL-1 cells
ATP (μmol/g dry weight)
1.57 ± 0.05
1.55 ± 0.13
1.15 ± 0.22*
0.69 ± 0.03*
ADP (μmol/g dry weight)
1.77 ± 0.28
1.25 ± 0.20
1.70 ± 0.19
1.25 ± 0.05
AMP (μmol/g dry weight)
0.27 ± 0.09
0.21 ± 0.01
0.41 ± 0.10
0.31 ± 0.05
0.17 ± 0.06
0.14 ± 0.02
0.36 ± 0.18*
0.45 ± 0.09*
Abundance of GLUT4myc and CD36 at the plasma membrane after VAMP knockdown
Effects of VAMP3 knockdown on electric-field stimulated GLUT4myc and CD36 translocation
GLUT4 and CD36 dynamically traffic between subcellular compartments and the plasma membrane in cardiomyocytes. Insulin and contraction trigger the accumulation of both substrate transporters at the plasma membrane to increase the uptake of glucose and LCFA. In the present study, we investigated the involvement of VAMPs in the process of GLUT4 and CD36 translocation to the plasma membrane on stimulation with insulin and increased AMPK activity. We identified myocardial VAMP isoforms that: (1) are shared by the GLUT4 and CD36 trafficking machineries but discriminate between insulin-stimulated and contraction-induced translocation processes; (2) discriminate between CD36 and GLUT4 traffic; and (3) contribute differently to the subcellular distribution of these substrate transporters. Interestingly, it is of note that despite residual levels of VAMP of 20–50%, the inhibitory effects of these gene knockdowns on stimulated transporter translocation were complete. More specifically, we observed no linear correlation between knockdown efficiency of each of the VAMPs and changes in plasmalemmal transporter abundance when comparing the results from the individual knockdown experiments. This indicates that GLUT4 and CD36 trafficking in cardiomyocytes is very sensitive to already small modulations of VAMP abundance.
Selective VAMPs are necessary for both GLUT4 and CD36 translocation, but discriminate between insulin- and contraction-induced translocation
Previous studies showed remarkable similarity between the regulation of GLUT4 and CD36 translocation, especially in the sense that both depend on the same signalling processes (i.e. the phosphoinositide-3 kinase [PI3K]/AKT axis upon insulin stimulation and the LKB1/AMPK axis upon contraction stimulation ). The present study further reveals that selected VAMPs (i.e. VAMP2, VAMP3 and VAMP5) are involved in the trafficking of both GLUT4 and CD36. Insulin-stimulated translocation of GLUT4 and CD36 was dependent on VAMP2 and VAMP5. The role of VAMP2 in insulin-stimulated GLUT4 translocation has been well established in skeletal muscle cells and adipocytes [9, 21]. However, the additional involvement of VAMP5 discloses a difference between cardiomyocytes and adipocytes . Still, the function of VAMP5 is rather complex as it is additionally involved in contraction-induced GLUT4 translocation. The mechanism by which insulin signals to VAMP2 and VAMP5 could include PI3K-mediated activation of protein kinase C ζ . The notion that both VAMP2 and VAMP5 are necessary for insulin-stimulated transporter translocation indicates that these VAMPs fulfil non-complementary roles in this process. Perhaps these VAMPs operate at different steps in the translocation process.
In contrast to the findings with VAMP2, we found VAMP3 to be selectively involved in contraction-induced translocation of both GLUT4 and CD36. Previous evidence for a role of VAMP3 in substrate-transporter translocation comes from studies in which VAMP3, just like VAMP2, has been shown to co-localise with GLUT4 [23, 24], whereas this has not yet been determined for CD36. However, functional evidence of the involvement of VAMP3 in contraction-induced transporter translocation is lacking in these previous studies. Our observation that VAMP3 is involved in contraction-induced transporter translocation is, however, difficult to reconcile with a study in rat skeletal muscle in which VAMP2, VAMP5 and VAMP7, but not VAMP3, migrated to the plasma membrane together with GLUT4 upon contraction stimulation . The observation by Rose et al. that the plasmalemmal presence of VAMP3 is not increased upon contraction might be explained by a rapid dissociation of the VAMP3-containing SNARE complex by NSF and SNAP at the plasma membrane [25, 26]. However, tissue differences might also explain these controversial observations.
Taken together, the shared involvement of distinct VAMPs in both GLUT4 and CD36 translocation indicates that CD36 translocation, just like GLUT4 translocation, is a vesicle-mediated process dependent on SNARE-complex formation. Moreover, this shared involvement further emphasises the similarity in the protein machinery regulating both processes.
Selective VAMPs can discriminate between GLUT4 and CD36 translocation
Besides the shared involvement of specific VAMPs in both GLUT4 and CD36 translocation, there are also VAMPs that discriminate between GLUT4 and CD36 translocation. We found VAMP4 to be involved specifically in CD36 translocation induced either by insulin or contraction. Hence, this VAMP isoform is an essential component of the CD36-dedicated trafficking machinery that translocates CD36 to the plasma membrane independently from GLUT4. VAMP5 is specifically involved in contraction-induced translocation of GLUT4, but not CD36. This contrasts with the involvement of VAMP5 in insulin-induced translocation of both transporters and shows that the involvement of VAMP5 in the regulation of transporter translocation is complex. Furthermore, the function of VAMP5 appears to be cell-type specific. While VAMP5 is not involved in GLUT4 trafficking in adipocytes , we and others could show its involvement in GLUT4 translocation in contracting myocytes .
Selective VAMPs contribute differently to the basal subcellular distribution of substrate transporters
Trafficking proteins are not only involved in stimulus-induced translocation processes, but also in the subcellular retention of a given recycling protein, so that upon termination of the stimulus, this protein returns into its intracellular storage compartment . Hence, VAMPs might not only be involved in the translocation of GLUT4 and CD36 to the plasma membrane but also in their intracellular sorting. A reduction in specific VAMPs could result in an increased basal presence of the substrate transporters at the plasma membrane, either through unregulated dispersion or inhibited internalisation.
The present findings indicate that VAMP5 and VAMP7 mediate basal GLUT4 retention. Interestingly, studies in C2C12 myoblasts have revealed the presence of VAMP5 at the plasma membrane . Combining this finding with the present data suggests that the plasmalemmal presence of VAMP5 is important in GLUT4 internalisation. As we also established that VAMP5 is additionally involved in insulin- and contraction-induced GLUT4 translocation, VAMP5 appears to have a dual function in the regulation of GLUT4 distribution. Furthermore, we observed a discrepancy between GLUT4 abundance at the plasma membrane and glucose uptake in VAMP5-depleted cells: the latter was disproportionally increased. This discrepancy is likely to be attributed to GLUT1, which is the second most important glucose transporter in the heart [28, 29]. Inhibition of GLUT4-mediated glucose uptake by indinavir revealed that the residual glucose uptake (via GLUT1) was increased by 1.5-fold in VAMP5-depleted cells. Therefore, VAMP5 might be involved in GLUT1 internalisation as well as GLUT4 internalisation under basal conditions. Alternatively, plasmalemmal GLUT1 might be upregulated in VAMP5-depeleted cells as a secondary effect to compensate for the loss of plasmalemmal GLUT4. This has already been observed in cardiomyocytes and adipocytes lacking GLUT4 [30, 31]. Furthermore, a compensatory action of GLUT1 might serve as an explanation for the incomplete inhibition of insulin-stimulated glucose uptake in VAMP2-depleted cells and of contraction-stimulated glucose uptake in VAMP3-depleted cells in comparison with complete inhibition of GLUT4 translocation in both cases.
GLUT4, but not CD36, also accumulated at the plasma membrane after VAMP7 knockdown, and even to a markedly greater extent than after VAMP5 knockdown. However, in contrast to VAMP5 knockdown, insulin and oligomycin further increased the sarcolemmal abundance of GLUT4 in VAMP7-depleted cells. Hence, VAMP7 solely functions in GLUT4 internalisation and, unlike VAMP5, has no additional role in stimulus-induced GLUT4 translocation.
We also unveiled that a VAMP isoform—VAMP2—is involved in basal CD36 retention. This ability of VAMP2 to reduce basal levels of CD36 at the plasma membrane is not accompanied by a similar effect of this VAMP isoform on basal GLUT4 retention. Therefore, next to VAMP5 and VAMP7, VAMP2 knockdown also specifically increases the sarcolemmal presence of one of the two transporters under basal conditions. Because we also found VAMP2 to be involved in insulin-stimulated CD36 translocation, this additional involvement in basal CD36 retention points to a dual role of VAMP2 in the regulation of CD36 trafficking. Hence, the dual role of VAMP2 in CD36 trafficking mirrors the dual role of VAMP5 in GLUT4 traffic, and further indicates that both processes are similarly regulated though, in part, by different VAMP isoforms.
These findings indicate the potential of VAMPs to serve as targets for the so-called metabolic modulation therapy [32, 33]. For instance, pharmacological strategies to stimulate cardiac glucose uptake via increasing myocardial VAMP2 production or decreasing myocardial VAMP4/VAMP7 production is expected to be beneficial for the diabetic heart in which glucose uptake is repressed in favour of LCFA. Interestingly, VAMP2-silenced HL-1 cells resemble insulin-resistant cardiomyocytes because: (1) use of LCFA is increased already in the basal state; (2) insulin has lost the ability to stimulate LCFA and glucose uptake; but (3) contraction-induced glucose and LCFA uptake are unimpaired. Similar changes occur in the cardiac and skeletal muscle of insulin-resistant obese Zucker rats [2, 34]. Furthermore, VAMP2 production is decreased in the retinas from streptozotocin-diabetic rats  and in insulin-secreting cells after 72 h exposure to palmitate . Whether inhibition of VAMP2 also takes place in the diabetic heart remains to be determined.
This study was supported by the European Community (Integrated Project LSHM-CT-2004-005272, Exgenesis), the transnational University Limburg, the Natural Sciences and Engineering Research Council of Canada, and the Heart and Stroke Foundation of Ontario. A. Bonen is Canada Research Chair in Metabolism and Health.
Duality of interest
The authors report that there is no duality of interest associated with this manuscript.
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