The glucose-6-phosphatase catalytic subunit (G6PC) plays a key role in hepatic glucose production by catalysing the final step in gluconeogenesis and glycogenolysis. Peroxisome proliferator activated receptor gamma coactivator-1α (PGC-1α) stimulates mouse G6pc–luciferase fusion gene expression through hepatocyte nuclear factor-4α (HNF-4α), which binds an element located between −76 and −64 in the promoter. The aim of this study was to compare the regulation of mouse G6pc and human G6PC gene expression by PGC-1α.
PGC-1α action was analysed by transient transfection and gel retardation assays.
In H4IIE cells, PGC-1α alone failed to stimulate human G6PC–luciferase fusion gene expression even though the sequence of the −76 to −64 HNF-4α binding site is perfectly conserved in the human promoter. This difference could be explained, in part, by a 3 bp sequence variation between the mouse and human promoters. Introducing the human sequence into the mouse G6pc promoter reduced PGC-1α-stimulated fusion gene expression, whereas the inverse experiment, in which the mouse sequence was introduced into the human G6PC promoter, resulted in the generation of a G6PC–luciferase fusion gene that was now induced by PGC-1α. This critical 3 bp region is located immediately adjacent to a consensus nuclear hormone receptor half-site that is perfectly conserved between the mouse G6pc and human G6PC promoters. Gel retardation experiments revealed that this 3 bp region influences the affinity of HNF-4α binding to the half-site.
These observations suggest that PGC-1α may be more important in the control of mouse G6pc than human G6PC gene expression.
Glucose-6-phosphatase catalyses the terminal step of gluconeogenesis and glycogenolysis, the hydrolysis of inorganic phosphate from glucose-6-phosphate . Glucose-6-phosphatase is a multi-component enzyme system consisting of transporters for glucose-6-phosphate, glucose and inorganic phosphate, and a catalytic subunit whose active site is orientated towards the lumen of the endoplasmic reticulum . In mammals, glucose-6-phosphatase activity is highest in the liver and kidney, but activity is also detected at lower levels in the brain, small intestine and pancreatic islets . Hepatic glucose-6-phosphatase activity is critical for maintaining plasma glucose levels between meals. Mutations in the gene encoding the glucose-6-phosphatase catalytic subunit (G6PC) result in glycogen storage disease type 1a (von Gierke’s disease), which is characterised by life-threatening hypoglycaemia, growth retardation, renal dysfunction and hepatomegaly . In contrast, increased G6PC mRNA and G6PC activity are thought to contribute to the elevated hepatic glucose production (HGP) characteristic of type 1 and type 2 diabetes [3, 4].
Peroxisome proliferator activated receptor (PPAR)-gamma-coactivator 1α (PGC-1α) is an important regulator of metabolism, contributing to transcriptional activation of genes involved in adaptive thermogenesis, mitochondrial biogenesis, respiration and gluconeogenesis [5, 6]. In the liver PGC-1α is upregulated during fasting and in diabetes and it stimulates expression of the G6PC and PEPCK (also known as PCK1) genes . Two groups of investigators have generated mice with a global deletion of the Pgc-1α gene. Both reported that the mice were viable, but had multi-system abnormalities that included diminished mitochondrial respiratory capacity, vacuolar lesions in the central nervous system and cold intolerance [8, 9]. However, these groups reported variable effects of PGC-1α deletion on hepatic gluconeogenic gene expression and fasting blood glucose levels. Lin et al.  observed hypoglycaemia in the fasted state and reduced HGP. However, instead of the expected impairment, G6pc and Pepck gene expression were surprisingly elevated in the fed state to a level equivalent to that in the fasted state. This elevation of G6pc and Pepck expression was potentially caused by elevated hepatic C/ebpβ (also known as Cebpb) gene expression . In contrast, in their mice, Leone et al.  observed euglycaemia in the fasted state with no elevation of G6pc, Pepck or C/ebpβ expression in the fed state and the same induction following fasting as seen in wild-type mice. The reasons for these differences are unclear. Interestingly, although Leone et al.  observed no change in G6pc and Pepck expression and euglycaemia in the fasted state, HGP and gluconeogenic flux are altered in these mice as a consequence of altered tricarboxylic acid cycle flux . In mice with a liver-specific deletion of the Pgc-1α gene, G6pc and Pepck expression were normal in the fed state but their induction by fasting was impaired . The observation that the effect of fasting was not abolished is probably not explained by the presence of PGC-1β because, although induced by fasting , it only weakly activates G6pc and Pepck expression . However, the observation is consistent with the fact that ongoing protein synthesis is not required for the hormonal regulation of these genes . As such, PGC-1α is not essential for G6pc and Pepck expression, but does play an important role as a transcriptional amplifier .
At the molecular level, PGC-1α interacts with multiple transcription factors including PPARα, PPARγ, nuclear respiratory factor-1, the glucocorticoid receptor, oestrogen-related receptors, liver X receptor, pregnane X receptor, myocyte enhancer factor-2 and hepatocyte nuclear factor-4α (HNF-4α) [5, 6]. HNF-4α is a member of the steroid hormone receptor superfamily and is present in the liver, intestine, kidney, pancreatic islets, stomach and skin [16, 17]. Hnf-4α (also known as Hnf4a) knockout mice have an embryonic lethal phenotype , whereas liver-specific Hnf-4α-deficient mice exhibit defective lipid homeostasis and weight loss . Mutation of HNF4A results in maturity onset diabetes of the young type 1, which is also characterised by altered lipid homeostasis as well as defective glucose-stimulated insulin secretion . HNF-4α is essential for the induction of G6pc and Pepck expression by PGC-1α .
We have previously shown that in rat H4IIE hepatoma cells the activation of mouse G6pc expression by PGC-1α was mediated through an HNF-4α binding site located between −76 and −64 , whereas Rhee et al.  showed in immortalised mouse hepatocytes that the activation of human G6PC expression by PGC-1α was mainly mediated through two HNF-4α binding sites located between −296 and −284, and −256 and −244. Because these latter sites are poorly conserved in the mouse G6pc promoter, this observation appeared to explain the differences between the reported results. However, in the current study we demonstrate that the PGC-1α responsiveness of the mouse G6pc and human G6PC promoters are fundamentally different. Thus, when the mouse G6pc and human G6PC promoters were compared in the same H4IIE cell line only mouse G6pc–luciferase fusion gene expression was induced by PGC-1α. The molecular basis for this difference was mapped to a sequence variation between the mouse and human promoters that affects the affinity of HNF-4α binding. These results suggest that PGC-1α may be more important for the regulation of mouse G6pc than human G6PC expression, a difference that could contribute to the observed higher rate of fasting HGP in mice than humans.
The generation of a mouse G6pc−luciferase fusion gene, containing promoter sequence located between −484 and +66, relative to the transcription start site, in the pGL3 MOD vector has been previously described . PCR reactions with this plasmid as the template were used to generate mouse G6pc−luciferase fusion genes containing wild-type or mutated promoter sequence between −85 and +1. A human G6PC−luciferase fusion gene, containing promoter sequence located between −826 and +63, was generated by PCR using human genomic DNA as the template. PCR reactions with this plasmid as the template were used to generate human G6PC−luciferase fusion genes containing wild-type or mutated promoter sequence between −83 and +7. All promoter fragments generated by PCR were completely sequenced to verify the absence of polymerase errors.
Expression vectors encoding the rat glucocorticoid receptor (pRSV-GR) , rat HNF-4α (pSG5-HNF4α) ) and human forkhead box O1 (FOXO1; pcDNA3-FKHR)  were generously provided by K. Yamamoto (University of California, San Francisco, CA, USA), D. Granner (Vanderbilt University School of Medicine, Nashville, TN, USA) and D. Powell (Baylor College of Medicine, Houston, TX, USA), respectively.
A pBJ5 expression vector encoding a putative mouse PGC-1α splice variant representing the entire 797 amino acid PGC-1α open reading frame  minus amino acids 476 to 487 has been previously described . A full-length pBJ5-PGC-1α expression vector was constructed by inserting the missing PGC-1α sequence, which was isolated from I.M.A.G.E. clone # 4193174 (Open Biosystems, Huntsville, AL, USA).
Cell culture and transient transfections
Rat H4IIE hepatoma cells were cultured and transiently transfected in suspension using the calcium phosphate–DNA co-precipitation method as previously described .
Luciferase assays were performed using the Dual-Luciferase Reporter Assay System (Promega Corp., Madison, WI, USA) according to the manufacturer’s instructions. In these experiments we saw no effect of PGC-1α or HNF-4α overexpression on Renilla luciferase activity. Therefore, for comparisons of PGC-1α- and HNF-4α-regulated gene expression, firefly luciferase activity directed by the various fusion gene constructs was expressed relative to Renilla luciferase activity in the same sample. Each construct was analysed in duplicate in multiple transfections, as specified in the figure legends, using multiple independent plasmid preparations.
Nuclear extract preparation and gel retardation assays
H4IIE nuclear extract was prepared as previously described . Oligonucleotides representing sense and antisense wild-type and mutated G6pc promoter sequences as described in the text were synthesised with BamHI compatible ends by Operon Technologies (Alameda, CA, USA) and subsequently annealed and labelled with [α32P]dATP using the Klenow fragment of Escherichia coli DNA Polymerase I to a specific activity of approximately 9 × 104 Bq/pmol . Gel retardation assays were then performed exactly as previously described .
The transfection data were analysed for differences from the control values, as specified in the figure legends. Statistical comparisons were calculated using an unpaired Student’s t test. The level of significance was as indicated (two-sided test).
PGC-1α can selectively stimulate mouse G6pc−luciferase fusion gene expression
The rate of fasting HGP is tenfold higher in mice than humans [30, 31]. We have been interested as to whether differences in the regulation of G6PC gene transcription between mice and humans could partly explain this observation. A carbohydrate response element identified in the mouse and rat G6pc promoters is not conserved in the human G6PC promoter . However, the mechanism by which insulin suppresses both mouse G6pc– and human G6PC–luciferase fusion gene expression in human HepG2 hepatoma cells appears identical . Likewise, the synthetic glucocorticoid, dexamethasone, equally stimulates mouse G6pc– and human G6PC–luciferase fusion gene expression in rat H4IIE hepatoma cells (Fig. 1). Since PGC-1α markedly induces G6PC expression and HGP , we next compared the ability of PGC-1α to stimulate mouse G6pc– and human G6PC–luciferase fusion gene expression in rat H4IIE hepatoma cells. As previously reported , co-transfection of H4IIE cells with an expression vector encoding PGC-1α robustly induces the expression of a mouse G6pc−luciferase fusion gene containing promoter sequence between −484 and +66 (Fig. 2a). In contrast, expression of a human G6PC−luciferase fusion gene containing promoter sequence between −826 and +63 was unaffected (Fig. 2a). Although mouse PGC-1α was used in this experiment, mouse and human PGC-1α share 95% amino acid sequence homology (758/797), suggesting that limited differences in structural characteristics and binding properties exist between the two species. Indeed, the observation that the human promoter was unresponsive to PGC-1α at all concentrations tested (Fig. 2b) suggested that there is a fundamental difference between the mouse G6pc and human G6PC promoters rather than just a difference in sensitivity towards the action of PGC-1α.
Identification of sequence variations that contribute to the selective effect of PGC-1α on mouse G6pc−luciferase fusion gene expression
The results suggested that either the human promoter contained a species-specific element that repressed the PGC-1α response or that the mouse promoter contained a species-specific PGC-1α responsive element that is absent in the human promoter. Before addressing these possibilities, we first sought to define shorter regions of the mouse G6pc and human G6PC promoters that demonstrated a selective effect of PGC-1α.
The effect of PGC-1α on mouse G6pc−luciferase fusion gene expression was previously mapped to the promoter region between −85 and +66 and was shown to require an HNF-4α binding site located between −76 and −64 . The 5′ untranslated regions (5′ UTRs) of the mouse and human genes contain numerous sequence variations, whereas the mouse promoter sequence between −85 and +1 and the equivalent human sequence between −83 and +1 are highly conserved (Fig. 3). Deletion of the mouse 5′ UTR between +2 and +66 reduced the magnitude of PGC-1α-induced mouse G6pc−luciferase fusion gene expression indicating that element(s) in the 5′ UTR contribute to the PGC-1α response (data not shown). However, the −85 to +1 promoter region still mediated a PGC-1α response (Fig. 4a). In contrast, a similar region of the human G6PC promoter, located between −83 and +7, was unresponsive to PGC-1α (Fig. 4b). The 5′ UTR sequence between +1 and +7 is identical between mouse and human so this cannot explain the observed difference. This result implies that at least part of the differential regulation of mouse G6pc– and human G6PC–luciferase fusion genes by PGC-1α must be explained by sequence differences within the proximal promoter regions shown in Fig. 3.
Inspection of the mouse promoter sequence between −85 and +1 with the equivalent human sequence between −83 and +1 revealed only seven nucleotide differences, specifically one deletion and six substitutions (Fig. 3). The previously identified mouse HNF-4α binding site  is perfectly conserved in the human promoter (Fig. 3). To identify the sequence required for the selective effect of PGC-1α on mouse G6pc–luciferase fusion gene expression site, directed mutations were generated in the context of the mouse −85 to +1 G6pc promoter, designated hMUT 1, 2 and 4, in which the mouse sequence was substituted for that of the human (Fig. 3). G6pc−luciferase fusion genes containing these mutations were then transiently transfected into H4IIE cells and the effect of PGC-1α co-transfection was assessed. Figure 4a shows that switching the sequence of the MUT 2 and MUT 4 regions to that of the human had no effect on the PGC-1α response, whereas switching the sequence of the MUT 1 region to that of the human reduced the PGC-1α response. Significantly, Fig. 4b shows that when the inverse experiment was performed, in which the sequence of the MUT 1 region in the human promoter was switched to that of the mouse, expression of the resulting G6PC−luciferase fusion gene, designated −83/+7 hG6P mMUT 1, was induced by PGC-1α. This result suggests that the selective effect of PGC-1α on mouse G6pc−luciferase fusion gene expression can be explained, at least in part, by the differential binding of a factor to the MUT 1 region.
A conserved nuclear receptor half-site is required for the effect of PGC-1α on mouse G6pc–luciferase fusion gene expression
Analysis of the G6pc promoter sequence encompassing the MUT 1 region using MatInspector software  revealed the existence of a consensus nuclear receptor half-site adjacent to the MUT 1 region (Fig. 3). This element is perfectly conserved in the mouse G6pc and human G6PC promoters (Fig. 3). A mouse G6pc−luciferase fusion gene containing a site-directed mutation of this element, designated −85/+1 mG6p MUT 3, was transiently transfected into H4IIE cells and the effect of PGC-1α co-transfection was assessed. Figure 5 shows that mutating the MUT 3 region markedly reduced the PGC-1α response. These data demonstrated that, in addition to the HNF-4α binding site located between −76 and −64 , this nuclear receptor half-site was critical for the PGC-1α response.
Altered HNF-4α binding to a conserved nuclear receptor half-site contributes to the selective effect of PGC-1α on mouse G6pc−luciferase fusion gene expression
We reasoned that the scenario most likely to explain the data was one in which PGC-1α interacted with a member of the nuclear receptor superfamily bound to the MUT 3 region with the flanking sequence, as represented by the MUT 1 region, affecting binding affinity. Studies on PPARα binding to nuclear receptor half-sites have established a precedent for such a model . Gel retardation assays using the mouse G6pc promoter sequence between −62 and −29, which includes both the MUT 3 and MUT 1 regions (Fig. 3), as the labelled probe failed to detect specific protein−DNA binding (data not shown). However, this presumably reflects the low affinity of interaction of nuclear receptors with half-sites.
Since chromatin immunoprecipitation experiments have shown that HNF-4α binds this region of the G6pc promoter in intact H4IIE cells  the simplest model to explain the data is one in which HNF-4α binds the −76/−64 element as well as the MUT 3 region, with the MUT 1 flanking region affecting the affinity of HNF-4α binding (Fig. 3). To explore this possibility an oligonucleotide representing the mouse G6pc promoter sequence between −82 and −37, which contains the high-affinity −76/−64 HNF-4α binding site  and the putative HNF-4α binding half-site (Fig. 6a), was used as the labelled probe in gel retardation assays. The aim was to assess the ability of oligonucleotides representing the mouse G6pc promoter sequence between −82 and −55, which includes just the high-affinity −76/−64 HNF-4α binding site, or the mouse G6pc promoter sequence between −62 and −29, which includes just the MUT 3 and MUT 1 regions (Fig. 6a), to compete for HNF-4α binding. When the labelled double-stranded wild-type G6PC −82 and −37 oligonucleotide was incubated with nuclear extract prepared from H4IIE cells, a single major protein–DNA complex was detected (Fig. 6b). Supershift experiments using specific antisera have demonstrated that this complex contains HNF-4α . Competition experiments, in which a 100-fold molar excess of unlabelled DNA was included with the labelled probe, were used to correlate protein binding with the PGC-1α response. The wild-type G6PC −82/−37 oligonucleotide competed effectively for formation of the protein–DNA complex, as did the −82/−55 oligonucleotide that includes just the high-affinity −76/−64 HNF-4α binding site (Fig. 6b). Significantly, the wild-type G6PC −62/−29 oligonucleotide also competed for formation of the protein–DNA complex confirming that this promoter region can bind HNF-4α (Fig. 6b). Oligonucleotides, designated mG6P hMUT 1 and MUT 3 (Fig. 6a) that contain mutations identical to those described in the hMUT 1 (Fig. 4a) and MUT 3 (Fig. 5) fusion genes, competed poorly with the labelled probe for protein binding and less effectively than the WT G6P −62/−29 oligonucleotide (Fig. 6b). Figure 6c shows the results from multiple experiments using a variable molar excess of the various competitors. The observation that the MUT 3 oligonucleotide still competed slightly at high molar excess may be explained by the presence of a partial nuclear receptor half-site, AGGgCA, located between −35 and −30 (Fig. 6a). These results show that the binding of HNF-4α to the MUT 1 and MUT 3 regions correlates with the PGC-1α response.
In an attempt to further support this conclusion, protein binding to labelled probes representing the mouse −82/−37 region and equivalent human −81/−36 region was compared. No difference was detected (data not shown), suggesting that the use of labelled probes only detects HNF-4α binding to the conserved high-affinity HNF-4α binding site, and not the HNF-4α half-site. This is consistent with the observation that protein binding to the labelled −62/−29 probe was not detectable (see above). This result indicates that, while HNF-4α binding to the half-site can be implied from competition experiments (Fig. 6), it cannot be directly demonstrated using labelled probes in the gel retardation assay. Presumably this reflects the low affinity of HNF-4α binding to the half-site, such that HNF-4α dissociates from this site during electrophoretic separation of the bound and free probe. Similar situations have been reported in studies on the glucocorticoid receptor  and FOXO1 , where binding to low-affinity sites could only be implied through competition experiments and not directly demonstrated using labelled probes.
Overexpression of HNF-4α or FOXO1 enhances the action of PGC-1α on both mouse G6pc– and human G6PC–luciferase fusion gene expression
Since the data support a model in which the MUT 1 region affects the affinity of HNF-4α binding to the MUT 3 region, we reasoned that overexpression of HNF-4α might, through a mass action effect, result in binding of HNF-4α to the MUT 3 region in the human G6PC promoter despite the sub-optimal MUT 1 flanking sequence. Figure 7 shows that this is indeed the case. Although PGC-1α and HNF-4α had little effect alone, co-transfection of both PGC-1α and HNF-4α together resulted in a synergistic induction of human G6PC–luciferase fusion gene expression (Fig. 7). The effects of both HNF-4α and PGC-1α alone or in combination were enhanced when the sequence of the MUT 1 region in the human promoter was switched to that of the mouse (Fig. 7). Similarly, co-transfection of PGC-1α and HNF-4α also resulted in a synergistic induction of mouse G6pc–luciferase fusion gene expression, but in this case the effects of HNF-4α and PGC-1α, either alone or in combination, were reduced when the sequence of the MUT 1 region in the mouse promoter was switched to that of the human (Fig. 7). This again suggests that the sequence of the MUT 1 region affects both the affinity of HNF-4α binding and the PGC-1α response. The observation that overexpression of HNF-4α affects the PGC-1α response implies that the results of G6PC–luciferase fusion gene analyses will be dependent on variations in endogenous HNF-4α levels in H4IIE cells. This may explain why in some experiments the presence of the human MUT 1 sequence is associated with a complete lack of a PGC-1α response (Fig. 4b) whereas in other experiments a partial response remains (Fig. 4a).
Figure 8 shows similar experiments, in which the action of PGC-1α on G6PC–luciferase fusion gene expression was assessed in the presence or absence of FOXO1. As previously reported, both mouse G6pc–  and human  G6PC–luciferase fusion gene expression was stimulated by overexpression of FOXO1 (Fig. 8). In addition, co-transfection of PGC-1α and FOXO1 together resulted in a synergistic induction of mouse G6pc– and human G6PC–luciferase fusion gene expression (Fig. 8).
Our results demonstrate that, in H4IIE cells, PGC-1α can selectively stimulate mouse G6pc– but not human G6PC–luciferase fusion gene expression (Fig. 2). This differential regulation can be explained by a sequence variation between the mouse and human promoters that results in reduced binding of HNF-4α to the human promoter (Figs 3, 4, 5 and 6). When HNF-4α (Fig. 7) or FOXO1 (Fig. 8) are overexpressed, PGC-1α interacts synergistically with these factors to induce human G6PC–luciferase fusion gene expression, although the induction achieved is still less pronounced than that seen with the mouse promoter. These results imply that PGC-1α may be more important for the regulation of G6PC expression in mice than in humans and that the difference in PGC-1α regulation reported here has the potential to contribute to the tenfold higher rate of HGP in mice [30, 31]. In addition, the results suggest that the regulation of G6PC expression by PGC-1α is likely to be more sensitive to changes in levels of HNF-4α and FOXO1 in humans than mice.
Spiegelman and colleagues [20, 40] previously noted that PGC-1α alone had little effect on human G6PC–luciferase fusion gene expression in immortalised mouse hepatocytes and the data presented here provide an explanation for that observation. They also reported that co-transfection of PGC-1α with either FOXO1  or HNF-4α  resulted in a synergistic induction of fusion gene expression. The same synergistic interaction between PGC-1α and HNF-4α (Fig. 7) and FOXO1 (Fig. 8) is seen in H4IIE cells. With respect to the action of FOXO1, Puigserver et al.  presented a model in which PGC-1α interacted directly with FOXO1. However, mutation  or deletion  of the three insulin response sequences in the G6PC promoter had no effect on the PGC-1α response. Moreover, we recently showed that PGC-1α stimulates G6pc–luciferase fusion gene expression through HNF-4α and that the binding of FOXO1 to the G6pc promoter is neither required nor sufficient for this induction . We hypothesise that the synergism seen between FOXO1 and PGC-1α occurs at the level of the pre-initiation transcription complex. We envisage that both FOXO1, bound to multiple sites in the G6PC promoter, and PGC-1α tethered to the promoter through HNF-4α synergise to increase pre-initiation complex formation. This concept is consistent with the observation that both proteins can interact with other non-DNA bound factors [5, 6, 42]. For example, FOXO1 can bind the CREB binding protein KIX domain, located within the N-terminal activation domain , whereas PGC-1α can bind the C-terminal activation domain on the related protein p300 . The synergistic action of FOXO1 and PGC-1α on G6PC–luciferase fusion gene transcription could then feasibly be mediated through combined binding to CREB binding protein/p300. More recently, Matsumoto and colleagues  demonstrated in primary hepatocytes lacking FOXO1 that the induction of G6PC expression by PGC-1α was markedly reduced. This is consistent with the synergistic interaction between FOXO1 and PGC-1α seen in both H4IIE cells  and immortalised mouse hepatocytes . However, while the effect of PGC-1α on G6pc expression was markedly reduced in primary hepatocytes lacking FOXO1, a ~20-fold induction remained . In contrast, the ability of PGC-1α to stimulate G6pc expression in hepatocytes derived from mice lacking HNF-4α is completely lost . The results of Matsumoto and colleagues  are therefore consistent with our model, in which PGC-1α stimulates G6PC–luciferase fusion gene expression through HNF-4α, with the binding of FOXO1 to the G6PC promoter being neither required nor sufficient for this induction, although it does act synergistically to enhance the response .
Published reports suggest that this model is also consistent with the mechanism of action of PGC-1α on PEPCK expression. Thus, the action of PGC-1α on PEPCK fusion gene expression is mediated through HNF-4α and glucocorticoid receptor binding sites, whereas the FOXO1 binding site is not required for the PGC-1α response [7, 15]. In addition, the ability of PGC-1α to stimulate Pepck expression in hepatocytes derived from mice lacking HNF-4α is also completely lost .
forkhead box O1
glucose-6-phosphatase catalytic subunit
hepatic glucose production
hepatocyte nuclear factor-4α
peroxisome proliferator activated receptor gamma coactivator-1α
peroxisome proliferator activated receptor
- 5′ UTR:
5′ untranslated region
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We thank J. N. Boustead for constructing the full-length PGC-1α expression vector and several human G6PC−luciferase fusion genes. We also thank K. Yamamoto, D. Granner and D. Powell for providing the glucocorticoid receptor, HNF-4α and FOXO1 expression vectors, respectively. Research in the laboratory of R. M. O’Brien was supported by NIH grant DK56374 and by NIH grant P60 DK20593, which supports the Vanderbilt Diabetes Center Core Laboratory. M. M. Schilling was supported by the Vanderbilt Molecular Endocrinology Training Program (5 T 32 DK07563).
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
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Schilling, M.M., Oeser, J.K., Chandy, J.K. et al. Sequence variation between the mouse and human glucose-6-phosphatase catalytic subunit gene promoters results in differential activation by peroxisome proliferator activated receptor gamma coactivator-1α. Diabetologia 51, 1505 (2008). https://doi.org/10.1007/s00125-008-1050-8