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Diabetologia

, Volume 48, Issue 10, pp 1957–1962 | Cite as

Involvement of sulfatide in beta cells and type 1 and type 2 diabetes

  • K. Buschard
  • M. Blomqvist
  • T. Osterbye
  • P. Fredman
Review

Abstract

Mammalian tissues express β-isoforms of glycosphingolipids and, among these, sulfatide (sulphated galactosylceramide) is present in the beta cells, and it is here that the short fatty acid chain (C16) isoform is predominately found. In vitro studies have shown that sulfatide preserves insulin crystals and facilitates insulin monomerisation under certain biochemical conditions. It also activates beta cell potassium channels and moderates insulin secretion. Anti-sulfatide antibodies are seen in type 1 diabetes, and immunological presentation of glycosphingolipids by the non-classical CD1 molecules has recently been reported. It is via this mechanism that α-galactosylceramide and sulfatide are able to influence the innate immune system and inhibit autoimmunity, possibly through regulatory natural killer T cells. Administration of sulfatide substantially reduces the incidence of diabetes in non-obese diabetic mice and prevents antigen-induced experimental autoimmune encephalomyelitis in wild-type mice. Sulfatide has specific anti-inflammatory properties, increasing the number of CD3+CD25+ regulatory T cells and reducing production of several cytokines, including TNF-α. Patients with type 2 diabetes have low serum concentrations of sulfatide, and some animal models of type 2 diabetes have low pancreatic expression of C16:0 sulfatide; administration of this increases insulin secretion and improves first-phase insulin response in Zucker fatty rats. Glycosphingolipids in general, and sulfatide in particular, appear relevant to both type 1 and type 2 diabetes.

Keywords

CD1 Cytokines Galactosylceramide GalCer Glycosphingolipids Insulin Sulphated lactosylceramide Sulfatide SulphLacCer Type 1 diabetes Type 2 diabetes 

Abbreviations

CGT

UDP-galactosyl:ceramide galactosyltransferase

CST

ceramide galactosyl sulfotransferase

GalCer

galactosylceramide

MIP-1α

macrophage inflammatory protein-1α

NKT cell

natural killer T cell

NOD

non-obese diabetic

SulphLacCer

sulphated lactosylceramide

Introduction

Sulfatide is a glycosphingolipid, originally identified in neural tissue [1] and later in the islets of Langerhans [2]. It is also found in the kidneys, retina and associated with lipoproteins in the blood [3]. Sulfatide is an amphiphilic molecule composed of ceramide, galactose and sulphate (Fig. 1); the sulphate group has a negative charge and acidic properties. The molecular weight (800–900 Da) of each glycosphingolipid varies according to the composition of the hydrophobic moiety; in humans, this is primarily related to the length of the fatty acid chain, which generally consists of 16–24 carbon atoms. The names of the different isoforms are designated according to the length and extent of saturation of the fatty acid chain, e.g. the saturated isoform with 16 carbon atoms is denoted as C16:0 sulfatide. In the brain, long-chain forms are most frequent, whereas the pancreas contains considerable amounts of the short-chain variety (C16:0); in the pancreas of some species, 50% of sulfatide is found in this form [4]. Variations in fatty acid chain length influence the physical chemical properties of the sulfatide molecule.
Fig. 1

Chemical structure of sulfatide. Sulfatide consists of a sulphated galactose group attached to ceramide. The ceramide moiety is composed of a sphingosine base (dihydroxy sphingosine, d18:1) and a fatty acid with a chain length of 16–24 carbon atoms. *Possible position of hydroxylation of the fatty acid chain

Sulfatide is a derivative of galactosylceramide (GalCer), a glycosphingolipid produced by the addition of galactose to ceramide by the enzyme UDP-galactose:ceramide galactosyltransferase (CGT) [5]. Ceramide galactosyl sulfotransferase (CST) synthesises sulfatide through the addition of a sulphate group to GalCer [6]. Both CGT and CST have been cloned and knock-out mice generated [7, 8]; both types of genetically modified mice have serious neurological disorders and are unable to breed. CST also catalyses the synthesis of seminolipid and sulphated lactosylceramide (SulphLacCer).

Sulfatide and beta cells

Using the monoclonal antibody Sulph I [9], secretory granules containing insulin crystals were found to be stained in beta cells examined by electron microscopy [10] (Fig. 2 and Text box: Physiological functions of sulfatide in beta cells).

Physiological functions of sulfatide in beta cells

1. Sulfatide is present in the islets [2, 10].

2. Sulfatide recycles in the beta cells and it is here that the C16:0 isoform is predominately found [4].

3. Sulfatide interacts with insulin [13].

4. Sulfatide facilitates the exocytosis of insulin secretory granules, activates potassium channels and induces beta cell rest [18].

Thin layer chromatography revealed the antigen to be sulfatide [2, 10], and FACS sorting has indicated sulfatide attachment to the cell membrane [11]. Sulfatide is primarily produced by a recycling pathway in beta cells [4]. Desulphation takes place in lysosomes and is catalysed by arylsulphatase [12]; the GalCer formed is transported to the Golgi apparatus, where new sulphation by CST takes place [4]. Thus, like proinsulin, sulfatide is formed in the Golgi apparatus, and may even facilitate proinsulin folding [13]. Sulfatide also seems to be involved in the preservation of insulin crystals. In the absence of sulfatide these crystals are broken down after a couple of days, even under optimal in vitro conditions, whereas in its presence the crystals have a lifespan of up to 3 weeks [13]. Interestingly, insulin crystals are preserved by the C16:0 fatty acid isoform of sulfatide [14] which is found in the pancreas, but neither the long-chain isoform nor GalCer have this effect [13, 14]. Finally, there are strong indications that sulfatide facilitates the instant monomerisation of insulin, which takes place during the secretion of this hormone from the beta cells [13]. Thus, sulfatide seems to serve as a molecular chaperone for insulin—one of the first examples of a non-protein chaperone effect [15].
Fig. 2

Interactions of sulfatide in beta cells. The upper panel shows the ratio of the predominant sulfatide isoforms (C16:0 and C24:0) in the brain, pancreas and serum. The presence of sulfatide in the islets is demonstrated by staining with the monoclonal anti-sulfatide antibody Sulph I. The majority of sulfatide in the beta cells is synthesised via a recycling process [4] that involves several enzymatic steps: CGT adds galactose to the ceramide moiety, giving rise to GalCer, which is then sulphated by CST to form sulfatide. Sulphation takes place in the trans-Golgi apparatus, and sulfatide follows insulin trafficking into the secretory granules, as shown in the electron micrograph of human islets (beta cells) (lower panel, indicated by the black arrow). Sulfatide is either secreted together with insulin or transported to lysosomal structures (lower panel, indicated by the white arrow). In the lysosomes, sulfatide is degraded to GalCer by arylsulphatase A (ASA) and recycled to the cis-Golgi apparatus to serve as a sulfatide precursor [4]. Sulfatide assists proinsulin folding in the Golgi compartment and preserves insulin crystals in the secretory granules, as shown by the scanning electron micrographs (middle panel, left-hand insert). The micrographs show insulin crystals maintained at pH conditions comparable to those of secretory granules in the presence (top) or absence (bottom) of sulfatide. A model of insulin-sulfatide interactions at the level of secretion (where crystals first disintegrate into insulin hexamers, and then into the biologically active insulin monomer) has been proposed (middle panel, right-hand insert) [13]

Beta cells secrete insulin in a chain reaction, each succeeding the next in terms of secretion and subsequent rest. Regulation of this succession is unknown but each individual beta cell presumably requires a period of rest before its next first-phase insulin response. Insulin stimulates its own secretion via positive feedback [16], whereas C-peptide inhibits insulin secretion [17]. Sulfatide inhibits insulin release from glucose-activated islets in vitro in a similar fashion, activating potassium channels which subsequently close calcium channels, thereby mediating beta cell rest [18]. Use of diazoxide, or other pharmacological compounds that activate ATP-sensitive potassium channels in a manner similar to sulfatide, has been considered for the treatment of pre-type 1 [19] and type 2 diabetes [20] patients.

Sulfatide and type 1 diabetes

Sulfatide has been linked to type 1 diabetes (Text box: Sulfatide and type 1 diabetes).

Sulfatide and type 1 diabetes

1. Anti-sulfatide antibodies are present in type 1 diabetes [2].

2. Anti-sulfatide antibodies can be detected by ELISA [21].

3. Sulfatide inhibits the development of type 1 diabetes in NOD mice [23].

4. Sulfatide inhibits cytokine production [25, 26].

5. Sulfatide is a ligand for P- and L-selectin [28].

6. Sulfatide binds to CD1a, b, and c and stimulates the production of regulatory CD3+CD25+ T cells [34].

7. Sulfatide binds to CD1d [36].

Anti-sulfatide antibodies, detected using a conventional ELISA [21] are frequently present at diagnosis [2] and before the onset of diabetes [21]. Although antibodies to GAD, insulin and insulinoma-associated antigen-2 are well-established screening tests for diabetes [22], anti-sulfatide antibodies may also have prognostic value, and further refinement of analytical methods to achieve high throughput screening should enhance our understanding of their value in this respect. Anti-sulfatide antibodies are also found in neurological diseases such as Guillain–Barré syndrome [2], but those observed in patients with type 1 diabetes have a different specificity; seminolipid and SulphLacCer are labelled by anti-sulfatide antibodies from those with Guillain–Barré but not from those with diabetes (Fredman et al., unpublished observations). Furthermore, anti-sulfatide antibodies associated with type 1 diabetes inhibit insulin secretion and exocytosis from beta cells, whereas those from Guillain–Barré patients do not [18].

Treatment of non-obese diabetic (NOD) mice with sulfatide reduced the incidence of diabetes to 35%, as compared with 85% for the control animals [23]. Although many different treatments prevent diabetes in NOD mice, including numerous immunosuppressive agents, the few beta cell-related molecules that are effective in suppressing disease development [24] include GAD and insulin; sulfatide has a comparable effect of the same order of magnitude [23].

The T cell-dependent immune system is implicated in the development of type 1 diabetes, and it is therefore of interest that beta cells produce a peptide-based hormone, insulin, which is potentially immunogenic and an autoantigen in type 1 diabetes. Other endocrine cells that are not protected by the blood–brain barrier, as in the pituitary, produce hormones of low molecular weight that are not (or are only slightly) immunogenic. Beta cells require anti-inflammatory mechanisms for protection against accidental immune reactions; it may, therefore, be important that sulfatide has anti-inflammatory properties (Text box: Sulfatide and type 1 diabetes). T cell clones against beta cell epitopes and insulitis have been demonstrated in various animal models and in humans, and cytokines are involved in the maintenance of insulitis and may also cause direct beta cell damage. Sulfatide interferes with a number of these processes, as discussed below.

The role of sulfatide in cytokine production was investigated using unselected white blood cells from healthy donors. Sulfatide inhibits the production of IL-1, IL-6, IL-10 and TNF-α, whereas GalCer increases the secretion of IL-1, IL-6 and TNF-α [25, 26]. Similar changes were observed at the mRNA level [26]. In terms of isoforms, the C16:0 isoform of sulfatide was able to reduce the production of IL-1, IL-6, TNF-α and the chemokines MIP-1α and IL-8 by approximately 50%, whereas the C24:0 isoform was less effective at decreasing cytokine production [27].

Sulfatide has other anti-inflammatory properties. It binds to P- and L-selectin and, being a ligand of these molecules, it inhibits the adhesion of inflammatory blood cells and thereby impairs the migration of these cells from the blood [28]. This might be important with regard to the pathogenesis of type 1 diabetes, as blockade of L-selectin inhibits insulitis and prevents diabetes in NOD mice [29]. The selectin-mediated anti-inflammatory effect of sulfatide has been demonstrated in other inflammatory conditions, namely experimentally induced liver inflammation [30] and experimentally induced acute lung injuries [31].

Glycosphingolipid molecules cannot be presented by the MHC complex, which only binds peptides. However, given that some T cells recognise glycosphingolipids, these molecules must be presented somehow. It has now been established that presentation is achieved through the MHC-like CD1 molecules [32]. The CD1 molecule contains a groove with two large hydrophobic pockets that are able to anchor the lipid tails of a glycosphingolipid [33]. Human antigen-presenting cells, such as dendritic cells, can express five kinds of CD1 molecules. These are divided into two groups: group 1 includes CD1a, b, c and e, and group 2 comprises CD1d only. It has been demonstrated that CD1a, b and c are able to load sulfatide on the cell surface without proteasome processing, and prime T helper type 1 and T helper type 2 responses [34]. Sulfatide-stimulated T cells include CD3+CD25+ cells, which have regulatory properties [34]. The crystal structure of the CD1a-sulfatide complex has been determined [35].

Treatment of mice with sulfatide prevents antigen-induced experimental autoimmune encephalomyelitis, an animal model of human multiple sclerosis [36]. The mechanism seemed to involve an increase in the number of regulatory T cells, many of which express NK1.1. Interestingly, sulfatide had no effect in CD1d-deficient mice, indicating that the protective effect of sulfatide involved binding to this CD1 variant [36].

Only a few other ligands of CD1d are known, one of which is the mammalian, non-antigenic (β-) GalCer [37]. The most investigated ligand is α-GalCer, which is isolated from marine sponges [38]. When presented by dendritic cells, α-GalCer stimulates natural killer T (NKT) cells. NKT cells are CD1d-restricted T cells, which in humans express Vα24JαQ in their T cell receptor [39]; they are thought to play a regulatory role in autoimmune responses in vivo [40]. Both SJL and NOD mice, animal models of autoimmune diseases, have defects in NKT cell development and/or function [41, 42].

NKT cell numbers are reduced in humans with autoimmune diseases [43]. Studies using NK1.1 markers found decreased number of NKT cells in patients with type 1 diabetes [44]. Conversely, detection of NKT cells by tetramer CD1d/α-GalCer indicated no systemic reduction in cell numbers [45]. However, this does not exclude a relative deficiency of NKT cells within the relevant organ in situ, as α-GalCer-specific activation of NKT cells protects against diabetes in NOD mice [46, 47], providing strong evidence that CD1d-reactive NKT cells suppress autoreactive T cells. Similarly, overexpression of NKT cells protects Vα14Jα281 transgenic NOD mice against diabetes [48], whereas a shortage of NKT cells in CD1d knock-out mice leads to exacerbation of type 1 diabetes [49]. Finally, upregulation of CD1d expression within the beta cells restores the immunoregulatory function of NKT cells and prevents diabetes in NOD mice [50]. In the latter study the authors speculate as to the nature of the endogenous ligand that was loaded in the CD1d receptor. This might indeed have been sulfatide, which is present in the islet [4], can act as a ligand for CD1d [36], and inhibits (as with α-GalCer) diabetes development in the NOD mouse model [23].

Sulfatide and type 2 diabetes

Sulfatide might also be involved in type 2 diabetes (Text box: Sulfatide and type 2 diabetes).

Sulfatide and type 2 diabetes

1. Type 2 diabetes animal models lack C16:0 sulfatide [14].

2. Sulfatide mediates beta cell rest [18].

3. Sulfatide reduces the production of TNF-α [25].

4. Low serum concentrations of sulfatide and the presence of SulphLacCer are associated with insulin resistance and type 2 diabetes [51].

5. Treatment with sulfatide increases insulin secretion in Zucker fatty rats [54].

Insulin resistance is associated with low serum levels of sulfatide and/or the presence of another sulphated glycosphingolipid, SulphLacCer [51]. In this study, sera from 270 type 2 diabetic patients were analysed for sulfatide and SulphLacCer and compared with sera from a similar number of healthy controls, who were matched in terms of sex and age (mean age 65 years). For individuals in the lowest serum sulfatide tertile the odds ratio for type 2 diabetes was approximately 2.2 for both sexes, and for individuals with SulphLacCer in their serum (which is not usually present) the odds ratios were 1.7 and 7.6 for men and women, respectively [51]. These associations of type 2 diabetes with sulfatide and SulphLacCer were independent of each other and of other known classical diabetes risk factors.

Although the effect of sulfatide on insulin resistance [51] has not been explained, it could be partly mediated through inhibition of TNF-α [27], which is known to be present at elevated concentrations in type 2 diabetic patients and is associated with increased insulin resistance [52]. TNF-α is produced in fat cells and other tissues, and preliminary in vitro studies of human adipocytes have shown that sulfatide inhibits secretion of TNF-α and IL-6 by up to 40%, which is reflected by a corresponding decrease in levels of TNF-α mRNA.

At the beta cell level, sulfatide might suppress type 2 diabetes through activation of potassium channels, an effect mainly attributable to the C16:0 isoform of sulfatide [18]. The C16:0 isoform of sulfatide is present in substantial amounts in the pancreas of Lewis rats [4], BALB/c mice and humans [53], but ob/ob mice and db/db mice selectively lack this specific isoform of sulphatide [14]. In the Zucker rat, an animal model of type 2 diabetes, treatment with C16:0 sulfatide intraperitoneally twice a week almost doubled insulin secretion and improved the first-phase insulin response, as detected in a glucose tolerance test [54]. This is consistent with the effects of diazoxide and pharmacological potassium-channel openers, which improve insulin secretory responsiveness by mediating beta cell rest [20].

Notes

Acknowledgements

We thank B.-M. Rynmark, A. Roeske-Nielsen, J.-E. Månsson, and K.H. Jørgensen for helpful comments on the manuscript.

References

  1. 1.
    Fredman P, Vedeler CA, Nyland H, Aarli JA, Svennerholm L (1991) Antibodies in sera from patients with inflammatory demyelinating polyradiculoneuropathy react with ganglioside LM1 and sulphatide of peripheral nerve myelin. J Neurol 238:75–79CrossRefPubMedGoogle Scholar
  2. 2.
    Buschard K, Josefsen K, Horn T, Fredman P (1993) Sulphatide and sulphatide antibodies in insulin-dependent diabetes mellitus. Lancet 342:840CrossRefPubMedGoogle Scholar
  3. 3.
    Vos JP, Lopes-Cardozo M, Gadella BM (1994) Metabolic and functional aspects of sulfogalactolipids. Biochim Biophys Acta 1211:125–149PubMedGoogle Scholar
  4. 4.
    Fredman P, Mansson JE, Rynmark BM et al (2000) The glycosphingolipid sulfatide in the islets of Langerhans in rat pancreas is processed through recycling: possible involvement in insulin trafficking. Glycobiology 10:39–50CrossRefPubMedGoogle Scholar
  5. 5.
    Coetzee T, Li X, Fujita N et al (1996) Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene. Genomics 35:215–222CrossRefPubMedGoogle Scholar
  6. 6.
    Honke K, Tsuda M, Koyota S et al (2001) Molecular cloning and characterization of a human beta-Gal-3′-sulfotransferase that acts on both type 1 and type 2 (Gal beta 1-3/1-4GlcNAc-R) oligosaccharides. J Biol Chem 276:267–274Google Scholar
  7. 7.
    Coetzee T, Fujita N, Dupree J et al (1996) Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability. Cell 86:209–219CrossRefPubMedGoogle Scholar
  8. 8.
    Honke K, Hirahara Y, Dupree J et al (2002) Paranodal junction formation and spermatogenesis require sulfoglycolipids. Proc Natl Acad Sci U S A 99:4227–4232CrossRefPubMedGoogle Scholar
  9. 9.
    Fredman P, Mattsson L, Andersson K et al (1988) Characterization of the binding epitope of a monoclonal antibody to sulphatide. Biochem J 251:17–22PubMedGoogle Scholar
  10. 10.
    Buschard K, Josefsen K, Hansen SV et al (1994) Sulphatide in islets of Langerhans and in organs affected in diabetic late complications: a study in human and animal tissue. Diabetologia 37:1000–1006PubMedGoogle Scholar
  11. 11.
    Buschard K, Josefsen K, Rygaard J, Spitalnik SL (1991) Pancreatic islet-cell epitope recognized by an anti-sulphatide monoclonal antibody. APMIS 99:1151–1156PubMedGoogle Scholar
  12. 12.
    Stein C, Gieselmann V, Kreysing J et al (1989) Cloning and expression of human arylsulfatase A. J Biol Chem 264:1252–1259Google Scholar
  13. 13.
    Osterbye T, Jorgensen KH, Fredman P et al (2001) Sulfatide promotes the folding of proinsulin, preserves insulin crystals, and mediates its monomerization. Glycobiology 11:473–479CrossRefPubMedGoogle Scholar
  14. 14.
    Blomqvist M, Osterbye T, Mansson JE, Horn T, Buschard K, Fredman P (2003) Selective lack of the C16:0 fatty acid isoform of sulfatide in pancreas of type II diabetic animal models. APMIS 111:867–877CrossRefPubMedGoogle Scholar
  15. 15.
    Bogdanov M, Dowhan W (1999) Lipid-assisted protein folding. J Biol Chem 274:36827–36830Google Scholar
  16. 16.
    Kulkarni RN, Bruning JC, Winnay JN, Postic C, Magnuson MA, Kahn CR (1999) Tissue-specific knockout of the insulin receptor in pancreatic beta cells creates an insulin secretory defect similar to that in type 2 diabetes. Cell 96:329–339CrossRefPubMedGoogle Scholar
  17. 17.
    Toyota T, Abe K, Kudo M, Kimura K, Goto Y (1975) Inhibitory effects of synthetic rat C-peptide 1 on insulin secretion in the isolated perfused rat pancreas. Tohoku J Exp Med 117:79–83PubMedGoogle Scholar
  18. 18.
    Buschard K, Hoy M, Bokvist K et al (2002) Sulfatide controls insulin secretion by modulation of ATP-sensitive K(+)-channel activity and Ca(2+)-dependent exocytosis in rat pancreatic beta-cells. Diabetes 51:2514–2521PubMedGoogle Scholar
  19. 19.
    Skak K, Gotfredsen CF, Lundsgaard D, Hansen JB, Sturis J, Markholst H (2004) Improved beta-cell survival and reduced insulitis in a type 1 diabetic rat model after treatment with a beta-cell-selective K(ATP) channel opener. Diabetes 53:1089–1095PubMedGoogle Scholar
  20. 20.
    Carr RD, Brand CL, Bodvarsdottir TB, Hansen JB, Sturis J (2003) NN414, a SUR1/Kir6.2-selective potassium channel opener, reduces blood glucose and improves glucose tolerance in the VDF Zucker rat. Diabetes 52:2513–2518PubMedGoogle Scholar
  21. 21.
    Andersson K, Buschard K, Fredman P et al (2002) Patients with insulin-dependent diabetes but not those with non-insulin-dependent diabetes have anti-sulfatide antibodies as determined with a new ELISA assay. Autoimmunity 35:463–468CrossRefPubMedGoogle Scholar
  22. 22.
    Krischer JP, Cuthbertson DD, Yu L et al (2003) Screening strategies for the identification of multiple antibody-positive relatives of individuals with type 1 diabetes. J Clin Endocrinol Metab 88:103–108CrossRefPubMedGoogle Scholar
  23. 23.
    Buschard K, Hanspers K, Fredman P, Reich EP (2001) Treatment with sulfatide or its precursor, galactosylceramide, prevents diabetes in NOD mice. Autoimmunity 34:9–17PubMedGoogle Scholar
  24. 24.
    Atkinson MA, Leiter EH (1999) The NOD mouse model of type 1 diabetes: as good as it gets? Nat Med 5:601–604PubMedGoogle Scholar
  25. 25.
    Buschard K, Diamant M, Bovin LE, Mansson JE, Fredman P, Bendtzen K (1996) Sulphatide and its precursor galactosylceramide influence the production of cytokines in human mononuclear cells. APMIS 104:938–944PubMedGoogle Scholar
  26. 26.
    Bovin LF, Fredman P, Mansson JE, Buschard K, Bendtzen K (1999) In vitro production of cytokines is influenced by sulfatide and its precursor galactosylceramide. FEBS Lett 455:339–343CrossRefPubMedGoogle Scholar
  27. 27.
    Roeske-Nielsen A, Fredman P, Mansson JE, Bendtzen K, Buschard K (2004) Beta-galactosylceramide increases and sulfatide decreases cytokine and chemokine production in whole blood cells. Immunol Lett 91:205–211CrossRefPubMedGoogle Scholar
  28. 28.
    Suzuki Y, Toda Y, Tamatani T et al (1993) Sulfated glycolipids are ligands for a lymphocyte homing receptor, L-selectin (LECAM-1), binding epitope in sulfated sugar chain. Biochem Biophys Res Commun 190:426–434CrossRefPubMedGoogle Scholar
  29. 29.
    Yang XD, Karin N, Tisch R, Steinman L, McDevitt HO (1993) Inhibition of insulitis and prevention of diabetes in nonobese diabetic mice by blocking L-selectin and very late antigen 4 adhesion receptors. Proc Natl Acad Sci U S A 90:10494–10498PubMedGoogle Scholar
  30. 30.
    Kajihara J, Guoji Y, Kato K, Suzuki Y (1995) Sulfatide, a specific sugar ligand for L-selectin, blocks CCl4-induced liver inflammation in rats. Biosci Biotechnol Biochem 59:155–157PubMedGoogle Scholar
  31. 31.
    Mulligan MS, Miyasaka M, Suzuki Y et al (1995) Anti-inflammatory effects of sulfatides in selectin-dependent acute lung injury. Int Immunol 7:1107–1113PubMedGoogle Scholar
  32. 32.
    Porcelli SA, Modlin RL (1999) The CD1 system: antigen-presenting molecules for T cell recognition of lipids and glycolipids. Annu Rev Immunol 17:297–329CrossRefPubMedGoogle Scholar
  33. 33.
    Zeng Z, Castano AR, Segelke BW, Stura EA, Peterson PA, Wilson IA (1997) Crystal structure of mouse CD1: an MHC-like fold with a large hydrophobic binding groove. Science 277:339–345Google Scholar
  34. 34.
    Shamshiev A, Gober HJ, Donda A, Mazorra Z, Mori L, De Libero G (2002) Presentation of the same glycolipid by different CD1 molecules. J Exp Med 195:1013–1021CrossRefPubMedGoogle Scholar
  35. 35.
    Zajonc DM, Elsliger MA, Teyton L, Wilson IA (2003) Crystal structure of CD1a in complex with a sulfatide self antigen at a resolution of 2.15 Å. Nat Immunol 4:808–815CrossRefPubMedGoogle Scholar
  36. 36.
    Jahng A, Maricic I, Aguilera C, Cardell S, Halder RC, Kumar V (2004) Prevention of autoimmunity by targeting a distinct, noninvariant CD1d-reactive T cell population reactive to sulfatide. J Exp Med 199:947–957CrossRefPubMedGoogle Scholar
  37. 37.
    Naidenko OV, Maher JK, Ernst WA, Sakai T, Modlin RL, Kronenberg M (1999) Binding and antigen presentation of ceramide-containing glycolipids by soluble mouse and human CD1d molecules. J Exp Med 190:1069–1080CrossRefPubMedGoogle Scholar
  38. 38.
    Burdin N, Brossay L, Koezuka Y et al (1998) Selective ability of mouse CD1 to present glycolipids: alpha-galactosylceramide specifically stimulates V alpha 14+ NK T lymphocytes. J Immunol 161:3271–3281PubMedGoogle Scholar
  39. 39.
    Kent SC, Hafler DA, Strominger JL, Wilson SB (1999) Noncanonical Vα24JαQ T cells with conservative alpha chain CDR3 region amino acid substitutions are restricted by CD1d. Hum Immunol 60:1080–1089CrossRefPubMedGoogle Scholar
  40. 40.
    Wilson SB, Delovitch TL (2003) Janus-like role of regulatory iNKT cells in autoimmune disease and tumour immunity. Nat Rev Immunol 3:211–222CrossRefPubMedGoogle Scholar
  41. 41.
    Yoshimoto T, Bendelac A, Hu-Li J, Paul WE (1995) Defective IgE production by SJL mice is linked to the absence of CD4+, NK1.1+ T cells that promptly produce interleukin 4. Proc Natl Acad Sci U S A 92:11931–11934PubMedGoogle Scholar
  42. 42.
    Gombert JM, Herbelin A, Tancrede-Bohin E, Dy M, Carnaud C, Bach JF (1996) Early quantitative and functional deficiency of NK1+-like thymocytes in the NOD mouse. Eur J Immunol 26:2989–2998PubMedGoogle Scholar
  43. 43.
    van der Vliet HJ, von Blomberg BM, Nishi N et al (2001) Circulating Vα24+ Vβ11+ NKT cell numbers are decreased in a wide variety of diseases that are characterized by autoreactive tissue damage. Clin Immunol 100:144–148CrossRefPubMedGoogle Scholar
  44. 44.
    Wilson SB, Kent SC, Patton KT et al (1998) Extreme Th1 bias of invariant Vα24JαQ T cells in type 1 diabetes. Nature 391:177–181PubMedGoogle Scholar
  45. 45.
    Lee PT, Putnam A, Benlagha K, Teyton L, Gottlieb PA, Bendelac A (2002) Testing the NKT cell hypothesis of human IDDM pathogenesis. J Clin Invest 110:793–800PubMedGoogle Scholar
  46. 46.
    Hong S, Wilson MT, Serizawa I et al (2001) The natural killer T-cell ligand alpha-galactosylceramide prevents autoimmune diabetes in non-obese diabetic mice. Nat Med 7:1052–1056PubMedGoogle Scholar
  47. 47.
    Sharif S, Arreaza GA, Zucker P et al (2001) Activation of natural killer T cells by alpha-galactosylceramide treatment prevents the onset and recurrence of autoimmune Type 1 diabetes. Nat Med 7:1057–1062CrossRefPubMedGoogle Scholar
  48. 48.
    Lehuen A, Lantz O, Beaudoin L et al (1998) Overexpression of natural killer T cells protects Vα14-Jα281 transgenic nonobese diabetic mice against diabetes. J Exp Med 188:1831–1839PubMedGoogle Scholar
  49. 49.
    Shi FD, Flodstrom M, Balasa B et al (2001) Germ line deletion of the CD1 locus exacerbates diabetes in the NOD mouse. Proc Natl Acad Sci U S A 98:6777–6782CrossRefPubMedGoogle Scholar
  50. 50.
    Falcone M, Facciotti F, Ghidoli N et al (2004) Up-regulation of CD1d expression restores the immunoregulatory function of NKT cells and prevents autoimmune diabetes in nonobese diabetic mice. J Immunol 172:5908–5916PubMedGoogle Scholar
  51. 51.
    Buschard K, Fredman P, Bøg-Hansen E et al (2005) Low serum concentration of sulfatide and presence of sulfated lactosylceramide are associated with type 2 diabetes. Diabet Med 22:1190–1198CrossRefPubMedGoogle Scholar
  52. 52.
    Miyazaki Y, Pipek R, Mandarino LJ, DeFronzo RA (2003) Tumor necrosis factor alpha and insulin resistance in obese type 2 diabetic patients. Int J Obes Relat Metab Disord 27:88–94CrossRefPubMedGoogle Scholar
  53. 53.
    Blomqvist M, Kaas A, Mansson JE et al (2003) Developmental expression of the type I diabetes related antigen sulfatide and sulfated lactosylceramide in mammalian pancreas. J Cell Biochem 89:301–310CrossRefPubMedGoogle Scholar
  54. 54.
    Blomqvist M, Carrier M, Andrews T et al (2005) In vivo administration of the C16:0 fatty acid isoform of sulfatide increases pancreatic sulfatide and enhances glucose-stimulated insulin secretion in Zucker fatty (fa/fa) rats. Diabetes Metab Res Rev 21:158–166CrossRefPubMedGoogle Scholar

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© Springer-Verlag 2005

Authors and Affiliations

  • K. Buschard
    • 1
  • M. Blomqvist
    • 2
  • T. Osterbye
    • 1
  • P. Fredman
    • 2
  1. 1.Bartholin InstituteCopenhagen University HospitalCopenhagenDenmark
  2. 2.Institute of Clinical Neuroscience, Experimental Neuroscience SectionThe Sahlgrenska Academy at Göteborg University, Sahlgrenska University HospitalMölndalSweden

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