Theoretical and Applied Genetics

, Volume 104, Issue 2–3, pp 505–512 | Cite as

Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies

  • S. Siljak-Yakovlev
  • M. Cerbah
  • J. Coulaud
  • V. Stoian
  • S. C. Brown
  • V. Zoldos
  • S. Jelenic
  • D. Papes

Abstract 

Two closely related spruces, Picea abies and Picea omorika, a Balkan paleoendemic species, often share habitats, yet never hybridize in nature. The present study adresses their characteristics such as nuclear DNA content, base composition, heterochromatin and rDNA pattern. The genome size of P. abies was 10% larger than that of P. omorika when assessed by flow cytometry, respectively 2C=37.2 pg and 33.8 pg; although when estimated as total chromosome length it was virtually the same. The heterochromatin Chromomycin-A (CMA)/ DAPI fluorochrome banding patterns of both P. abies and P. omorika are given here for the first time. Simultaneous FISH (fluorescent in situ hybridization) using 18S-26S and 5S rDNA probes revealed 16 18S rDNA sites in P. omorika, 12 18S rDNA sites in P. abies, and a single 5S rDNA locus in both species. The genomes have about 41% GC. The number and position of CMA/DAPI bands and rDNA loci provide good chromosome markers to clarify the karyotypes of the two species.

Keywords  Picea Fluorochrome banding Flow cytometry In situ hybridization 18S-5.8S-26S and 5S rDNA Nucleolar organizer region 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • S. Siljak-Yakovlev
    • 1
  • M. Cerbah
    • 1
  • J. Coulaud
    • 1
  • V. Stoian
    • 2
  • S. C. Brown
    • 3
  • V. Zoldos
    • 4
  • S. Jelenic
    • 4
  • D. Papes
    • 4
  1. 1.Laboratoire d’Ecologie, Systématique et Evolution, CNRS UPRESA 8079, Université Paris XI, Bât 360, 91405 Orsay, France e-mail: sonia.yakovlev@esv.u-psud.fr Tel.: +33-1-69-15-46-98, Fax: +33-1-69-15-46-97FR
  2. 2.Department of Genetics, University of Bucharest, Bucharest, RomaniaRO
  3. 3.Institut des Sciences Végétales, CNRS UPR 40, 91198 Gif-sur-Yvette, FranceFR
  4. 4.Department of Molecular Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaHR

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