Theoretical and Applied Genetics

, Volume 103, Issue 8, pp 1129–1137

Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust

  • B. Stirling
  • G. Newcombe
  • J. Vrebalov
  • I. Bosdet
  • H.D. Bradshaw Jr.

DOI: 10.1007/s001220100721

Cite this article as:
Stirling, B., Newcombe, G., Vrebalov, J. et al. Theor Appl Genet (2001) 103: 1129. doi:10.1007/s001220100721

Abstract 

A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902) Populus trichocarpa×P. deltoides (T×D) mapping pedigree segregating 1:1 for rust resistance and susceptibility. The high-resolution linkage map developed around the MXC3 locus contains 19 AFLP markers and spans a genetic distance of 2.73 cM. Of the 19 AFLP markers, seven were found to co-segregate with the locus. One co-segregating AFLP marker, CCG.GCT_01, was converted to an STS marker (BVS1) and used to identify a physical contig of overlapping BAC clones from the MXC3 region. Genetic and physical mapping of markers isolated from the BAC contig failed to delimit the MXC3 locus within a 300-kb interval defined by the overlapping BAC clones. This result indicates a >25-fold reduction in recombination frequency in the MXC3 region compared to the average rate of recombination for the Populus genome.

Keywords Map-based cloning Hybrid poplar Plant disease resistance 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • B. Stirling
    • 1
  • G. Newcombe
    • 2
  • J. Vrebalov
    • 3
  • I. Bosdet
    • 4
  • H.D. Bradshaw Jr.
    • 1
  1. 1.Center for Urban Horticulture, Box 354115, 3501 NE 41st St., University of Washington, Seattle, WA 98195-4115, USA e-mail: toby@u.washington.edu Tel.: +206 616-1796, Fax: +206 685-2692US
  2. 2.Department of Forest Resources, University of Idaho, Moscow, Idaho 83844-1183, USAUS
  3. 3.Boyce Thompson Institute of Plant Research, Tower Road, Cornell University Campus, Ithaca, NY 14853, USAUS
  4. 4.Genome Sequence Centre, B.C. Cancer Research Centre, Vancouver V5Z-4E6, B.C., CanadaCA

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