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Theoretical and Applied Genetics

, Volume 103, Issue 2–3, pp 212–218 | Cite as

Distribution of rDNA loci in the genus Glycine Willd.

  • R. J. Singh
  • H. H. Kim
  • T. Hymowitz
Original Article

Abstract  

The objective of this study was to examine the distribution of rDNA loci in the genus Glycine Willd. by fluorescent in situ hybridization (FISH) using the internal transcribed spacer (ITS) region of nuclear ribosomal DNA as a probe. The hybridized rDNA probe produced two distinct yellow signals on reddish chromosomes representing two NORs in 16 diploid (2n=40) species. Aneudiploid (2n=38) and aneutetraploid (2n=78) Glycine tomentella Hayata also exhibited two rDNA sites. However, the probe hybridized with four chromosomes as evidenced by four signals in two diploid species (Glycine curvata Tind. and Glycine cyrtoloba Tind.) and tetraploid (2n=80) G. tabacina (Labill.) Benth. and G. tomentella. Synthesized amphiploids (2n=80) of Glycine canescens F. J. Herm. (2n=40) and the 40-chromosome G. tomentella also showed four signals. This study demonstrates that the distribution of the rDNA gene in the 16 Glycine species studied is highly conserved and that silence of the rDNA locus may be attributed to amphiplasty during diploidization and speciation.

Keywords Soybean Glycine rDNA Fluorescent in situ hybridization Polyploidy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • R. J. Singh
    • 1
  • H. H. Kim
    • 2
  • T. Hymowitz
    • 1
  1. 1.Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA e-mail: r-singh@uiuc.eduUS
  2. 2.Department of Biology, Korean Sahmyook University, Seoul, 139–742, South KoreaKR

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