Theoretical and Applied Genetics

, Volume 102, Issue 6–7, pp 810–814 | Cite as

Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH

  • R. Nakamura
  • S. Kitamura
  • M. Inoue
  • N. Ohmido
  • K. Fukui
Original Paper

Abstract 

Prometaphase cells were used to analyze the karyotype of Nicotiana kawakamii Y. Ohashi by means of sequential Giemsa/CMA/DAPI staining and multicolor fluorescence in situ hybridization with 5S and 18S rDNA. Observation of the DAPI-stained prometaphase spreads indicated that N. kawakamii had six pairs of large chromosomes, one pair of medium-sized chromosomes and five pairs of small chromosomes. The six pairs of large chromosomes possessed remarkable DAPI bands, and each could be identified from both the DAPI banding pattern and the length of the short arm. The DAPI banding pattern was approximately identical to the CMA and Giemsa banding patterns. Hybridization signals of the 18S rDNA probe were detected on two pairs of large chromosomes. In addition, two pairs of small chromosomes were identified based on the position of the 5S rDNA signals. An idiogram of N. kawakamii chromosomes was produced based on DAPI bands and rDNA loci.

Keywords Nicotiana kawakamii Y. Ohashi Karyotype Giemsa/CMA/DAPI staining rDNA FISH Quantitative idiogram 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • R. Nakamura
    • 1
  • S. Kitamura
    • 1
  • M. Inoue
    • 1
  • N. Ohmido
    • 2
  • K. Fukui
    • 2
  1. 1.Laboratory of Plant Breeding Science, Faculty of Agriculture, Kyoto Prefectural University, Sakyo, Kyoto 606-8522, Japan Fax: +81-75-7035603JP
  2. 2.Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu 943-0193, Japan, Present address: K. Fukui, Department of Biotechnology, Faculty of Engineering, Graduate School of Osaka University, 2-1 Yamada-Oka, Suita 565-0871, JapanJP

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