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Theoretical and Applied Genetics

, Volume 102, Issue 4, pp 645–650 | Cite as

Molecular relatedness of the polygalacturonase-inhibiting protein genes in Eucalyptus species

  • P. M. Chimwamurombe
  • A.-M. Botha
  • M. J. Wingfield
  • B. D. Wingfield
Original Article

Abstract 

Plants produce polygalacturonase-inhibiting proteins (PGIPs) as part of their defense against disease. PGIPs have leucine-rich motifs, a characteristic shared by many proteins involved in plant resistance against pathogens. The objective of this study was to clone and analyse the partial sequences of the pgip genes from five selected commercially important Eucalyptus species. Genomic DNA from E. grandis, E. urophylla, E. camaldulensis, E. nitens and E. saligna was isolated from young leaves and used as the template in PCR reactions. Primers PC1, previously described, and Per3, developed in this study, were used in a degenerate PCR reaction to amplify a pgip fragment. A PCR fragment of 909 bp was amplified from each Eucalyptus spp., cloned and sequenced. The Eucalyptus pgip genes were highly conserved (98–100% identity). Analysis of the deduced amino-acid sequences revealed high similarities (44–94%) with other known PGIPs. In general, PGIPs have high homologies within genera as is the case in the genus Citrus. These observations strengthen the belief that PGIP plays an important role in plants.

Keywords Eucalyptus Leucine-rich repeats PGIPs Signal transduction Resistance 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • P. M. Chimwamurombe
    • 1
  • A.-M. Botha
    • 1
  • M. J. Wingfield
    • 1
  • B. D. Wingfield
    • 1
  1. 1.Forest and Molecular Biology Co-operative Programme, Department of Genetics, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa e-mail: Brenda.Wingfield@fabi.up.ac.za Tel.: +27-12-420-3946, Fax: +27-12-420-3947ZA

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