Theoretical and Applied Genetics

, Volume 102, Issue 4, pp 555–563

Characterisation of disease resistance gene-like sequences in Brassica oleracea L.

  • J. G. Vicente
  • G. J. King
Original Article

DOI: 10.1007/s001220051682

Cite this article as:
Vicente, J. & King, G. Theor Appl Genet (2001) 102: 555. doi:10.1007/s001220051682


Several cloned disease resistance genes from a wide range of plant species are known to share conserved regions with similar structural motifs. Degenerate primers based on conserved sequences of the nucleotide binding site of the genes RPS2, N and L6 were used for polymerase chain reaction (PCR) amplification from genomic DNA of two doubled haploid lines of Brassica oleracea. Sequences of amplified products were highly variable, but most of them showed similarity to known disease resistance genes, including RPS5, RPS2 and N, and to disease resistance gene-like sequences (RGLs) from different species. Primers based on B. oleracea sequences amplified five groups of RGLs. Products were mapped through cleaved amplified polymorphic sequence assays onto four different linkage groups of B. oleracea. PCR amplification from cDNA and allele analysis indicated that four locus-specific RGL fragments are expressed in cauliflower. Screening of a B. oleracea bacterial artificial chromosome library (BAC) with four B. oleracea RGL probes identified a small number of clones, suggesting that the four RGLs may not be highly copied. Screening of a BAC library of A. thaliana with the same probes identified clones that mapped onto four different chromosomes. These map positions correspond to known disease resistance loci of A. thaliana.

Keywords Brassica Candidate genes Genetic mapping Nucleotide binding site-leucine rich repeat (NBS-LRR) Degenerate oligonucleotides 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • J. G. Vicente
    • 1
  • G. J. King
    • 1
  1. 1.Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK e-mail:

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