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Theoretical and Applied Genetics

, Volume 102, Issue 2–3, pp 369–374 | Cite as

Biochemical genetics of glucosinolate modification in Arabidopsis and Brassica

  • C. Hall
  • D. McCallum
  • A. Prescott
  • R. Mithen
Original Paper

Abstract 

Fine mapping of the glucosinolate biosynthesis gene OHP, which regulates the conversion of 3-methylsulphinylpropyl to 3-hydroxypropyl glucosinolate, in an Arabidopsis thaliana Columbia × Landsberg erecta RI line population positioned the gene within 54 kb of DNA on chromosome IV. Sequence data identified a family of genes encoding 2-oxoglutarate-dependent dioxygenases in this region. A probe based on these genes co-segregated with ALK in Brassica oleracea,a gene regulating the synthesis of alkenyl glucosinolates. The reactions catalysed by the OHP and ALK enzymes utilise similar substrates and may have a common mechanism. Thus, these dioxygenases are prime candidates for controlling the side chain modification of glucosinolates.

Keywords Arabidopsis thaliana Brassica oleracea Glucosinolates 2-Oxoglutarate dependent dioxygenases 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • C. Hall
    • 1
  • D. McCallum
    • 2
  • A. Prescott
    • 1
  • R. Mithen
    • 1
  1. 1.John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH Fax: +44(0)1603 259882 e-mail: Richard.Mithen@bbsrc.ac.ukGB
  2. 2.Max Planck Institute of Chemical Ecology, Tatzendpromenade 1a, 07745 Jena, GermanyDE

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