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Theoretical and Applied Genetics

, Volume 101, Issue 8, pp 1202–1206 | Cite as

Development of STS markers closely linked to the Ppd-H1 photoperiod response gene of barley (Hordeum vulgare L.)

  • L. Decousset
  • S. Griffiths
  • R. P. Dunford
  • N. Pratchett
  • D. A. Laurie
Original Article

Abstract 

A BC2 population of 353 plants segregating for the Ppd-H1 photoperiod response gene was developed from a cross between the winter barley ’Igri’ and the spring barley ’Triumph.’ Bulk segregant analysis identified six AFLP markers closely linked to the Ppd–H1 gene and three strongly amplified AFLP bands that mapped 0.8-cM distal, 0.6-cM proximal and 2.3-cm proximal to Ppd-H1 were cloned and sequenced. Southern-blot analysis showed that the cloned fragments were single-copy sequences in ’Igri’, the variety from which they were derived. Two of the sequences were absent from ’Triumph’ while the third detected a single-copy sequence. The cloned fragments were used to design specific sequence tagged site (STS) primer pairs to assist in the construction of a high-resolution map of the Ppd-H1 region.

Keywords Amplified fragment length polymorphism (AFLP) Barley (Hordeum vulgare) Photoperiod Mapping Ppd-H1 Sequence tagged site (STS) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • L. Decousset
    • 1
  • S. Griffiths
    • 1
  • R. P. Dunford
    • 1
  • N. Pratchett
    • 1
  • D. A. Laurie
    • 1
  1. 1.John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, FranceGB

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