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Theoretical and Applied Genetics

, Volume 101, Issue 8, pp 1173–1179 | Cite as

Characterization of Aegilops uniaristata chromosomes by comparative DNA marker analysis and repetitive DNA sequence in situ hybridization

  • N. Iqbal
  • S. M. Reader
  • P. D. S. Caligari
  • T. E. Miller
Original Article

Abstract 

RFLP analyses were performed on wheat-Aegilops uniaristata Vis. addition and translocation lines to confirm the identity of added N-genome chromosomes. Complete 1N, 3N, 4N, 5N and 7N chromosome additions were identified, while the complete long arm and only part of the short arm was identified for chromosome 2N. There were no wheat-like 4/5 and 4/7 translocations in the Ae. uniaristata chromosomes. Chromosome 3N carried an asymmetric pericentric inversion, and the translocation line was a product of centric fusion between the long arms of chromosomes 3B and 3N. Chromosome-specific RAPD and microsatellite markers were also identified for all the added Ae. uniaristata chromosomes available in this set of addition lines. A new genomic in situ hybridization protocol combining pre-annealing of probe and blocking DNA and prehybridization with blocking DNA was developed to differentiate the very closely related genomes of Ae. uniaristata and wheat. Hybridization sites for the repetitive DNA sequences pAs1, pSc119.2 and pTa71 were identified on the N-genome chromosomes of Ae. uniaristata using the fluorescent in situ hybridization technique. Results showed deviation from the previously published ideogram of this species. A new ideogram, which shows the hybridization sites for the above sequences, was produced in which the chromosomes are arranged according to their homoeologous group.

Key words Addition lines Aegilops uniaristata In situ hybridization Karyotype Repetitive DNA sequences RFLP analysis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • N. Iqbal
    • 1
  • S. M. Reader
    • 1
  • P. D. S. Caligari
    • 2
  • T. E. Miller
    • 1
  1. 1.John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UKGB
  2. 2.Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UKGB

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