Theoretical and Applied Genetics

, Volume 101, Issue 7, pp 1100–1108 | Cite as

RFLP mapping of manganese efficiency in barley

  • M. A. Pallotta
  • R. D. Graham
  • P. Langridge
  • D. H. B. Sparrow
  • S. J. Barker
Original Article

Abstract 

In many cropping regions of the world, yield is limited by the availability of micronutrients, and micronutrient-efficient cultivars provide a yield advantage. Traditional methods of testing cultivars for micronutrient efficiency are time-consuming and laborious. Molecular markers linked to loci controlling micronutrient efficiency will allow more rapid and efficient selection and introgression of these traits than is currently possible. Using a pot-based bioassay and bulked segregant analysis of an F2 population, we have identified several RFLPs (grouped distally on chromosome 4HS) linked to a locus for manganese efficiency in barley. This manganese efficiency locus has been designated Mel1. Pot bioassay analysis of intercrosses suggests that three useful sources of manganese efficiency are likely to be allelic at the Mel1 locus. Field evaluation of marker selected F4 progeny supports the major role of Mel1 in the genetic control of manganese efficiency. Adoption of marker assisted selection for this trait in the Southern Australian barley breeding program has occurred. This has been facilitated by the demonstration that the Mel1 allele of Amagi Nijo can be distinguished from 95 other locally useful varieties and breeder’s lines on the basis of RFLPs identified by just two molecular markers.

Key words Barley Manganese efficiency RFLP mapping Marker-assisted Selection Plant nutrition 4HS 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. A. Pallotta
    • 1
  • R. D. Graham
    • 1
  • P. Langridge
    • 1
  • D. H. B. Sparrow
    • 1
  • S. J. Barker
    • 2
  1. 1.Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.auAU
  2. 2.Faculty of Agriculture, The University of Western Australia, Nedlands, Western Australia, Australia 6907AU

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