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Theoretical and Applied Genetics

, Volume 101, Issue 4, pp 652–660 | Cite as

Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars

  • K. Pillen
  • A. Binder
  • B. Kreuzkam
  • L. Ramsay
  • R. Waugh
  • J. Förster
  • J. Léon
Original paper

Abstract 

By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL microsatellites were mapped either to the interspecific barley map Lerche×BGRC41936 (L×41), the Igri×Franka map (I×F, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.

Key words Barley Microsatellites Linkage map Genetic similarity (GS) Polymorphism information content (PIC) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. Pillen
    • 1
  • A. Binder
    • 1
  • B. Kreuzkam
    • 1
  • L. Ramsay
    • 2
  • R. Waugh
    • 2
  • J. Förster
    • 3
  • J. Léon
    • 1
  1. 1.Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.deDE
  2. 2.Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, Scotland, UKGB
  3. 3.Saaten-Union Resistenzlabor GmbH, Hovedisser Str. 92, D-33818 Leopoldshöhe, GermanyDE

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