Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris
- 58 Downloads
Interspecific hybrids were produced by crosses between the inbred lines of B. campestris and B. alboglabra, and were backcrossed twice to B. campestris. Genetical constitutions of the BC2 plants were analyzed by RAPD (random amplified polymorphic DNA), flow cytometry and cytological observations. By using 140 arbitrary primers, a total of 137 polymorphic bands were obtained and 125 were found to be specific to B. alboglabra. Based on the presence and absence of the specific RAPD markers of B. alboglabra, 13 synteny groups were constructed. The number of markers in each synteny group was found to be different and varied from 2 to 28. This reflects the difference in the degree of genetic variability among the B. alboglabra chromosomes from those of B. campestris. Losses or gains of RAPD markers were observed frequently in most of the synteny groups, which indicated the occurrence of chromosome translocations and/or deletions in the chromosomes of B. alboglabra. In a population of 40 BC2 plants, chromosome transmission rates were analyzed by using the RAPD markers in each synteny group. Most of the chromosomes of the synteny groups were transmitted with rates of 0.37–0.68. An extremely high transmission rate, 0.98, was however observed in one of the synteny groups. Inheritance data of the synteny groups revealed relationships among themselves. The plants lacking the RAPD markers of two synteny groups tended to lose others belonging to the rest of the synteny groups, indicating the effects of these groups on the transmission of B. alboglabra chromosomes to the B. campestris background.
Unable to display preview. Download preview PDF.