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Theoretical and Applied Genetics

, Volume 101, Issue 3, pp 407–414 | Cite as

Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding

  • X. Q. Huang
  • S. L. K. Hsam
  • F. J. Zeller
  • G. Wenzel
  • V. Mohler

Abstract 

Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.

Keywords AFLPs Bulked segregant analysis Marker-assisted selection Microsatellites Powdery mildew resistance Triticum aestivum 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • X. Q. Huang
    • 1
  • S. L. K. Hsam
    • 1
  • F. J. Zeller
    • 1
  • G. Wenzel
    • 1
  • V. Mohler
    • 1
  1. 1.Technische Universität München, Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Alte Akademie 12, D-85350 Freising-Weihenstephan, Germany e-mail: mohler@weihenstephan.de Fax: +49 8161 715173 Present address: X.Q. Huang, Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, GermanyDE

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