The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.
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Received: 19 October 1999 / Accepted: 16 December 1999
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Garland, S., Lewin, L., Blakeney, A. et al. PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.). Theor Appl Genet 101, 364–371 (2000). https://doi.org/10.1007/s001220051492
- Keywords Fragrance
- PCR marker
- Oryza sativa