Theoretical and Applied Genetics

, Volume 101, Issue 3, pp 364–371 | Cite as

PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.)

  • S. Garland
  • L. Lewin
  • A. Blakeney
  • R. Reinke
  • R. Henry
Original Article

Abstract 

The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.

Keywords Fragrance Rice PCR marker Oryza sativa 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • S. Garland
    • 1
  • L. Lewin
    • 2
  • A. Blakeney
    • 3
  • R. Reinke
  • R. Henry
    • 1
  1. 1.Centre for Plant Conservation Genetics, Southern Cross University (SCU), Lismore, NSW, 2480, Australia+61-2 66222080AU
  2. 2.Yanco Agricultural Institute, NSW Agriculture, Yanco, NSW, 2703, AustraliaAU
  3. 3.Grains Processing, BRI Australia Ltd, North Ryde, NSW, 2113, AustraliaAU

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