Theoretical and Applied Genetics

, Volume 101, Issue 1–2, pp 286–291 | Cite as

Mapping of QTLs conferring resistance to bacterial leaf streak in rice

  • D. Tang
  • W. Wu
  • W. Li
  • H. Lu
  • A. J. Worland
Original Paper

Abstract 

A large F2 and a RI population were separately derived from a cross between two indica rice varieties, one of which was highly resistant to bacterial leaf streak (BLS) and the other highly susceptible. Following artificial inoculation of the RI population and over 2 years of testing, 11 QTLs were mapped by composite interval mapping (CIM) on six chromosomes. Six of the QTLs were detected in both seasons. Eight of the QTLs were significant following stepwise regression analysis, and of these, 5 with the largest effects were significant in both seasons. The detected QTLs explained 84.6% of the genetic variation in 1997. Bulked segregant analysis (BSA) of the extremes of the F2 population identified 3 QTLs of large effect. The 3 QTLs were dentical to 3 of the 5 largest QTLs detected by CIM. The independent detection of the same QTLs using two methods of analysis in separate mapping populations verifies the existence of the QTLs for BLS and provides markers to ease their introduction into elite varieties.

Key words Rice Bacterial leaf streak Mapping Bulked segregant analysis QTL 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • D. Tang
    • 1
  • W. Wu
    • 1
  • W. Li
    • 1
  • H. Lu
    • 1
  • A. J. Worland
    • 2
  1. 1.College of Crop Sciences, Fujian Agricultural University, Fuzhou, Fujian, China Fax: +86-591-3744744 e-mail: wuwr@fjau.edu.cnCN
  2. 2.Cereals Research Department, John Innes Centre, Norwich, NR4 7UH, UKGB

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