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Theoretical and Applied Genetics

, Volume 101, Issue 1–2, pp 51–58 | Cite as

Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines

  • B. Friebe
  • L. L. Qi
  • S. Nasuda
  • P. Zhang
  • N. A. Tuleen
  • B. S. Gill
Original Paper

Abstract 

Aegilops speltoides Tausch (2n = 2x = 14, SS) is considered as the closest living relative of the B and G genomes of polyploid wheats. A complete set of Triticumaestivum L. cv Chinese Spring-Ae. speltoides whole chromosomes and seven telosomic addition lines was established. A low pairing accession was selected for the isolation of the chromosome addition lines. Except for chromosomes 3S and 6S, which are presently only available as monosomic additions, all other lines were recovered as disomic or ditelosomic additions. The individual Ae. speltoides chromosomes isolated in the wheat background were assayed for their genetic effects on plant phenotype and cytologically characterized in terms of chromosome length, arm ratio, distribution of marker C-bands, and FISH sites using a Ae. speltoides-specific repetitive element, Gc1R-1, as a probe. The homoeology of the added Ae. speltoides chromosomes was established by using a standard set of RFLP probes. No chromosomal rearrangements relative to wheat were detected.

Key words Triticum aestivum Aegilops speltoides Chromosome addition C-banding In situ hybridization RFLP 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • B. Friebe
    • 1
  • L. L. Qi
    • 1
  • S. Nasuda
    • 2
  • P. Zhang
    • 1
  • N. A. Tuleen
    • 3
  • B. S. Gill
    • 1
  1. 1.Department of Plant Pathology, Wheat Genetics Resource Center, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506–5502. USA Fax: +1-785-532-5692 e-mail: friebe@ksu.eduUS
  2. 2.Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606–01, JapanJP
  3. 3.Department of Soil and Crop Sciences, Texas A & M University, College Station, TX 77843–2474, USAUS

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