Theoretical and Applied Genetics

, Volume 100, Issue 5, pp 665–669 | Cite as

Detection of 5S and 25S rRNA genes in Sinapis alba, Raphanus sativus and Brassica napus by double fluorescence in situ hybridization

  • O. Schrader
  • H. Budahn
  • R. Ahne
Original

Abstract 

Different ribosomal RNA (5S and 25S) genes were investigated simultaneously by fluorescence in situ hybridization (FISH) in Sinapis alba, Raphanus sativus and Brassica napus. The chromosomes of S. alba carried four 5S and six 25S gene sites, and those of R. sativus four sites of each gene, respectively. These two species have one chromosome pair with both rDNA genes; the two are closely located on a short arm of S. alba, while in R. sativus one is distal on the short arm (5S) and the other more proximal on the long arm (25S). In B. napus we have confirmed 12sites of 25S rDNA. The detection of 5S rDNA genes revealed 14 signals on 12 chromosomes. Of these, six chromosomes had signals for both rDNA genes. The FISH with 5S rDNA probes detected two sites closely adjacent in four chromosomes of B napus. These results are discussed in relation to a probable homoeologous chromosome pair in B. oleracea.

Key words Brassica napus Sinapis alba Raphanus sativus FISH 5S rDNA genes 25S rDNA genes 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • O. Schrader
    • 1
  • H. Budahn
    • 1
  • R. Ahne
    • 1
  1. 1.Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255DE

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