Theoretical and Applied Genetics

, Volume 99, Issue 3–4, pp 705–710 | Cite as

Average effect of a mutation in lignin biosynthesis in loblolly pine

  • R. L. Wu
  • D. L. Remington
  • J. J. MacKay
  • S. E. McKeand
  • D. M. O’Malley
Article

Abstract 

Cinnamyl alcohol dehydrogenase (CAD, E.C. 1.1.1.195) is a monolignol biosynthetic enzyme that catalyzes the final step of lignin subunit biosynthesis in higher plants. Recently, a mutant allele of the cad gene, cad-n1, encoding for the CAD enzyme, was discovered in loblolly pine. By reducing the expression of the cad gene, this mutant has a decreased lignin content and major changes in the lignin composition in wood. In this study, we found that the substitution of a wild-type allele by cad-n1 was associated with a significant effect on 2nd-year shoot elongation in a half-sib family of loblolly pine (designated family 7–1037). The average effect of cad-n1 appeared to increase with tree growth and was greater for stem radial growth than height growth. An increase of 14.1% in de-barked volume in year 4 was associated with cad-n1. Co-segregation analysis indicated that the cad locus itself might represent a gene that governs stem growth in pine. The significance of the mutation cad-n1 for tree growth and wood processing is discussed.

Key words Average effect CAD Lignin biosynthesis Loblolly pine Mutant RAPDs 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • R. L. Wu
    • 1
  • D. L. Remington
    • 2
  • J. J. MacKay
    • 3
  • S. E. McKeand
    • 4
  • D. M. O’Malley
    • 1
  1. 1.Forest Biotechnology Group and Department of Forestry, North Carolina State UniversityRaleighUS
  2. 2.Forest Biotechnology Group, Department of Forestry and Department of Genetics, North Carolina State UniversityRaleighUS
  3. 3.Institute of Paper Science and TechnologyAtlantaUS
  4. 4.Department of Forestry, North Carolina State UniversityRaleighUS

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