Advertisement

Theoretical and Applied Genetics

, Volume 99, Issue 3–4, pp 554–560 | Cite as

Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat

  • R. Seyfarth
  • C. Feuillet
  • G. Schachermayr
  • M. Winzeler
  • B. Keller
Article

Abstract 

The objective of this work was to develop a marker for the adult plant leaf rust resistance gene Lr35. The Lr35 gene was originally introgressed into chromosome 2B from Triticum speltoides, a diploid relative of wheat. A segregating population of 96 F 2 plants derived from a cross between the resistant line ThatcherLr35 and the susceptible variety Frisal was analysed. Out of 80 RFLP probes previously mapped on wheat chromosome 2B, 51 detected a polymorphism between the parents of the cross. Three of them were completely linked with the resistance gene Lr35. The co-segregating probe BCD260 was converted into a PCR-based sequence-tagged-site (STS) marker. A set of 48 different breeding lines derived from several European breeding programs was tested with the STS marker. None of these lines has a donor for Lr35 in its pedigree and all of them reacted negatively with the STS marker. As no leaf rust races virulent on Lr35 have been found in different areas of the world, the STS marker for the Lr35 resistance gene is of great value to support the introgression of this gene in combination with other leaf rust (Lr) genes into breeding material by marker-assisted selection.

Key words Leaf rust Adult plant resistance Sequence-tagged-site Triticum speltoides Wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • R. Seyfarth
    • 1
  • C. Feuillet
    • 1
  • G. Schachermayr
    • 2
  • M. Winzeler
    • 2
  • B. Keller
    • 1
  1. 1.Institute of Plant Biology, University of ZürichZürichCH
  2. 2.Department of Resistance and Quality Breeding, Swiss Federal Research Station for Agroecology and AgricultureZürichCH

Personalised recommendations