Theoretical and Applied Genetics

, Volume 99, Issue 1–2, pp 314–322 | Cite as

Strict paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in intraspecific crosses of kiwifruit

  • J. Chat
  • L. Chalak
  • R. J. Petit

Abstract

 Previous studies have established that chloroplasts are inherited paternally in Actinidia interspecific crosses. However, fertilisation problems in interspecific crosses may affect the transmission of organelles. Six female clones, i.e. ‘Abbott’, ‘Bruno’, ‘Greensill’, ‘Hayward’, ‘Jones’, ‘Monty’, and four male clones were used to identify cpDNA polymorphisms within the cultivated kiwifruit species A. deliciosa. The restriction patterns by HpaII of a chloroplast fragment amplified by PCR with a pair of universal primers revealed a polymorphism at the intraspecific level. The inheritance of cpDNA in 143 seedlings from three intraspecific crosses in kiwifruit (Actinidia deliciosa) was studied. All offspring displayed the restriction pattern of the paternal parent, indicating that maternal inheritance of cpDNA in kiwifruit is rare at best. Strict maternal inheritance of mtDNA was confirmed in the same crosses used to investigate cpDNA transmission. Studies of cytoplasmic inheritance in the Actinidia genus represent to date the best documented report of differential organelle inheritance of cpDNA and mtDNA in angiosperms.

Key words Actinidia deliciosa cpDNA mtDNA PCR Universal primers 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • J. Chat
    • 1
  • L. Chalak
    • 2
  • R. J. Petit
    • 3
  1. 1.Unité de Recherches sur les Espèces Fruitières et la Vigne, INRA, B.P. 81, F-33883 Villenave d’Ornon cedex, France Fax: 33-55-68 43 083 E-mail: chat@bordeaux.inra.frFR
  2. 2.S.R.A. San Giuliano, INRA-CIRAD 20230 San Nicolao, FranceFR
  3. 3.Laboratoire de Génétique et d’Amélioration des Arbres Forestiers, INRA, B.P. 45, F-33611 Gazinet Cedex, FranceFR

Personalised recommendations