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Theoretical and Applied Genetics

, Volume 99, Issue 1–2, pp 254–271 | Cite as

An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum×L. pennellii F2 populations

  • J. P. W. Haanstra
  • C. Wye
  • H. Verbakel
  • F. Meijer-Dekens
  • P. van den Berg
  • P. Odinot
  • A. W. van Heusden
  • S. Tanksley
  • P. Lindhout
  • J. Peleman

Abstract

 Two independent F2 populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for genetic and breeding purposes and map-based cloning.

Key words Molecular markers Integrated linkage map Tomato Lycopersicon species AFLP RFLP 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • J. P. W. Haanstra
    • 1
  • C. Wye
    • 2
  • H. Verbakel
    • 2
  • F. Meijer-Dekens
    • 1
  • P. van den Berg
    • 1
  • P. Odinot
    • 3
  • A. W. van Heusden
    • 3
  • S. Tanksley
    • 4
  • P. Lindhout
    • 1
  • J. Peleman
    • 2
  1. 1.Graduate School of Experimental Plant Sciences, Laboratory of Plant Breeding, Wageningen Agricultural University, Lawickse Allee 166, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31(0)317 483457 E-mail: jair.haanstra@users.pv.wau.nlNL
  2. 2.Keygene N.V., Agro Business Park 90, P.O. Box 216, 6700 AE Wageningen, The NetherlandsNL
  3. 3.Centre for Plant Breeding and Reproduction Research (CPRO-DLO) Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The NetherlandsNL
  4. 4.Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14853, USAUS

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