Advertisement

Theoretical and Applied Genetics

, Volume 99, Issue 1–2, pp 192–198 | Cite as

Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat

  • G. J. Bryan
  • P. Stephenson
  • A. Collins
  • J. Kirby
  • J. B. Smith
  • M. D. Gale

Abstract

 PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of results may be complicated by the simultaneous amplification of orthologous and paralogous sequences. These findings have significant implications for the use of STS markers in wheat and other polyploid species.

Key words PCR markers Sequence-tagged-site Wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • G. J. Bryan
    • 1
  • P. Stephenson
    • 1
  • A. Collins
    • 1
  • J. Kirby
    • 1
  • J. B. Smith
    • 1
  • M. D. Gale
    • 1
  1. 1.John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UJ, UK Fax: +44 1382 562426 E-mail: g.bryan@scri.sari.ac.ukGB

Personalised recommendations