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Theoretical and Applied Genetics

, Volume 98, Issue 2, pp 285–290 | Cite as

Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

  • A. Graner
  • S. Streng
  • A. Kellermann
  • A. Schiemann
  • E. Bauer
  • R. Waugh
  • B. Pellio
  • F. Ordon

Abstract

 The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.

Key words Disease resistance Hordeum vulgare Marker-assisted selection Molecular markers PCR 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • A. Graner
    • 1
  • S. Streng
    • 1
  • A. Kellermann
    • 2
  • A. Schiemann
    • 3
  • E. Bauer
    • 2
  • R. Waugh
    • 4
  • B. Pellio
    • 3
  • F. Ordon
    • 3
  1. 1.Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Gatersleben, Germany E-mail: graner@ipk-gatersleben.deDE
  2. 2.Institute for Resistance Genetics, Graf-Seinsheim-Straße 23, D-85461 Grünbach, GermanyDE
  3. 3.Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Ludwigstraße 23, D-35390 Giessen, GermanyDE
  4. 4.Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United KingdomGB

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