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Theoretical and Applied Genetics

, Volume 98, Issue 2, pp 179–185 | Cite as

Localization and activity of rRNA genes on fenugreek (Trigonella foenum-graecum L.) chromosomes by fluorescent in situ hybridization and silver staining

  • F. Ahmad
  • S. N. Acharya
  • Z. Mir
  • P. S. Mir

Abstract

 Fenugreek (Trigonella foenum-graecum L.), an annual legume crop grown mainly for seed at present, has the potential to be a high-quality forage crop in western Canada. A cytological survey of germplasm stored at the USDA germplasm center demonstrated a somatic karyotype with a chromosome number of 2n=16. Structural details of two pairs of chromosomes (numbers 1 and 2) showed secondary constrictions that were presumed to be the site of rRNA genes. Fluorescent in situ hybridization (FISH) with a heterologous rRNA gene probe from wheat was utilized to physically map their location for the first time on fenugreek chromosomes. Strong hybridization signals of apparently similar copy-number sequences were detected primarily near the centromere on the metacentric chromosome 1 and near the telomere on the acrocentric chromosome 2. Silver staining of the nucleolar organizing region provided evidence that both the sites were transcriptionally active and independently capable of forming a nucleolus. However, the site on chromosome number 2 was relatively more active than the one on chromosome number 1.

Key words Trigonella Fenugreek NORs Fluorescent in situ hybridization Genome mapping 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • F. Ahmad
    • 1
  • S. N. Acharya
    • 2
  • Z. Mir
    • 2
  • P. S. Mir
    • 2
  1. 1.Botany Department, Brandon University, Brandon, Manitoba R7A 6A9, Canada Fax: +1-204-728-7346 E-mail: ahmad@brandonu.caCA
  2. 2.Agriculture and Agri-Food Canada, Lethbridge, Alberta, CanadaCA

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