Theoretical and Applied Genetics

, Volume 97, Issue 8, pp 1235–1239

Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth)

  • V. Ivandic
  • U. Walther
  • A. Graner

DOI: 10.1007/s001220051015

Cite this article as:
Ivandic, V., Walther, U. & Graner, A. Theor Appl Genet (1998) 97: 1235. doi:10.1007/s001220051015

Abstract

 A dominant gene conferring resistance to all known races of Puccinia hordei Otth was identified in two accessions of Hordeum vulgare ssp. spontaneum. Using restriction fragment length polymorphism (RFLP) markers the gene was mapped on chromosome 2HS in doubled-haploid populations derived from crosses of both accessions to the susceptible cultivar L94. Until now, complete leaf rust resistance was not known to be conditioned by genetic factors on this barley chromosome. Therefore, the designation Rph16 is proposed for the gene described in this study. A series of sequence tagged site (STS) and cleaved amplified polymorphic sequence (CAPS) markers were generated by conversion of RFLP probes which originate from the chromosomal region carrying the resistance gene. Two PCR-based markers were shown to co-segregate with the Rph16 gene in both populations thus providing the basis for marker-assisted selection.

Key words CAPS Disease resistance Hordeum vulgare ssp. spontaneum RFLP-mapping STS 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • V. Ivandic
    • 1
  • U. Walther
    • 2
  • A. Graner
    • 1
  1. 1.Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, GermanyDE
  2. 2.Federal Centre for Breeding Research on Cultivated Plants, Institute for Epidemiology and Resistance, 06499 Aschersleben, GermanyDE

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