Theoretical and Applied Genetics

, Volume 97, Issue 8, pp 1204–1209

In situ hybridization identifies the diploid progenitor species of Coffea arabica (Rubiaceae)

  • S. N. Raina
  • Y. Mukai
  • M. Yamamoto

DOI: 10.1007/s001220051011

Cite this article as:
Raina, S., Mukai, Y. & Yamamoto, M. Theor Appl Genet (1998) 97: 1204. doi:10.1007/s001220051011

Abstract

 The most important commercial coffee species, Coffea arabica, which is cultivated in about 70% of the plantations world-wide, is the only tetraploid (2n=4x=44) species known in the genus. Genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) were used to study the genome organization and evolution of this species. Labelled total genomic DNA from diploid species (C. eugenioides, C. congensis, C. canephora, C. liberica) closely related to C. arabica was separately used as a probe in combination with or without blocking DNA to the chromosome spreads of C. arabica. GISH discriminated between chromosomes of C. arabica only in the presence of an excess of unlabelled block DNA from the species not used as a probe. Among the range of different species combinations used, DNA from C. eugenioides strongly and preferentially labelled 22 chromosomes of the tetraploid C. arabica, while the remaining 22 chromosomes were labelled with C. congensis DNA. The similarity of observations between C. arabica and the two diploid species using two ribosomal genes with FISH with respect to metaphase chromosomes provided additional support to the GISH results. These results confirm the allopolyploid nature of C. arabica and show that C. congensis and C. eugenioides are the diploid progenitors of C. arabica.

Key words Coffea arabica In situ hybridization Genomic probe Ribosomal DNA probes Allopolyploidy Wild progenitors 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • S. N. Raina
    • 1
  • Y. Mukai
    • 1
  • M. Yamamoto
    • 2
  1. 1.Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, 4-698-1, Asahigaoka, Kashiwara, Osaka 582, JapanJP
  2. 2.Department of Life Sciences, Kansai Women’s College, 3-11-1, Asahigaoka, Kashiwara, Osaka 582, JapanJP

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