Theoretical and Applied Genetics

, Volume 97, Issue 7, pp 1090–1103

A genetic linkage map of Quercus robur L. (pedunculate oak) based on RAPD, SCAR, microsatellite, minisatellite, isozyme and 5S rDNA markers

  • T. Barreneche
  • C. Bodenes
  • C. Lexer
  • J.-F. Trontin
  • S. Fluch
  • R. Streiff
  • C. Plomion
  • G. Roussel
  • H. Steinkellner
  • K. Burg
  • J.-M. Favre
  • J. Glössl
  • A. Kremer

DOI: 10.1007/s001220050996

Cite this article as:
Barreneche, T., Bodenes, C., Lexer, C. et al. Theor Appl Genet (1998) 97: 1090. doi:10.1007/s001220050996

Abstract

 A genetic map of Pedunculate oak (Quercus robur) was constructed based on one 5S rDNA, 271 RAPD, ten SCAR, 18 microsatellite, one minisatellite, and six isozyme markers. A total of 94 individuals from a full-sib family was genotyped. Two maps, including 307 markers, were constructed according to the “two-way pseudo-testcross” mapping strategy. Testcross markers segregating in the 1 : 1 ratio were first used to establish separate maternal (893.2 cM, 12 linkage groups) and paternal (921.7 cM, 12 linkage groups) maps. Both maps provided 85–90% genome coverage. Homologies between the male and female linkage groups were then identified based on 74 intercross markers segregating in the 3 : 1, 1 : 2 : 1 and 1 : 1 : 1 : 1 ratios (RAPDs, SCARs, SSRs, 5S rDNA and isozymes) in the hybrid progeny. In each map, approximately 18% of the studied markers showed segregation distortion. More than 60% of the skewed markers were due to an excess of heterozygote genotypes. This map will be used for: (1) studying the molecular organisation of genomic regions involved in inter- and intraspecific differentiation in oaks and (2) identification of QTLs for adaptive traits.

Key words Quercus robur L Linkage map RAPD SCAR Microsatellite Minisatellite 5S rDNA Isozymes 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Barreneche
    • 1
  • C. Bodenes
    • 1
  • C. Lexer
    • 2
  • J.-F. Trontin
    • 3
  • S. Fluch
    • 4
  • R. Streiff
    • 1
  • C. Plomion
    • 1
  • G. Roussel
    • 1
  • H. Steinkellner
    • 2
  • K. Burg
    • 4
  • J.-M. Favre
    • 3
  • J. Glössl
    • 2
  • A. Kremer
    • 1
  1. 1.Laboratoire de Génétique et d’Amélioration des Arbres Forestiers, INRA, B.P. 45, Pierroton, F-33610 Cestas Cedex, France Fax: (33) 5 57 97 90 88 E-mail: kremer@pierroton.inra.frFR
  2. 2.Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, AustriaAT
  3. 3.Laboratoire de Biologie Forestière, associé INRA, Université Henri Poincaré, Nancy, B.P. 239, 54506 Vandoeuvre-lès-Nancy, FranceFR
  4. 4.Forschungszentrum Seibersdorf, A-2444 Seibersdorf, AustriaAT

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