Theoretical and Applied Genetics

, Volume 97, Issue 4, pp 633–641

Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum)

  • W. Spielmeyer
  • A. G. Green
  • D. Bittisnich
  • N. Mendham
  • E. S. Lagudah

DOI: 10.1007/s001220050939

Cite this article as:
Spielmeyer, W., Green, A., Bittisnich, D. et al. Theor Appl Genet (1998) 97: 633. doi:10.1007/s001220050939

Abstract

 An AFLP genetic linkage map of flax (Linum usitatissimum) was used to identify two quantitative trait loci (QTLs) on independent linkage groups with a major effect on resistance to Fusarium wilt, a serious disease caused by the soil pathogen Fusarium oxysporum (lini). The linkage map was constructed using a mapping population from doubled-haploid (DH) lines. The DH lines were derived from the haploid component of F2 haploid-diploid twin seed originating from a cross between a polyembryonic, low-linolenic-acid genotype (CRZY8/RA91) and the Australian cultivar ‘Glenelg’. The AFLP technique was employed to generate 213 marker loci covering approximately 1400 cM of the flax genome (n=15) with an average spacing of 10 cM and comprising 18 linkage groups. Sixty AFLP markers (28%) deviated significantly (P<0.05) from the expected segregation ratio. The map incorporated RFLP markers tightly linked to flax rust (Melamspora lini) resistance genes and markers detected by disease resistance gene-like sequences. The study illustrates the potential of the AFLP technique as a robust and rapid method to generate moderately saturated linkage maps, thereby allowing the molecular analysis of traits, such as resistance to Fusarium wilt, that show oligogenic patterns of inheritance.

Key words Flax Linum usitatissimum AFLP Genetic mapping 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • W. Spielmeyer
    • 1
  • A. G. Green
    • 1
  • D. Bittisnich
    • 2
  • N. Mendham
    • 3
  • E. S. Lagudah
    • 1
  1. 1.CSIRO Plant Industry, GPO 1600 Canberra ACT 2601, Australia Fax: +61 2 6246 5000 E-mail: wolfgang.spielmeyer@pi.csiro.auAU
  2. 2.Cooperative Research Centre for Plant Science, GPO Box 475, Canberra ACT 2601, AustraliaAU
  3. 3.Department of Agricultural Science, University of Tasmania, GPO Box, Hobart 7000, AustraliaAU

Personalised recommendations