Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Inheritance of somatic embryogenesis in red clover (Trifolium pratense L.)


 Red clover genotypes capable of regenerating plantlets in vitro from non-meristematic tissue-derived callus are rare. Selection for genotypes capable of somatic embryogenesis identified a clone comprised of a group of plantlets regenerated from a hypocotyl-derived callus culture on L2-based media and another group of plantlets originating from crown divisions of the epicotyl-derived plant. The callus-derived plants of this clone were highly regenerative when reintroduced to callus culture, but the epicotyl-derived plants produced nonregenerative callus cultures. F1, F2 and BC1 populations were evaluated to determine the mode of inheritance of the regeneration trait. Reciprocal crosses did not differ, indicating a lack of maternal effects. Results were compatible with genetic control of regeneration by two complementary genes. We propose the genotype Rn1-Rn2- for regenerative plants. Three petiole segment explants were sufficient to evaluate regenerative ability in seedlings. Regenerative ability was often associated with abnormal leaf morphology in a few to several leaves.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 17 February 1998 / Accepted: 7 April 1998

Rights and permissions

Reprints and Permissions

About this article

Cite this article

McLean, N., Nowak, J. Inheritance of somatic embryogenesis in red clover (Trifolium pratense L.). Theor Appl Genet 97, 557–562 (1998).

Download citation

  • Key words Red clover
  • Trifolium pratense
  • Somatic embryogenesis
  • Inheritance