Theoretical and Applied Genetics

, Volume 97, Issue 1–2, pp 60–73 | Cite as

Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers

  • C. Maliepaard
  • F. H. Alston
  • G. van Arkel
  • L. M. Brown
  • E. Chevreau
  • F. Dunemann
  • K. M. Evans
  • S. Gardiner
  • P. Guilford
  • A. W. van Heusden
  • J. Janse
  • F. Laurens
  • J. R. Lynn
  • A. G. Manganaris
  • A. P. M. den Nijs
  • N. Periam
  • E. Rikkerink
  • P. Roche
  • C. Ryder
  • S. Sansavini
  • H. Schmidt
  • S. Tartarini
  • J. J. Verhaegh
  • M. Vrielink-van Ginkel
  • G. J. King

Abstract

 Linkage maps for the apple cultivars ‘Prima’ and ‘Fiesta’ were constructed using RFLP, RAPD, isozyme, AFLP, SCAR and microsatellite markers in a ‘Prima’בFiesta’ progeny of 152 individuals. Seventeen linkage groups, putatively corresponding to the seventeen haploid apple chromosomes, were obtained for each parent. These maps were aligned using 67 multi-allelic markers that were heterozygous in both parents. A large number of duplicate RFLP loci was observed and, in several instances, linked RFLP markers in one linkage group showed corresponding linkage in another linkage group. Distorted segregation was observed mainly in two regions of the genome, especially in the male parent alleles. Map positions were provided for resistance genes to scab and rosy leaf curling aphid (Vf and Sd1, respectively) for the fruit acidity gene Ma and for the self-incompatibility locus S. The high marker density and large number of mapped codominant RFLPs and some microsatellite markers make this map an ideal reference map for use in other progenies also and a valuable tool for the mapping of quantitative trait loci.

Key wordsMalus pumila Mill Molecular linkagemap Marker-assisted selection Fruit tree breeding Outbred progeny 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • C. Maliepaard
    • 1
  • F. H. Alston
    • 3
  • G. van Arkel
    • 1
  • L. M. Brown
    • 2
  • E. Chevreau
    • 6
  • F. Dunemann
    • 5
  • K. M. Evans
    • 3
  • S. Gardiner
    • 8
  • P. Guilford
    • 9
  • A. W. van Heusden
    • 1
  • J. Janse
    • 1
  • F. Laurens
    • 6
  • J. R. Lynn
    • 2
  • A. G. Manganaris
    • 7
  • A. P. M. den Nijs
    • 1
  • N. Periam
    • 2
  • E. Rikkerink
    • 9
  • P. Roche
    • 3
  • C. Ryder
    • 2
  • S. Sansavini
    • 4
  • H. Schmidt
    • 5
  • S. Tartarini
    • 4
  • J. J. Verhaegh
    • 1
  • M. Vrielink-van Ginkel
    • 1
  • G. J. King
    • 2
  1. 1.DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, The Netherlands Fax: 31 317 41 80 94 E-mail: c.a.maliepaard@cpro.dlo.nlNL
  2. 2.Horticulture Research International, Wellesbourne (HRI-W), Warwick CV35 9EF, UKGB
  3. 3.Horticulture Research International, East-Malling (HRI-EM), West Malling, Kent ME19 6BJ, UKGB
  4. 4.Dipartimento di Colture Arboree (DCA), University of Bologna, Via Filippo Re 6, 40126, Bologna, ItalyIT
  5. 5.Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding (IZZ), Bornkampsweg 31, D-22926, Ahrensburg, GermanyDE
  6. 6.INRA, Station d’Amélioration des espèces fruitières et ornementales, Centre de Recherches d’Angers, 49070 Beaucouzé, FranceFR
  7. 7.NAGREF, Pomology Institute, PO Box 122, 592 00, Naoussa, Makedonia, GreeceGR
  8. 8.Horticulture and Food Research Institute of New Zealand, Palmerston North Research Centre, Private Bag 11 030, Palmerston North, NZNZ
  9. 9.Horticulture and Food Research Institute of New Zealand, Mt. Albert Research Centre, Private Bag 92 169, Auckland, NZNZ

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