Theoretical and Applied Genetics

, Volume 96, Issue 8, pp 1170–1177 | Cite as

Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease

  • N. G. Paltridge
  • N. C. Collins
  • A. Bendahmane
  • R. H. Symons

Abstract

 The Yd2 gene in barley provides protection against barley yellow dwarf luteovirus (BYDV), the most economically devastating virus of cereals worldwide. Because resistance assays to identify Yd2-containing individuals from breeding populations are often difficult, we have developed a closely linked, codominant PCR-based marker for Yd2 using AFLP marker technology. The marker, designated YLM, can be amplified from barley genomic DNA prepared using a rapid and simple extraction procedure and, in a survey of more than 100 barley genotypes, was found to be polymorphic between most Yd2 and non-Yd2 lines. The YLM therefore shows excellent potential as a tool for selecting Yd2-carrying segregants in barley breeding programmes.

Key words AFLP Barley yellow dwarf virus Hordeum vulgare PCR marker Yd2 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • N. G. Paltridge
    • 1
  • N. C. Collins
    • 1
  • A. Bendahmane
    • 2
  • R. H. Symons
    • 1
  1. 1.Department of Plant Science, Waite Institute, The University of Adelaide, Glen Osmond, SA 5064, AustraliaAU
  2. 2.The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UKGB

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