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Theoretical and Applied Genetics

, Volume 96, Issue 6–7, pp 950–956 | Cite as

Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides)

  • S. Dayanandan
  • O. P. Rajora
  • K. S. Bawa

Abstract

 We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Populus species. Fourteen microsatellites were identified from 1600 clones screened. The TC/AG microsatellites were the most abundant. A total of 29 alleles were detected in 36 P. tremuloides individuals at the four SSR loci (two each of di- and tri-nucleotide repeats) characterized. The number of alleles at the SSR loci ranged from 5 to 11, with an average of 7.25 alleles per locus, and the observed heterozygosity ranged from 0.19 to 0.82, with a mean of 0.46 per locus. Although the highest polymorphism was observed for a dinucleotide SSR locus, the trinucleotide SSR loci showed substantial polymorphism. There were 34 unique multilocus genotypes among the 36 P. tremuloides individuals examined, and 89% of the individuals had unique multilocus genotypes. Two pairs of SSR primers were successful in PCR, amplifying genomic DNA and resolving microsatellites of comparable size from Populus deltoides, P. nigra, Pcanadensis, and P. maximowiczii. The microsatellite DNA markers developed could be used for clonal fingerprinting, certification of controlled crosses, genome mapping, marker-assisted early selection, genetic diversity assessments, and conservation and sustainable management of poplar genetic resources.

Key words Poplars (Populus) Simple sequencerepeats Microsatellite loci Polymorphism Clone identification 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • S. Dayanandan
    • 1
  • O. P. Rajora
    • 2
  • K. S. Bawa
    • 1
  1. 1.Biology Department, University of Massachusetts, 100 Morrisey Blvd, Boston, MA 02125, USAUS
  2. 2.Department of Renewable Resources, 751 General Services Building, University of Alberta, Edmonton, Alberta, T6G 2H1 Canada Fax: +1-403 492-4323 E-mail: om.rajora@ualberta.caCA

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