Theoretical and Applied Genetics

, Volume 96, Issue 3–4, pp 435–446

Genetic diversity in Australian wheat varieties and breeding material based on RFLP data

  • J. G. Paull
  • K. J. Chalmers
  • A. Karakousis
  • J. M. Kretschmer
  • S. Manning
  • P. Langridge
Article

DOI: 10.1007/s001220050760

Cite this article as:
Paull, J., Chalmers, K., Karakousis, A. et al. Theor Appl Genet (1998) 96: 435. doi:10.1007/s001220050760

Abstract

Restriction fragment length polymorphisms (RFLPs) have been used to characterise the genetic diversity of wheat (Triticum aestivum) germplasm. One hundred and twenty-four accessions comprising all major Australian wheat varieties and lines important for breeding purposes were assayed for RFLPs with clones of known genetic location and selected to give uniform genome coverage. The objectives of this study were to determine RFLP-based genetic similarity between accessions and to derive associations between agronomically significant traits and RFLP phenotypes. Ninety-eight probes screened against genomic DNA digested with five restriction endonucleases detected a total of 1968 polymorphic fragments. Genetic similarity (GS) calculated from the RFLP data ranged from 0.004 to 0.409 between accessions, with a mean of 0.18. Cluster analysis based on GS estimates produced four groupings that were generally consistent with available pedigree information. Comparisons of the RFLP phenotypes of accessions containing disease resistance genes present on introgressed alien segments enabled the identification of specific alleles characteristic of these regions. Associations were derived for a range of stem-rust, leaf-rust and yellow-rust resistance genes. These results suggest that RFLP analysis can be used for the characterisation and grouping of elite breeding material of wheat and RFLP profiling can identify chromosome segments associated with agronomic traits.

Key words RFLP analysis Triticum aestivum Genetic diversity Genetic similarity estimates Cluster analysis 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. G. Paull
    • 1
  • K. J. Chalmers
    • 2
  • A. Karakousis
    • 2
  • J. M. Kretschmer
    • 2
  • S. Manning
    • 2
  • P. Langridge
    • 2
  1. 1.Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064, AustraliaAU
  2. 2.ARC Special Research Centre for Basic and Applied Plant Molecular Biology, Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064 Fax: 618 8303 7123 E-mail: Rchalmers@waite.adelaide.edu.auAU

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