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Theoretical and Applied Genetics

, Volume 96, Issue 3–4, pp 325–330 | Cite as

Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes

  • K. Fukui
  • S. Nakayama
  • N. Ohmido
  • H. Yoshiaki
  • M. Yamabe
Article

Abstract

Chromosomes of the three diploid Brassica species, B. rapa (AA), B. nigra (BB) and B. oleracea (CC), were identified based on their morphological characteristics, especially on the condensation pattern appearing at the somatic pro-metaphase stage. The morphological features of the pro-metaphase chromosomes of the three Brassica spp. were quantified by imaging methods using chromosome image analyzing system II (CHIAS 2). As a result, quantitative chromosome maps or idiograms of the three diploid Brassica spp. were developed. The fluorescence in situ hybridization (FISH) method revealed the location of 45s rDNA (the 26s-5.8s-18s ribosomal RNA gene cluster) on the chromosomes involved. The number of 45s rDNA loci in the B. rapa, B. nigra and B. oleracea are five, three and two, respectively. The loci detected were systematically mapped on the idiograms of the three Brassica spp.

Key words Brassica rapa Brassica nigra Brassica oleracea Quantitative chromosome map Idiogram FISH 45s rDNA Systematic mapping 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • K. Fukui
    • 1
  • S. Nakayama
    • 2
  • N. Ohmido
    • 1
  • H. Yoshiaki
    • 3
  • M. Yamabe
    • 3
  1. 1.Hokuriku National Agricultural Experiment Station, Joetsu 943-01, Japan Fax: 81-255-26-3238 E-mail: kfukui@inada.affrc.go.jpJP
  2. 2.National Institute of Agrobiological Resources, Tsukuba 305, JapanJP
  3. 3.Ishikawa Prefectural Agricultural Center, Kanazawa 920-01, JapanJP

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