Theoretical and Applied Genetics

, Volume 95, Issue 8, pp 1239–1245 | Cite as

Identification of the rice D-genome chromosomes by genomic in situ hybridisation

  • K. Fukui
  • R. Shishido
  • T. Kinoshita

Abstract

 The 24 rice D-genome chromosomes were identified among the 48 chromosomes of O. latifolia, which comprise the C- and D-genomes, using genomic in situ hybridisation (GISH). The B-genome chromosomes were also discriminated from the C-genome chromosomes in O. minuta (BBCC) by GISH. A comparison of the differences in the fluorescence intensity between the C and D genomes within O. latifolia (CCDD), and between the B and C genomes within O. minuta, indicated that the overall nucleotide-sequence homology between the B and C genomes is less than that between the C and D genomes. The origin of the D genome and the phylogenetic relationship of the D genome among the rice genomes are discussed, based on the results obtained.

Key words Genomic in situ hybridisation (GISH) Genus Oryza O. latifolia (CCDD) O. minuta (BBCC) Rice D genome 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • K. Fukui
    • 1
  • R. Shishido
    • 2
  • T. Kinoshita
    • 3
  1. 1.Hokuriku National Agricultural Experiment Station, Joetsu 943-01, Japan Fax: +81-255-24-8578 Email: kfukui@inada.affrc.go.jpJP
  2. 2.Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, Sapporo 060, JapanJP
  3. 3.Hokkaido University, Sapporo 060, JapanJP

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