Theoretical and Applied Genetics

, Volume 95, Issue 8, pp 1210–1217

A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines

  • C. M. Menéndez
  • A. E. Hall
  • P. Gepts

DOI: 10.1007/s001220050683

Cite this article as:
Menéndez, C., Hall, A. & Gepts, P. Theor Appl Genet (1997) 95: 1210. doi:10.1007/s001220050683

Abstract

 We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively. A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other traits in the cultivated gene pool of cowpea.

Key words Vigna unguiculata RFLP RAPD AFLP Linkage map 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • C. M. Menéndez
    • 1
  • A. E. Hall
    • 2
  • P. Gepts
    • 1
  1. 1.Department of Agronomy and Range Science, University of California, Davis, CA 95616-8515, USA E-mail: plgepts@ucdavis.eduUS
  2. 2.Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92521, USAUS

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