Linkages between high- and low-molecular-weight (Mr) glutenin, gliadin and triticin loci in diploid, tetraploid and hexaploid wheats were studied by hybridization of restriction fragments with DNA clones and by SDS-PAGE. In tetraploid and hexaploid wheat, DNA fragments hybridizing with a low-Mr glutenin clone were mapped at the XGlu-3 locus in the distal region of the maps of chromosome arms 1AS, 1BS, and 1DS. A second locus, designated XGlu-B2, was detected in the middle of the map of chromosome arm 1BS completely linked to the XGli-B3 gliadin locus. The restriction fragments mapped at this locus were shown to co-segregate with B subunits of low-Mr glutenins in SDS-PAGE in tetraploid wheat, indicating that XGlu-B2 is an active low-Mr glutenin locus. A new locus hybridizing with the low-Mr clone was mapped on the long arm of chromosome 7Am in diploid wheat. No glutenin protein was found to co-segregate with this new locus. Triticin loci were mapped on chromosome arms 1AS, 1BS, and 1DS. A failure to detect triticin proteins co-segregating with DNA fragments mapped at XTri-B1 locus suggests that this locus is not active. No evidence was found for the existence of Gli-A4, and it is concluded that this locus is probably synonymous with Gli-A3. Recombination was observed within the multigene gliadin family mapped at XGli-A11 (1.2 cM).1 Although these closely linked loci may correspond to the previously named Gli-A1 and Gli-A5 loci, they were temporarily designated XGli-A1.1 and XGli-A1.2 until orthology with Gli-A1 and Gli-A5 is established.
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Received: 25 March 1997 / Accepted: 23 June 1997
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Dubcovsky, J., Echaide, M., Giancola, S. et al. Seed-storage-protein loci in RFLP maps of diploid, tetraploid, and hexaploid wheat. Theor Appl Genet 95, 1169–1180 (1997). https://doi.org/10.1007/s001220050678
- Key words Triticum
- Storage-protein loci